甘露消毒丹及其拆方对感染肠道病毒71型细胞miR—146a和Toll样受体4 mRNA表达的影响(1)
摘要：目的 探討甘露消毒丹及其拆方对肠道病毒71型(EV71)感染后免疫及炎症反应的调节作用。方法 运用细胞培养技术，设甘露消毒丹组(甘全方组)、清残方组、利残方组、病毒唑组、正常组、模型组，进行病毒毒力和药物细胞毒性测定后，以药物作用于EV71感染后的RD细胞，24 h后RT-PCR检测各组细胞miR-146a、Toll样受体4(TLR4)mRNA表达。结果 与正常组比较，模型组miR-146a mRNA表达降低，TLR4 mRNA表达升高；与模型组比较，甘全方组、清残方组、利残方组miR-146a mRNA表达升高、TLR4 mRNA表达降低，病毒唑组与模型组比较差异不明显；与甘全方组比较，清残方组miR-146a、TLR4 mRNA表达降低，利残方组miR-146a mRNA表达升高、TLR4 mRNA表达降低；与清残方组比较，利残方组miR-146a mRNA表达升高，TLR4 mRNA表达无明显差异。结论 甘露消毒丹可能通过调节miR-146a、TLR4 mRNA表达从而影响EV71感染所致免疫反应，其中清残方与利残方可能存在互相拮抗效应。
关键词：甘露消毒丹；拆方；肠道病毒71型；miRNA-146a；Toll样受体4
DOI：10.3969/j.issn.1005-5304.2017.03.015
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2017)03-0061-05
Effects of Ganlu Xiaodu Dan and Its Incomplete Prescription on Expressions of MiR-146a and TLR4 mRNA in Cells Infected by EV71 AI Bi-chen1， HE Yi-rong1， CAO Rong1， HE Dong2， ZHAO Guo-rong1， HE You-shun1 (1. TCM School of Hunan University of Chinese Medicine， Changsha 410208， China； 2. Graduate School of Hunan University of Chinese Medicine， Changsha 410208， China)
Abstract： Objective To observe the effects of Ganlu Xiaodu Dan and its incomplete prescription on expressions of MiR-146a and TLR4 mRNA in RD cells infected by EV71. Methods With technique of cell culturing， Ganlu Xiaodu Dan therapy group， incomplete Qing prescription therapy group， incomplete Li prescription therapy group， normal cells control group， model control group and ribavirin control group were set， and tests for virus toxicity and medicine toxicity in cells were taken， then expressions of miRNA-146a and TLR4 mRNA in these RD cells 24 hours after intervention with medicine were detected. Results Compared with normal cells control group， miR-146a in mRNA model control group decreased and TLR4 mRNA increased. Compared with model control group， miR-146a mRNA in Ganlu Xiaodu Dan therapy group， incomplete Qing prescription therapy group and incomplete Li prescription therapy group all increased while TLR4 mRNA decreased， and differences between ribavirin control group and model control group were not significant. Compared with Ganlu Xiaodu Dan therapy group， both expressions of miR-146a and TLR4 mRNA in incomplete Qing prescription therapy group were lower； miR-146a increased and TLR4 mRNA decreased in incomplete Li prescription therapy group. Compared with incomplete Qing prescription therapy group， miR-146a mRNA in incomplete Li prescription therapy group increased， but expression of TLR4mRNA between them was not significant. Conclusion Ganlu Xiaodu Dan can regulate the immune reactions caused by infection of EV71 by increasing expression of miR-146a mRNA and reducing expression of TLR4 mRNA. There may be antagonism effect between incomplete Qing prescription and incomplete Li prescription., 百拇医药(艾碧琛 何宜荣 曹蓉 何栋 赵国荣 贺又舜)
关键词：甘露消毒丹；拆方；肠道病毒71型；miRNA-146a；Toll样受体4
DOI：10.3969/j.issn.1005-5304.2017.03.015
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2017)03-0061-05
Effects of Ganlu Xiaodu Dan and Its Incomplete Prescription on Expressions of MiR-146a and TLR4 mRNA in Cells Infected by EV71 AI Bi-chen1， HE Yi-rong1， CAO Rong1， HE Dong2， ZHAO Guo-rong1， HE You-shun1 (1. TCM School of Hunan University of Chinese Medicine， Changsha 410208， China； 2. Graduate School of Hunan University of Chinese Medicine， Changsha 410208， China)
Abstract： Objective To observe the effects of Ganlu Xiaodu Dan and its incomplete prescription on expressions of MiR-146a and TLR4 mRNA in RD cells infected by EV71. Methods With technique of cell culturing， Ganlu Xiaodu Dan therapy group， incomplete Qing prescription therapy group， incomplete Li prescription therapy group， normal cells control group， model control group and ribavirin control group were set， and tests for virus toxicity and medicine toxicity in cells were taken， then expressions of miRNA-146a and TLR4 mRNA in these RD cells 24 hours after intervention with medicine were detected. Results Compared with normal cells control group， miR-146a in mRNA model control group decreased and TLR4 mRNA increased. Compared with model control group， miR-146a mRNA in Ganlu Xiaodu Dan therapy group， incomplete Qing prescription therapy group and incomplete Li prescription therapy group all increased while TLR4 mRNA decreased， and differences between ribavirin control group and model control group were not significant. Compared with Ganlu Xiaodu Dan therapy group， both expressions of miR-146a and TLR4 mRNA in incomplete Qing prescription therapy group were lower； miR-146a increased and TLR4 mRNA decreased in incomplete Li prescription therapy group. Compared with incomplete Qing prescription therapy group， miR-146a mRNA in incomplete Li prescription therapy group increased， but expression of TLR4mRNA between them was not significant. Conclusion Ganlu Xiaodu Dan can regulate the immune reactions caused by infection of EV71 by increasing expression of miR-146a mRNA and reducing expression of TLR4 mRNA. There may be antagonism effect between incomplete Qing prescription and incomplete Li prescription., 百拇医药(艾碧琛 何宜荣 曹蓉 何栋 赵国荣 贺又舜)