白木香转录因子AsMYB1和AsMYB2克隆及表达分析(1)
[摘要] MYB类转录因子是数量最多、功能最多样化的转录因子家族之一。该研究根据白木香转录组高通量测序结果设计引物扩增白木香中MYB基因，利用RTPCR技术从白木香中首次克隆得到2个MYB基因cDNA 全长，分别命名为AsMYB1，AsMYB2，并对MYB蛋白质进行跨膜结构分析和聚类分析，预测其生物学信息；利用实时荧光定量PCR技术检测 AsMYB基因在不同组织， 及在NaCl、低温(4 ℃)、重金属(CdCl2)、干旱(甘露醇)4种非生物胁迫和脱落酸、水杨酸、赤霉素、茉莉酸甲酯4种外源激素处理下的表达差异。该研究中克隆获得的AsMYB1基因全长1 063 bp，编码353个氨基酸；AsMYB2基因全长1 081 bp，编码359个氨基酸。2个基因具有组织表达特异性；AsMYB1，AsMYB2 基因的转录水平受到不同生物胁迫和外源激素的影响，说明MYB基因对白木香防御反应和激素信号传导具有重要的作用。
[关键词] 白木香； MYB基因； 表达分析； 非生物胁迫； 激素处理
, 百拇医药
Cloning and expression analysis of transcription factor AsMYB1 and
AsMYB2 from Aquilaria sinensis
FENG Yingying， DONG Xianjuan， LIU Xiao， YAN Yaru， WANG Jinling， SHI Shepo， WANG Xiaohui*， TU Pengfei*
(Modern Research Center for Traditional Chinese Medicine， School of Chinese Materia Medica，Beijing University of Chinese Medicine， Beijing 100029， China)
[Abstract] The MYB gene family comprises one of the richest groups of transcription factors in plants. The full length of two MYB genes were isolated through heterologous screening of Aquilaria sinensis calli transcriptome data， and the reverse transcription PCR was performed to obstain the corrected MYB clones， named AsMYB1， AsMYB2. The MYB transmembrane domain and phylogenetic analysis were predicted by different software to analyze the bioinformatics of MYB proteins. The transcript level of AsMYB1， AsMYB2 was performed by realtime quantitative RTPCR in different tissues and in responds to abiotic stresses including salt， cold， metal and drought stress， and hormone treatments including abscisic acid (ABA)， salicylic acid (SA)， gibberellins (GA3) and methyl jasmonate (MeJA) treatment. The AsMYB1 cDNA sequence had an ORF of 1 063 nucleotides， encoding a protein of 353 amino acids. The largest AsMYB2 ORF was 1 081 nucleotides， and its predicted translation products consisted of 359 amino acids. Two MYB genes had a tissuesspecific pattern in A. sinensis. Moreover， the expression level of AsMYB1 and AsMYB2 was regulated by different abiotic stresses and hormone treatments， suggesting the transcription factors AsMYB1 and AsMYB2 play an important role in plant defense and hormone signal transduction in A. sinensis.
, 百拇医药
[Key words] Aquilaria sinensis； MYB transcription factor； expression analysis； abiotic stress； hormone treatment
轉录因子(transcription factor， TF)也称反式作用因子，是一类能够与基因启动子区域中顺式作用元件、沉默子或增强子发生特异性结合并相互作用，从而激活或者抑制目的基因转录和翻译的DNA结合蛋白。DNA 结合区域的特点可将转录因子分为若干个家族，其中，MYB(myeloblastosis)转录因子蛋白是包含 MYB 结构域且数量众多、功能多样的一类蛋白家族成员[1]，它们在植物的生长发育、次生代谢、激素信号转导和植物的非生物胁迫反应中起到重要的作用。对MYB基因克隆和功能进行研究，有利于丰富植物的防御反应机制和信号转导机制的研究。, http://www.100md.com(冯莹莹 董先娟 刘晓 闫雅如 王金铃 史社坡 王晓晖 屠鹏飞)
[关键词] 白木香； MYB基因； 表达分析； 非生物胁迫； 激素处理
, 百拇医药
Cloning and expression analysis of transcription factor AsMYB1 and
AsMYB2 from Aquilaria sinensis
FENG Yingying， DONG Xianjuan， LIU Xiao， YAN Yaru， WANG Jinling， SHI Shepo， WANG Xiaohui*， TU Pengfei*
(Modern Research Center for Traditional Chinese Medicine， School of Chinese Materia Medica，Beijing University of Chinese Medicine， Beijing 100029， China)
[Abstract] The MYB gene family comprises one of the richest groups of transcription factors in plants. The full length of two MYB genes were isolated through heterologous screening of Aquilaria sinensis calli transcriptome data， and the reverse transcription PCR was performed to obstain the corrected MYB clones， named AsMYB1， AsMYB2. The MYB transmembrane domain and phylogenetic analysis were predicted by different software to analyze the bioinformatics of MYB proteins. The transcript level of AsMYB1， AsMYB2 was performed by realtime quantitative RTPCR in different tissues and in responds to abiotic stresses including salt， cold， metal and drought stress， and hormone treatments including abscisic acid (ABA)， salicylic acid (SA)， gibberellins (GA3) and methyl jasmonate (MeJA) treatment. The AsMYB1 cDNA sequence had an ORF of 1 063 nucleotides， encoding a protein of 353 amino acids. The largest AsMYB2 ORF was 1 081 nucleotides， and its predicted translation products consisted of 359 amino acids. Two MYB genes had a tissuesspecific pattern in A. sinensis. Moreover， the expression level of AsMYB1 and AsMYB2 was regulated by different abiotic stresses and hormone treatments， suggesting the transcription factors AsMYB1 and AsMYB2 play an important role in plant defense and hormone signal transduction in A. sinensis.
, 百拇医药
[Key words] Aquilaria sinensis； MYB transcription factor； expression analysis； abiotic stress； hormone treatment
轉录因子(transcription factor， TF)也称反式作用因子，是一类能够与基因启动子区域中顺式作用元件、沉默子或增强子发生特异性结合并相互作用，从而激活或者抑制目的基因转录和翻译的DNA结合蛋白。DNA 结合区域的特点可将转录因子分为若干个家族，其中，MYB(myeloblastosis)转录因子蛋白是包含 MYB 结构域且数量众多、功能多样的一类蛋白家族成员[1]，它们在植物的生长发育、次生代谢、激素信号转导和植物的非生物胁迫反应中起到重要的作用。对MYB基因克隆和功能进行研究，有利于丰富植物的防御反应机制和信号转导机制的研究。, http://www.100md.com(冯莹莹 董先娟 刘晓 闫雅如 王金铃 史社坡 王晓晖 屠鹏飞)