HPLC法测定复方心脑宁胶囊中葛根素的含量
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【中图分类号】R92【文献标识码】A【文章编号】1005-2720(2010)06 - 09 - 02
【摘要】目的 建立复方心脑宁胶囊中葛根素的含量测定方法。方法 HPLC法,Waters Nova-pak C18(3.9×150mm,5μm);流动相:乙腈-水(8:92);检测波长:250nm;流速:0.8mL•min–1。结果 色谱分离良好,在78.4～588ng范围内呈线性关系, Y=4.104X-7.734,r=0.9999,平均回收率为102.44%,RSD为2.05%。结论 本测定方法简便可行、重复性好,可用于本制剂中葛根素的含量测定。
【关键词】复方心脑宁胶囊;HPLC法;葛根素;含量测定
The determination of puerarin in Compound Xinnao’ning Capsules by HPLC
FANG Ji-Ming
(The No.1 people’s hospital of Yueyang,Hunan Yueyang414000)
【Abstract】Objective To establish a method for determination ofpuerarin in Compound Xinnaoning capsules. Methods A Waters Nova-pak C18 column (3.9×150mm,5μm)was used, with a mobile phase of acetonitrile-water(8:92).Detective wavelength was 250nm.The flow rate was 0 ......
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