甘草色泽与有效成分含量的相关性研究(1)
[摘要] 为探讨甘草色泽与其质量评价的相关性，该研究以精密色差仪和视觉分析仪测定甘草根皮和断面颜色，以HPLC测定甘草药材中6种黄酮类和2种皂苷类成分的含量，采用偏最小二乘回归法对两类数据进行相关分析。结果发现，野生和栽培甘草根皮颜色不存在显著差异，断面颜色存在显著或极显著差异(P<0.05，P<0.01)；野生甘草的甘草苷、异甘草苷、异甘草素含量与甘草酸铵、甘草次酸含量显著或极显著高于栽培甘草；相关性结果表明，甘草根皮和断面的颜色均与有效成分含量具有显著或极显著相关性。该研究为传统以甘草色泽评价其质量提供了科学依据，为完善中药材质量评价体系提供新的参考。
[关键词] 甘草； 色泽； 含量测定； 相关性分析
[Abstract] To explore the correlation between color of Glycyrrhiza uralensis and its quality evaluation，the colors of root bark and transverse section were determined by Precision Color Reader and Visual Analyzer，and the contents of six flavonoids and two saponins in G.uralensis were determined by high performance liquid chromatography(HPLC).The partial least squares regression(PLSR)method was employed to correlate the colors with component contents in G.uralensis. The results showed that there were no significant differences in the colors of root bark but significant or very significant differences(P<0.05，P<0.01)in the colors of transverse section between the wild and cultivated G. uralensis. Compared with those in the cultivated G. uralensis， the contents of liquiritin， isoliquiritin isoliquiritigenin and the contents of ammonium glycyrrhizinate， glycyrrhetinic acid were obviously significant or remarkably significant in the wild G. uralensis.The correlation results showed that there was a significant or very significant correlation between the colors and the effective component contents. This study provides a scientific basis to evaluate the quality of G.uralensis by color and a new reference for the traditional evaluation methods for Chinese drugs.
, 百拇医药
[Key words] Glycyrrhiza uralensis； color； determination； correlation analysis
甘草為豆科植物甘草Glycyrrhiza uralensis Fisch.、胀果甘草G. inflate Bat.或光果甘草G. glabra L.的干燥根和根茎。主产于新疆、内蒙古、甘肃、宁夏等省区，是临床常用的大宗药材。由于掠夺性采挖，甘草野生资源遭到了严重的破坏，栽培甘草目前已经成为甘草药材商品的主要来源[1]。在中药材出口流通领域普遍认为栽培甘草质量不及野生甘草[2]。实际工作中，甘草质量常采用外观评价，以外皮细紧、色红棕、质坚实、体重、断面黄白、粉性足、味甜者为佳[3]。其中，根皮和断面颜色是品质评价最直观的指标。然而，色泽的经验鉴别依赖于人的主观感受，缺乏客观量化指标，传承和应用均存在困难。本文以主产地的野生和栽培乌拉尔甘草为研究对象，采用精密色差仪和视觉分析仪对甘草根皮和断面颜色进行客观的量化分析，采用HPLC对甘草中8种有效成分进行定量分析，将两部分数据采用偏最小二乘回归法进行相关分析，并对相关结果进行比较与验证，探究色泽与有效成分含量的相关性，阐释传统色泽鉴别作为甘草质量评价标准的科学内涵。
1 材料
HP-C220型精密色差仪[汉谱(上海)光电科技有限公司]；VA400型IRIS视觉分析仪[ 阿默思(上海)仪器贸易有限公司]；Agilent 1100型高效液相色谱仪(四元梯度泵、自动进样器、柱温箱和二极管阵列检测器，Agilent科技有限公司)；EYELAN-1100旋转蒸发仪(上海爱朗仪器有限公司)；HHS电热恒温水浴锅(上海博讯实业有限公司医疗设备厂出品)；VO 400真空干燥箱(德国memmert)；DV215CD型电子分析天平(OHAUS CORPORATION，USA)；KQ-500E型超声波清洗器(昆山市超声仪器有限公司)；电加热套(上海一科仪器有限公司)。, 百拇医药(马婷婷 龚慕辛 王智民 贺蕊 吴莎 张村 李静 李朝霞 陆允 许永崧)
[关键词] 甘草； 色泽； 含量测定； 相关性分析
[Abstract] To explore the correlation between color of Glycyrrhiza uralensis and its quality evaluation，the colors of root bark and transverse section were determined by Precision Color Reader and Visual Analyzer，and the contents of six flavonoids and two saponins in G.uralensis were determined by high performance liquid chromatography(HPLC).The partial least squares regression(PLSR)method was employed to correlate the colors with component contents in G.uralensis. The results showed that there were no significant differences in the colors of root bark but significant or very significant differences(P<0.05，P<0.01)in the colors of transverse section between the wild and cultivated G. uralensis. Compared with those in the cultivated G. uralensis， the contents of liquiritin， isoliquiritin isoliquiritigenin and the contents of ammonium glycyrrhizinate， glycyrrhetinic acid were obviously significant or remarkably significant in the wild G. uralensis.The correlation results showed that there was a significant or very significant correlation between the colors and the effective component contents. This study provides a scientific basis to evaluate the quality of G.uralensis by color and a new reference for the traditional evaluation methods for Chinese drugs.
, 百拇医药
[Key words] Glycyrrhiza uralensis； color； determination； correlation analysis
甘草為豆科植物甘草Glycyrrhiza uralensis Fisch.、胀果甘草G. inflate Bat.或光果甘草G. glabra L.的干燥根和根茎。主产于新疆、内蒙古、甘肃、宁夏等省区，是临床常用的大宗药材。由于掠夺性采挖，甘草野生资源遭到了严重的破坏，栽培甘草目前已经成为甘草药材商品的主要来源[1]。在中药材出口流通领域普遍认为栽培甘草质量不及野生甘草[2]。实际工作中，甘草质量常采用外观评价，以外皮细紧、色红棕、质坚实、体重、断面黄白、粉性足、味甜者为佳[3]。其中，根皮和断面颜色是品质评价最直观的指标。然而，色泽的经验鉴别依赖于人的主观感受，缺乏客观量化指标，传承和应用均存在困难。本文以主产地的野生和栽培乌拉尔甘草为研究对象，采用精密色差仪和视觉分析仪对甘草根皮和断面颜色进行客观的量化分析，采用HPLC对甘草中8种有效成分进行定量分析，将两部分数据采用偏最小二乘回归法进行相关分析，并对相关结果进行比较与验证，探究色泽与有效成分含量的相关性，阐释传统色泽鉴别作为甘草质量评价标准的科学内涵。
1 材料
HP-C220型精密色差仪[汉谱(上海)光电科技有限公司]；VA400型IRIS视觉分析仪[ 阿默思(上海)仪器贸易有限公司]；Agilent 1100型高效液相色谱仪(四元梯度泵、自动进样器、柱温箱和二极管阵列检测器，Agilent科技有限公司)；EYELAN-1100旋转蒸发仪(上海爱朗仪器有限公司)；HHS电热恒温水浴锅(上海博讯实业有限公司医疗设备厂出品)；VO 400真空干燥箱(德国memmert)；DV215CD型电子分析天平(OHAUS CORPORATION，USA)；KQ-500E型超声波清洗器(昆山市超声仪器有限公司)；电加热套(上海一科仪器有限公司)。, 百拇医药(马婷婷 龚慕辛 王智民 贺蕊 吴莎 张村 李静 李朝霞 陆允 许永崧)