15种重楼属植物中8种甾体皂苷的含量测定(1)
[摘要]建立了同時测定重楼中 8种重楼皂苷(重楼皂苷Ⅶ、H、Ⅵ、Ⅱ、Ⅰ、Ⅴ， 薯蓣皂苷和纤细薯蓣皂苷)的分析方法，并对15种重楼属植物根茎中重楼皂苷的含量进行了评价。本研究采用Waters Acquity HClass TM液相色谱系统，PDA检测器，色谱柱为资生堂CAPCELL PAK ADME 色谱柱(46 mm×250 mm， 5 μm)，乙腈水为流动相(08 mL·min-1)梯度洗脱，柱温21 ℃；检测波长为203 nm。可知①8种重楼皂苷得到良好分离，稳定性和重复性好，且线性良好(R2>0999 9)，线性范围较宽(0041 70～3812 00 μg)，平均回收率在9591%～1038%。②不同种重楼中重楼皂苷的含量差异显著，毛重楼、狭叶重楼和金线重楼中含量较低或基本不含重楼皂苷，不适合作为重楼药用；不同产地的七叶一枝花含量差异显著；滇重楼、长柱重楼、大理重楼和五指莲中重楼皂苷含量较高，最高达50%，长柱重楼、大理重楼和五指莲可作为潜在的重楼药用资源。
[关键词]重楼属； 甾体皂苷； 含量测定； 高效液相色谱法
, 百拇医药
Determination of eight steroidal saponins in 15 kinds of genus Paris
HUANG Yuanyuan1，2， LIU Dahui3， PENG Huasheng1， HAO Qingxiu2， CHEN Meilan2，ZHANG Azhong4， CHEN Min2， KANG Liping2*， HUANG Luqi2*
(1School of Pharmacy， Anhui University of Chinese Medicine， Hefei 230012， China；
2State Key Laboratory Breeding Base of Daodi Herbs， National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China；
, 百拇医药
3College of Pharmacy， Hubei University of Chinese Medicine， Wuhan 430065， China；
4Lanping County in Ecological Industry Co， Ltd， Nujiang 673100， China)
[Abstract]The study was established an HPLC method for simultaneous determination of 8 steroidal saponins (polyphyllins Ⅶ， H， Ⅵ， Ⅱ， Ⅰ， and Ⅴ， dioscin， and gracillin) in Paridis Rhizoma， and made an evaluation by determining steroidal saponins in 15 kinds of genus Paris The analysis was performed on a Waters Acquity HClassTM UPLC ultrafine liquid chromatography system coupled with a PDA detector The chromatographic separation was achieved through a CAPCELL PAK ADME (46 mm× 250 mm， 5 μm) column and the optimal mobile phase consisted of acetonitrile and water The column was maintained at 21 ℃， and the flow rate was 08 mL·min-1 The UV detection wavelength was 203 nm The results showed that ① the detected components can be well separated and all with good correlation coefficients The standard calibration curves were linearly good (R2>0999 9) The linearity was obtained over 0041 703812 00 μg The average recoveries ranged from 9591% to 1038% ② there are significant differences in the content of steroidal saponins from different species The steroidal saponins are low content or almost none in P. mairei， P. polyphylla var stenophylla， and P. delavayi have low content or almost did not contain， so these species are not suitable for medicinal use The contents of steroidal saponins in P. polyphylla var chinensis are varied from different places There were high content of steroidal saponins in P. polyphylla var yunnanensis， P. forrestii， P. daliensis， and P. axialis， even up to 50%， which indicated that they had the potential pharmic value of development, http://www.100md.com(黄圆圆刘大会彭华胜郝庆秀陈美兰张啊中陈敏康利平黄璐琦)
[关键词]重楼属； 甾体皂苷； 含量测定； 高效液相色谱法
, 百拇医药
Determination of eight steroidal saponins in 15 kinds of genus Paris
HUANG Yuanyuan1，2， LIU Dahui3， PENG Huasheng1， HAO Qingxiu2， CHEN Meilan2，ZHANG Azhong4， CHEN Min2， KANG Liping2*， HUANG Luqi2*
(1School of Pharmacy， Anhui University of Chinese Medicine， Hefei 230012， China；
2State Key Laboratory Breeding Base of Daodi Herbs， National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China；
, 百拇医药
3College of Pharmacy， Hubei University of Chinese Medicine， Wuhan 430065， China；
4Lanping County in Ecological Industry Co， Ltd， Nujiang 673100， China)
[Abstract]The study was established an HPLC method for simultaneous determination of 8 steroidal saponins (polyphyllins Ⅶ， H， Ⅵ， Ⅱ， Ⅰ， and Ⅴ， dioscin， and gracillin) in Paridis Rhizoma， and made an evaluation by determining steroidal saponins in 15 kinds of genus Paris The analysis was performed on a Waters Acquity HClassTM UPLC ultrafine liquid chromatography system coupled with a PDA detector The chromatographic separation was achieved through a CAPCELL PAK ADME (46 mm× 250 mm， 5 μm) column and the optimal mobile phase consisted of acetonitrile and water The column was maintained at 21 ℃， and the flow rate was 08 mL·min-1 The UV detection wavelength was 203 nm The results showed that ① the detected components can be well separated and all with good correlation coefficients The standard calibration curves were linearly good (R2>0999 9) The linearity was obtained over 0041 703812 00 μg The average recoveries ranged from 9591% to 1038% ② there are significant differences in the content of steroidal saponins from different species The steroidal saponins are low content or almost none in P. mairei， P. polyphylla var stenophylla， and P. delavayi have low content or almost did not contain， so these species are not suitable for medicinal use The contents of steroidal saponins in P. polyphylla var chinensis are varied from different places There were high content of steroidal saponins in P. polyphylla var yunnanensis， P. forrestii， P. daliensis， and P. axialis， even up to 50%， which indicated that they had the potential pharmic value of development, http://www.100md.com(黄圆圆刘大会彭华胜郝庆秀陈美兰张啊中陈敏康利平黄璐琦)