壬基酚对P450arom和MIS基因表达的影响(3)
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本研究中,在27℃雄性化水温中,用含雌二醇(E2)饲料喂养比目鱼幼鱼,仍可以完全诱导它们雌性化,并且在同一个生殖腺中P450arom的mRNA表达被诱导而MIS的mRNA表达受到抑制。而NP对27℃水温中幼鱼的雌性化作用的同时也伴随着这一现象,这提示了NP干扰性分化的作用机制很可能与雌激素一样,先通过调节P450arom和MIS mRNA的表达而发挥雌性化作用。这也提示了P450arom和MIS两个基因可以作为鱼类接触壬基酚的早期反应预警标志物,尽早预防它对人体尤其婴幼儿生殖发育系统的危害。今后可以在NP对P450arom和MIS两个基因的转录调节途径分子机制方面作更深入的研究。
4 参考文献
[1]Couse JF, Korach KS. Estrogen receptor null mice:what have we learned and where will they lead us? [J].Endocr Rev,1999,20: 358-417.
[2]Aida T. On the inheritance of colour in a freshwater fish, Aplocheilus latipes Temminck and Schlegel, with special reference to sex-linked inheritance[J]. Genetics,1921,6: 554-573.
[3]Yokota H, Seki M, Maeda M, et al. Life-cycle toxicity of 4-nonylphenol to medaka (Oryzias latipes)[J]. Environ Toxicol Chem,2001,20: 2552-2560
[4]Tabata K. Induction of gynogenetic diploid males and presumption of sex determination mechanisms in the hirame Paralichthys olivaceus[J]. Bull Jap Soc Sci Fish,1991,57: 845-850.
[5]Kitano T, Takamune K, Kobayashi T, et al. Suppression of P450 aromatase gene expression in sex-reversed males produced by rearing genetically female larvae at a high water temperature during a period of sex differentiation in the Japanese flounder (Paralichthys olivaceus)[J]. J Mol Endocrinol,1999, 23: 167-176.
[6]Kitano T, Takamune K, Nagahama Y, et al. Aromatase inhibitor and 17α-methyltestosterone cause sex-reversal from genetical females to phenotypic males and suppression of P450 aromatase gene expression in Japanese flounder (Paralichthys olivaceus)[J] ......
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