【中图分类号】R631；R692.5 【文献标识码】A

    WTAP promotes lipopolysaccharides-induced ferroptosis in human renal tubular epithelial cells by regulating ACSL4 m⁶A methylation

    ZHANG Qin1，TANG Qing2， CHEN Bingyang1

    1.Department of Intensive Care Medicine，Chengdu Seventh People's Hospital，Chengdu 61o213，China 2.Department ofRespiratory Medicine， Chengdu Seventh People's Hospital， Chengdu 610213，China Corresponding author： CHEN Bingyang， Email： bingyang_ch@163.com

    【Abstract】 Objective To investigate the role of Wilms' tumor 1-associating protein (WTAP) in lipopolysaccharide (LPS) induced ferroptosis in human renal tubular epithelial cells (HK-2) and its molecular mechanism. Methods Expression differences of WTAP and long chain acyl-CoA synthetase 4 (ACSL4) between acute kidney injury tissues and healthy control tissues were analyzed based on the GEO database. HK-2 cells were treated with 10μg/mL LPS，and cell viability， cell apoptosis， reactive oxygen species (ROS)， glutathione (GSH) and Fe²⁺ contents were measured. The expression levels of GPX4，ACSL4，and WTAP were evaluated by Western bloting ......