【中图分类号】R576；R735.9 【文献标识码】A

    Expression of ANKRD22 and its effects on pancreatic cancer

    SUN Zhijia， SONG Zhuo， LIU Xu， LI Xinji， WANG Yingjie DepartmentofTumorRadiotherapy，PLAAirForceMedicalCenter，Beijing10o142，China Corresponding author： WANG Yingjie， Email： wangyj9999@163.com

    【Abstract】 Objective To explore the expression of ankyrin repeat domain 22 (ANKRD22) in pancreatic cancer (PC)，to analyze its clinical prognosis， and investigate its impact on PC cell phenotypes. Methods The expresson levels of ANKRD22 and their impact on PC prognosis were analyzed using R and Perl languages in the TCGA-PC and GEO databases.ANKRD22-interfered PANC-1 and AsPC-1 celline models were constructed using siRNA. Cell proliferation and invasion capabilities were assessed using CCK-8 assays，colony formation，and Transwell experiments. The regulatory effect of ANKRD22 knockdown on cell apoptosis was evaluated by flow cytometry. Results Compared to normal pancreatic tissues，ANKRD22 expression was significantly elevated in PC tissues.Patients with high ANKRD22 expression had a significantly lower overall survival than those with low expresson ......