香青兰总黄酮对支气管哮喘大鼠血管内皮生长因子和细胞间黏附分子—1的影响(1)
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摘要：目的 观察香青兰总黄酮对支气管哮喘大鼠血管内皮生长因子(VEGF)、细胞间黏附分子-1(ICAM-1)、一氧化氮(NO)和内皮素-1(ET-1)水平的影响，探讨其作用机制。方法 SD大鼠60只随机分为空白对照组、模型组、地塞米松组(1 mg/kg)及香青兰总黄酮低(90 mg/kg)、中(180 mg/kg)、高(360 mg/kg)剂量组。除空白对照组外，其余各组采用卵白蛋白(OVA)致敏和激发建立大鼠哮喘模型。模型建立后，空白对照组和模型组予以双蒸水灌胃，各给药组灌胃香青兰总黄酮和地塞米松，1次/d，连续30 d。未次给药后，酶联免疫吸附法(ELISA)检测大鼠肺组织VEGF水平及支气管肺泡灌流液(BALF)中ICAM-1含量；放射免疫法和硝酸还原酶法测定BALF中ET-1和NO含量。结果 香青兰总黄酮180、360 mg/kg可降低支气管哮喘大鼠肺组织VEGF水平及BALF中ICAM-1、ET-1和NO含量(P<0.05，P<0.01)。结论 香青兰总黄酮对支气管哮喘大鼠VEGF、ICAM-1、ET-1和NO异常升高具有一定调节作用。
关键词：香青兰总黄酮；支气管哮喘；血管内皮生长因子；细胞间黏附分子-1；一氧化氮；内皮素-1；大鼠
DOI：10.3969/j.issn.1005-5304.2013.09.012
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2013)09-0034-03
Effects of Moldavica Total Flavone on Vascular Endothelial Growth Factor， Intercellular Adhesinon Molecule-1 and Other Cytokines in Bronchial Asthmatic Rats KANG Xiao-long1， HE Cheng-hui2， XING Jian-guo2， YAN Li-li3 (1.Hospital of Chinese Medicine Affiliated to Xinjiang Medical University， Urumqi 830000， China；2.Xinjiang Medicine Research Institute， Urumqi 830004， China；3.School of Pharmacy， Shihezi University， Shihezi 832000， China)
Abstract：Objective To investigate the effects of moldavica total flavone on vascular endothelial growth factor (VEGF)， intercellular adhesion molecule-1 (ICAM-1)， nitric oxide (NO) and endothelin-1 (ET-1) in ovalbumin-induced bronchial asthmatic rats， and explore its mechanism. Methods A total of 60 SD rats was randomly divided into control group， model group， dexamethasone (1 mg/kg) group and moldavica total flavone low (90 mg/kg)， medium (180 mg/kg) and high (360 mg/kg) dose group. Rats asthma model was established by ovalbumin challenge methods except the control group. After modeling， rats in control and model groups were intragastrically given distilled water， rats in medicine groups were intragastrically given moldavica total flavone and dexamethasone for 30 d. VEGF in lung tissue and ICAM-1 in bronchoalveolar lavage fluid (BALF) were detected respectively by ELISA. The content of ET-1 and NO in BALF were analyzed with radioimmunity method and nitric acid reductase method. Results The VEGF in lung tissue and ICAM-1， NO and ET-1 in BALF were decreased by moldavica total flavone at 180 and 360 mg/kg (P<0.05， P<0.01) in ovalbumin-induced bronchial asthmatic rats. Conclusion Moldavica total flavone is effective in adjusting abnormal increase of VEGF， ICAM-1， ET-1 and NO of bronchial asthma rats.
Key words： moldavica total flavone；bronchial asthma；vascular endothelial growth factor；intercellular adhesion molecule-1；nitric oxide；endothelin-1；rats
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