双黄连颗粒中绿原酸含量的HPLC测定
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【摘要】 目的 采用高效液相色谱法测定双黄连颗粒中绿原酸的含量。方法 用外标一点法，Diamonsil ODS1 C18色谱柱，甲醇-水-冰醋酸(15∶85∶1)为流动相，流速为1.0 mL/min，λ=324 nm。结果 绿原酸在0.060～1.210 μg范围内呈良好线性，回归方程为Y=105 427X+586.43，r2=0.999 5，平均加样回收率为99.3%，RSD=0.82%(n=6)。结论 本方法简便、准确，专属性强，测定结果重复性好，为双黄连颗粒中绿原酸的定量分析提供了科学有效的方法。
【关键词】 双黄连颗粒；绿原酸；高效液相色谱法；含量测定
Abstract：Objective To establish the method for detemining the content of chlorogenic acid in Shuanghuanglian Keli by HPLC. Methods Using Diamonsil ODS1 C18 column， with methanol-water-glacial acetic acid (15∶85∶1) as the mobile phase， detection wavelength as 324 nm and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.060～1.210 μg for chlorogenic acid and the equation was Y=105 427X+586.43， r2=0.999 5. The average recovery was 99.3% and RSD was 0.82% (n=6). Conclusion This method was simple ......
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