HPLC测定贯叶金丝桃滴丸中绵马贯众素含量
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摘要：目的 建立贯叶金丝桃滴丸中绵马贯众素含量测定的高效液相色谱方法。方法 采用Kromasil-C18 (150 mm×4.6 mm，5 μm)色谱柱，流动相为乙腈-三氯甲烷-异丙醇-0.5%磷酸(52∶8∶30∶10)，流速1.0 mL/min，检测波长285 nm，柱温25 ℃。结果 绵马贯众素进样量在0.236～2.124 μg范围内线性关系良好(r=0.9999)。绵马贯众素的平均加样回收率为99.64%(RSD=1.90%)。结论 该方法简便、准确，适用于贯叶金丝桃滴丸中绵马贯众素的测定。
关键词：贯叶金丝桃滴丸；绵马贯众素；高效液相色谱法
Abstract：Objective To develop an assay of HPLC for the content determination of dryocrassine in Guanye Jinsitao drop pills. Methods The column was Kromasil-C18 (150 mm×4.6 mm， 5 μm)；mobile phase was acetonitrile-chloroform-isopropanol-0.1% phosphoric acid (52∶8∶30∶10)；flow rate was 1.0 mL/min；UV detection was performed at 285 nm；detected drift tube temperature was set at 25 ℃. Results The calibration curves were linear in the range of 0.236-2.124 μg for dryocrassine (r=0.9999) ......
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