不同干燥条件连翘叶茶有效成分含量比较(1)
摘要：目的 比較不同干燥条件下连翘叶茶中总黄酮、连翘酯苷A和连翘苷含量，确定连翘叶茶最佳干燥方式。方法 采用紫外分光光度法，以亚硝酸钠-三氯化铝-氢氧化钠溶液体系为显色剂，在波长500 nm处测定总黄酮含量；采用HPLC同时测定连翘酯苷A和连翘苷含量，色谱柱为Diamosil C18(2)柱(4.6 mm×250 mm，5 ?m)，流动相为乙腈-0.4%乙酸水溶液，梯度洗脱，检测波长277 nm，柱温30 ℃，流速1.0 mL/min。结果 不同干燥条件连翘叶茶中总黄酮含量基本相同；不同干燥条件连翘叶茶中连翘酯苷A和连翘苷含量顺序为：通风阴干干燥>真空干燥>鼓风干燥。结论 不同干燥条件对连翘叶茶中总黄酮含量无明显影响，对连翘酯苷A和连翘苷的含量影响较大，通风阴干比鼓风干燥和真空干燥更有利于连翘酯苷A和连翘苷成分的保存。
关键词：连翘叶茶；总黄酮；连翘酯苷A；连翘苷；紫外分光光度法；高效液相色谱法
DOI：10.3969/j.issn.1005-5304.2017.08.017
中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2017)08-0076-04
Comparison of Effects of Different Drying Methods on Contents of Effective Components of Forsythiae Fructus Leaves Tea MA Jing-mei1，2， WANG Ying-chun1，2， LI Chen1，2， WANG Meng1，2， ZHENG Qian3， NIU Li-ying1，2 (1. Hebei University of Chinese Medicine， Shijiazhuang 050091， China； 2. Hebei TCM Formula Granule Engineering&Technology Research Center， Shijiazhuang 050091， China； 3. Shijiazhuang Traditional Chinese Medicine Hospital， Shijiazhuang 050001， China)
Abstract： Objective To compare the contents of total flavonoids， forsythoside A and phillyrin in Forsythiae Fructus leaves tea under different drying conditions； To determine the best drying method for Forsythiae Fructus leaves tea. Methods With the sodium nitrite-aluminum trichloride-sodium hydroxide solution as color reagent， total flavonoids in forsythiae Fructus leaves tea were determined by UV spectrophotometry at the wavelength of 500 nm. HPLC was used to determine the contents of forsythiaside A and phillyrin. The analysis was performed on a Diamosil C18 (2) column (4.6 mm × 250 mm， 5 ?m)； the mobile phase was composed of acetonitrile and 0.4% acetic acid with gradient elution； the detection wavelength was set at 277 nm； the flow rate was 1.0 mL/min at column temperature of 30 ℃. Results The content of total flavonoids of Forsythiae Fructus leaves tea under different drying conditions was basically the same； the sequence of the contents of forsythoside A and Forsythin of Forsythiae Fructus leaves tea under different drying conditions was： ventilated drying > vacuum drying > blast drying. Conclusion Different drying conditions have no effect on the contents of total flavonoids in Forsythiae Fructus leaves tea， but have obvious effects on the contents of forsythiaside A and phillyrin. Ventilation shade is better than blast drying and vacuum drying for preverence of forsythiaside A and forsythin.
Key words： Forsythiae Fructus leaves tea； total flavonoids； forsythoside A； phillyrin； UVspectrophotometry； HPLC, 百拇医药(麻景梅 王迎春 李琛 王梦 郑倩 牛丽颖)
关键词：连翘叶茶；总黄酮；连翘酯苷A；连翘苷；紫外分光光度法；高效液相色谱法
DOI：10.3969/j.issn.1005-5304.2017.08.017
中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2017)08-0076-04
Comparison of Effects of Different Drying Methods on Contents of Effective Components of Forsythiae Fructus Leaves Tea MA Jing-mei1，2， WANG Ying-chun1，2， LI Chen1，2， WANG Meng1，2， ZHENG Qian3， NIU Li-ying1，2 (1. Hebei University of Chinese Medicine， Shijiazhuang 050091， China； 2. Hebei TCM Formula Granule Engineering&Technology Research Center， Shijiazhuang 050091， China； 3. Shijiazhuang Traditional Chinese Medicine Hospital， Shijiazhuang 050001， China)
Abstract： Objective To compare the contents of total flavonoids， forsythoside A and phillyrin in Forsythiae Fructus leaves tea under different drying conditions； To determine the best drying method for Forsythiae Fructus leaves tea. Methods With the sodium nitrite-aluminum trichloride-sodium hydroxide solution as color reagent， total flavonoids in forsythiae Fructus leaves tea were determined by UV spectrophotometry at the wavelength of 500 nm. HPLC was used to determine the contents of forsythiaside A and phillyrin. The analysis was performed on a Diamosil C18 (2) column (4.6 mm × 250 mm， 5 ?m)； the mobile phase was composed of acetonitrile and 0.4% acetic acid with gradient elution； the detection wavelength was set at 277 nm； the flow rate was 1.0 mL/min at column temperature of 30 ℃. Results The content of total flavonoids of Forsythiae Fructus leaves tea under different drying conditions was basically the same； the sequence of the contents of forsythoside A and Forsythin of Forsythiae Fructus leaves tea under different drying conditions was： ventilated drying > vacuum drying > blast drying. Conclusion Different drying conditions have no effect on the contents of total flavonoids in Forsythiae Fructus leaves tea， but have obvious effects on the contents of forsythiaside A and phillyrin. Ventilation shade is better than blast drying and vacuum drying for preverence of forsythiaside A and forsythin.
Key words： Forsythiae Fructus leaves tea； total flavonoids； forsythoside A； phillyrin； UVspectrophotometry； HPLC, 百拇医药(麻景梅 王迎春 李琛 王梦 郑倩 牛丽颖)