百事乐加味方对卒中后抑郁大鼠海马神经元自噬蛋白的影响(1)
摘要：目的 觀察百事乐加味方对卒中后抑郁(PSD)大鼠神经功能缺损评分、形态学改变及海马自噬蛋白mTOR、Beclin-1、LC-3Ⅱ表达的影响，探讨PSD神经元细胞自噬机制及中药防治作用。方法 采用大脑中动脉栓塞+慢性不可预见温和应激+孤养法制作PSD模型，分组前进行行为学评分，随机分为假手术组、抑郁组、卒中组、PSD组、阳性药组、百事乐加味方组，各给药组给予相应药物，连续28 d。HE、Nissl染色观察大鼠海马形态;免疫组化检测PSD大鼠海马mTOR、Beclin-1、LC-3Ⅱ的表达。结果 与假手术组比较，PSD组大鼠神经功能缺损评分明显升高，海马mTOR表达明显降低，Beclin-1、LC-3Ⅱ表达明显升高(P<0.01);与PSD组比较，百事乐加味方组大鼠功能缺损评分显著降低，海马组织病理形态明显改善，海马mTOR蛋白表达明显升高，Beclin-1、LC-3Ⅱ蛋白表达明显降低(P<0.05，P<0.01)。结论 百事乐加味方可有效改善PSD大鼠行为学、病理学表现，抑制神经细胞异常自噬可能是其抗PSD的重要机制之一。
关键词：百事乐加味方;卒中后抑郁;海马;自噬;脑缺血;大鼠
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2019)02-0052-05
DOI：10.3969/j.issn.1005-5304.2019.02.012
开放科学(资源服务)标识码(OSID)：
Abstract： ObjectiveTo observe the effects of modified Baishile Formula on the neurological deficit score and the expressions of mTOR， Beclin-1 and LC-3Ⅱ in hippocampus of post-stroke depression (PSD) rats; To explore the preventive and therapeutic mechanism of TCM for post-stroke depression from neuronal autophagy. MethodsThe rat model of focal cerebral ischemia was established by the method of middle cerebral artery occlusion (MCAO)， and the PSD model was established by combining with chronic unpredictable mild stress (CUMS) and solitary nourishment. The behavioral scores were evaluated by Longa grading method before dividing the groups. The rats were randomly divided into sham-operation group， depression group， post-stroke group， PSD group， positive medicine group and modified Baishile Formula group. Each administration group was given relevant medicine for 28 d. The morphological changes of hippocampus were observed by HE staining and Nissl staining. Immunohistochemical method was used to detect the expressions of mTOR， Beclin-1 and LC-3Ⅱ in hippocampus of PSD rats. ResultsCompared with the sham-operation group， the neurological deficit score of the PSD group significantly increased， the expression of mTOR in the hippocampus significantly decreased， and the expressions of Beclin-1 and LC-3Ⅱ significantly increased (P<0.01). Compared with the PSD group， the neurological deficit score of the rats in modified Baishile Formula groupsignificantly decreased， the pathological morphology of the hippocampus wassignificantly improved， the expression of mTOR protein in the hippocampus significantly increased， and the expression of Beclin-1 and LC-3Ⅱ proteins significantly decreased (P<0.05， P<0.01). ConclusionModified Baishile Formula can effectively improve the behavioral and pathological symptoms of PSD rats and inhibit autonomic phagocytosis. It may be one of the important mechanisms of treating PSD., 百拇医药(刘羽 刘检 易亚乔 凌佳 刘丽 邵乐 刘林)
关键词：百事乐加味方;卒中后抑郁;海马;自噬;脑缺血;大鼠
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2019)02-0052-05
DOI：10.3969/j.issn.1005-5304.2019.02.012
开放科学(资源服务)标识码(OSID)：
Abstract： ObjectiveTo observe the effects of modified Baishile Formula on the neurological deficit score and the expressions of mTOR， Beclin-1 and LC-3Ⅱ in hippocampus of post-stroke depression (PSD) rats; To explore the preventive and therapeutic mechanism of TCM for post-stroke depression from neuronal autophagy. MethodsThe rat model of focal cerebral ischemia was established by the method of middle cerebral artery occlusion (MCAO)， and the PSD model was established by combining with chronic unpredictable mild stress (CUMS) and solitary nourishment. The behavioral scores were evaluated by Longa grading method before dividing the groups. The rats were randomly divided into sham-operation group， depression group， post-stroke group， PSD group， positive medicine group and modified Baishile Formula group. Each administration group was given relevant medicine for 28 d. The morphological changes of hippocampus were observed by HE staining and Nissl staining. Immunohistochemical method was used to detect the expressions of mTOR， Beclin-1 and LC-3Ⅱ in hippocampus of PSD rats. ResultsCompared with the sham-operation group， the neurological deficit score of the PSD group significantly increased， the expression of mTOR in the hippocampus significantly decreased， and the expressions of Beclin-1 and LC-3Ⅱ significantly increased (P<0.01). Compared with the PSD group， the neurological deficit score of the rats in modified Baishile Formula groupsignificantly decreased， the pathological morphology of the hippocampus wassignificantly improved， the expression of mTOR protein in the hippocampus significantly increased， and the expression of Beclin-1 and LC-3Ⅱ proteins significantly decreased (P<0.05， P<0.01). ConclusionModified Baishile Formula can effectively improve the behavioral and pathological symptoms of PSD rats and inhibit autonomic phagocytosis. It may be one of the important mechanisms of treating PSD., 百拇医药(刘羽 刘检 易亚乔 凌佳 刘丽 邵乐 刘林)