HPLC法测定清胰利胆颗粒中丹皮酚的含量
第1页 |
参见附件。
摘要:
目的:建立HPLC法测定清胰利胆颗粒中丹皮酚的分析方法。方法:采用高效液相色谱法。色谱柱Agilent TC-C18(4.6×250mm,5μm),流动相为乙腈-0.1%磷酸溶液(30:70),流速为1mL·min-1,柱温为30℃;检测波长:284nm。进样量:10μL。结果:方法学考察表明,丹皮酚进样量在 0.02278~0.3417 μg范围内具有良好的线性关系(r=0.9999),平均回收率为 100.24%,RSD为 0.59 %(n =7)。 结论:该方法准确、简便、快速,可用于清胰利胆颗粒中丹皮酚的含量测定方法。
关键词:丹皮酚; HPLC; 含量测定
Content determination of Paeonol qingyilidan particles by HPLC
Li Hongxia (Guangxi Hechi Institute for Food and Drug Control Guangxi Hechi 547000)
Abstract:
Objective: To analysis method for determination of Paeonol qingyilidan particles in HPLC method. Methods: The chromatographic column was Agilent TC-C18(4.6×250mm,5μ m ), mobile phase was -0.1% phosphoric acid solution ( 30:70 ), the flow rate was 1mL·min-1, column temperature was 30 ℃; the detection wavelength: 284nm ......
您现在查看是摘要介绍页,详见PDF附件。