丹参多酚酸盐对大鼠脑缺血再灌注过氧化损伤的保护作用(1)

http://www.100md.com 2010年12月1日 王强 张一 李璐 董博 杨伊林

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     【摘要】 目的 观察丹参多酚酸盐对大鼠局灶性脑缺血再灌注损伤后脑组织内SOD、GSHPx和MDA含量的影响，探讨其缺血脑保护的作用机制。

    方法线栓法制作大鼠中动脉缺血再灌注损伤模型。SD大鼠随机分为正常对照组、缺血再灌注组、丹参多酚酸盐低剂量治疗组(10 mg/kg)和丹参多酚酸盐高剂量治疗组(30 mg/kg)。各组动物按要求给药，在预定时间进行神经损伤行为学评分、脑梗死体积及脑组织内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)活性和丙二醛(MDA)含量的测定。结果 与缺血再灌注组相比，丹参多酚酸盐治疗组大鼠神经损伤行为学评分及脑梗死体积减少，脑组织内SOD、GSHPx活性升高，MDA含量下降，均具有统计学意义(P<0.05)，且呈剂量依赖性。结论丹参多酚酸盐可通过抑制脂质过氧化反应，保护抗氧化酶的活性，从而介导神经保护作用。

    【关键词】 丹参多酚酸盐；脑缺血再灌注；超氧化物歧化酶；谷胱甘肽过氧化物酶；丙二醛

    文章编号：1003-1383(2010)06-0665-03 中图分类号：R 743文献标识码：A

    doi:10.3969/j.issn.1003-1383.2010.06.005

    The effect of salvianolate on the peroxidation injury induced by focal

    cerebral ischemia reperfusion in rats

    WANG Qiang1，ZHANG Yi1，LI Lu2，DONG Bo1，YANG Yilin1

    (1.Department of Neurosurgery，2.Department of Clinical Laboratory，Changzhou First People's Hospital，Changzhou 213003，China)

    【Abstract】 ObjectiveTo study the protective effect of salvianolate on the peroxidation injury induced by focal cerebral ischemia reperfusion in rats and to reveal the mechanism.

    Methods 48 SD rats were divided into four group randomly,they are normal control group，ischemia reperfusion group，salvianolate (10 mg/kg) group and salvianolate(30 mg/kg)group.Each group contained 12 rats.The Longa method was used to establish the MCAO model.The salvianolate at different dose were injected intraperitoneally at different time point according to the test design. The brain infarct volume by TTC stain and behavior scores by Bederson's methods were also determined in order to evaluated the protective effect.Also we use assay kit to determine the activities of SOD and GSHPx，content of MDA in each group.

    Results Comparing with the ischemia reperfusion group, brain infarct volume and behavior score of salvianolate group was lower,the activities of SOD and GSHPx of brain tissue increased,the content of MDA decreased.There was significant difference between them(P<0.05)，the effect of salvianolate was dosedependent.

    Conclusion The salvianolate plays neuroprotective effect by inhibiting the lipid peroxidation and protect the activity of antioxidant enzyme ......

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