氯沙坦拮抗急性胰腺炎胰腺纤维化启动的研究(1)
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【中图分类号】R969.4【文献标识码】A【文章编号】1005-2720(2009)08-0281-04
【摘要】目的：应用免疫组化、RT-PCR等测定方法从组织细胞、基因等水平探讨AT-Ⅱ受体拮抗剂对急性胰腺炎大鼠纤维化启动的作用及意义。方法：采用SHOP法诱导急性胰腺炎大鼠模型，治疗组大鼠给予氯沙坦灌胃，时间从术前3d始，至时间点止，对照组给予生理盐水灌胃。分别于72h、120h处死大鼠，取完整胰腺组织用免疫组化方法检测Ⅰ型胶原和α-SMA的表达，再以逆转录聚合酶链反应(RT-PCR)检测TGF-β1 mRNA、Coll-Ⅰ mRNA的表达情况，并根据结果统计治疗组和对照组差异。结果：光镜下比较可见两组均出现典型急性胰腺炎组织表现，但两组对比无明显可见差异。免疫组化显示对照组和治疗组α-SMA染色无比较差异，治疗组较对照组染色无明显减轻；Coll-Ⅰ分布范围基本同α-SMA，经统计学处理亦无明显差异出现。RT-PCR结果显示，TGF-β1、Coll-Ⅰ的mRNA表达均较正常组明显增强，可见干预组TGF-β mRNA表达量较对照组有所减少，而Coll-Ⅰ的mRNA在72h组变化不明显，而于120h组则略有下降。结论：RAS系统在急性胰腺炎中活性明显增强，和胰腺纤维化之间关系紧密。AT-Ⅱ受体抑制剂能够抑制胰腺内TGF-β mRNA的表达，并最终在一定成度上影响ECM生成。RAS系统是影响胰腺纤维间质组织生成的所有因素中的一个重要部分，单纯抑制RAS系统虽然不能完全阻止胰腺纤维化的产生，但在某种程度上能够延缓纤维化的进展。
【关键词】急性胰腺炎；胰腺纤维化；Ⅰ型胶原；α-平滑肌激动蛋白；转化生长因子-β1
Study of losarton in suppressing pancreatic fibrosis.Wu Fugang1，Zhang Zhigao2，Sun Ziqin2，et al.(1Zhangying hospital of Yuncheng county，Shandong 274700 China；2General hospital of Jinan military reagion of PLA)
【Abstract】Objective：The study is to investigate the effect of losarton，a AT-Ⅱ receptor antagonist，on the start of pancreatic fibrosis in the early time of acute pancretitis by determining the expression of transforming growth factor-β1(TGF-β1)and Collagen Ⅰ messenger RNA and deposition of Collagen-Ⅰ、α-smooth muscle actin(α-SMA)in acute pancreatitis.Methods：Acute pancreatitis was induced in rats by SHOP for 72h and 120h.Treatment group animals are gavaged with losarton 3 days before the induction of pancreatitis，and control animals only with saline.Entire pancreas is harvested in the timepoint of 72h and 120h.Imunohistochemistry was performed with collagen-Ⅰ and α-smooth muscle actin antibodies.Messenger RNA of transforming growth factor-β1(TGF-β1)and Collagen-Ⅰ was detected by reverse transcriptive polymerase chain reaction(RT-PCR) ......
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