基质金属蛋白酶-9(MMP-9)及其组织抑制剂TIMP-1与肺癌浸润转移及相应CT征象的关系(1)
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[摘要]目的研究基质金属蛋白酶-9(MMP-9)及其组织抑制剂TIMP-1与肺癌浸润转移及CT征象的关系。方法用ELISA法检测血清中MMP-9和TIMP-1的含量，所有病例行胸部CT。结果MMP-9和TIMP-1在小细胞肺癌患者和非小细胞肺癌患者血清中的含量均显著高于正常对照组(P<0.05)。MMP-9和TIMP-1在瘤体>3cm、有纵隔淋巴结转移和存在分叶征者血清中的含量均显著高于瘤体<3cm、无纵隔淋巴结转移和无分叶征者(P<0.05)。结论MMP-9、TIMP-1与肺癌浸润转移关系密切，检测血清中MMP-9和TIMP-1的含量有助于判断肿瘤的恶性度和转移的能力。
关键词：肺癌基质金属蛋白酶CT
The effect ofmatrix metalloproteinase 9 (MMP-9) and its tissue inhibitor (TIMP-1) on invasion or metastasis of lung cancer and the association between them and signs of chest CT
[Abstract] Objective： To study the effect of matrix metalloproteinase 9 and its tissue inhibitor (TIMP-1) on invasion or metastasis of lung cancer and study the association between them and signs with chest CT. Method： Fifty-six specimens were detected by enzyme-linked immunosorbent assay(ELISA)， and chest CT.Results： MMP-9 and TIMP-1 level in lung cancer patients was higher than that in healthy people(P<0.05).And the MMP-9 and TIMP-1 level in lung cancer patients with mass diamitor>3cm was higher than that with diamitor≤3cm， also the same relationship between patients with mass lobe and without mass lobe or with diaphram lymphoid node metastasis and without diaphram lymphoid node metastasis(P<0.05). Conclusion： There is a strong association between MMP-9、TIMP-1 and cancer dissemination. It’s helpful for differentiating m alignant or benign tumor and its metastasis capacity to determine the content of MMP-9 and TIMP-1 in blood serum.
[key words] lung cancer； matrix metalloproteinase； CT
中图分类号：R734.2 文献标识码：B文章编号：1004-7484(2010)12-0057-03
浸润和转移是肿瘤致死的主要原因之一，浸润是肿瘤转移的首要环节。肿瘤细胞必须具有降解细胞外基质的能力，才能向周围浸润，并向血管、淋巴管及远处转移。基质金属蛋白酶(MMP)是一系列在生理情况下可降解细胞外基质及基底膜的内肽酶总称，其中明胶酶(MMP-9)可降解基质中的Ⅳ型、Ⅴ型胶原和明胶，与肿瘤浸润转移关系密切[1\2]。组织金属蛋白抑制剂-1(TIMP-1)为特异性抑制因子，能与MMP-9形成非共价的1：1复合物抑制MMP-9的活性，进而抑制肿瘤的浸润和转移[3]。本研究通过ELASA的方法检测56例肺癌患者血清中MMP-9和TIMP-1的水平，与正常人对照组进行比较，并探讨癌患者血清中MMP-9和TIMP-1的水平与瘤体大小、纵隔淋巴结转移和CT瘤体分叶征之间的关系。
1材料与方法
1.1材料
56例肺癌患者血清标本取自河南大学附属淮河医院胸外科。男性44例，女性12例，年龄38～71岁，中位年龄62岁。其中小细胞肺癌患者21例，非小细胞肺癌患者35例。正常对照为32例健康成人。每例抽取静脉血3ml，血液静置2~5h后，离心2500bp/min 10min，离心后血清位于上层，小心吸取血清于冻存管内 ......
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