HPLC测定莲不同部位紫云英苷及异鼠李素-3-O-葡萄糖苷含

HPLC测定莲不同部位紫云英苷及异鼠李素-3-O-葡萄糖苷含量(1)

http://www.100md.com 2019年12月1日《中国中医药信息杂志》 2019年第12期

     摘要：目的建立莲不同部位中紫云英苷及异鼠李素-3-O-葡萄糖苷含量的HPLC测定方法。方法采用Diamonsil C₁₈色谱柱(200 mm×4.6 mm，5 μm)，以乙腈-0.5%甲酸溶液为流动相，梯度洗脱，检测波长347 nm，流速0.8 mL/min，柱温30 ℃，进样量10 μL。结果在上述色谱条件下，紫云英苷、异鼠李素-3-O-葡萄糖苷与相邻色谱峰的分离度良好。紫云英苷在0.100 5～2.01 μg范圍内有良好的线性关系(r=0.999 6)，平均加样回收率为100.96%(RSD=2.68%);异鼠李素-3-O-葡萄糖苷在0.026～0.52 μg范围内有良好的线性关系(r=0.999 4)，平均加样回收率为98.91%(RSD=2.34%)。结论本研究建立的方法操作简便，结果可靠，可为莲相关药材的质量控制及资源开发提供依据。

    关键词：莲;紫云英苷;异鼠李素-3-O-葡萄糖苷;高效液相色谱法;含量测定
, 百拇医药
    中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2019)12-0075-04

    DOI：10.3969/j.issn.1005-5304.2019.12.016 开放科学(资源服务)标识码(OSID)：

Content Determination of Astragalin and Isorhamnetin-3-O-glucoside inNelumbo NuciferaGaertn. by HPLC

PEI Xiangping¹， LI Huifeng¹， DU Chenhui¹， LIU Haixia²， ZHANG Shurong¹

1. Shanxi University of Chinese Medicine， Jinzhong 030600， China; 2. Shanxi Zhendong Ante Biological Pharmaceutical Co.， Ltd.， Jinzhong 030600， China

Abstract： Objective To establish an HPLC method for content determination of astragalin and isorhamnetin-3-O-glucoside in Nelumbo nucifera Gaertn. Methods The chromatographic separation was performed on a Diamonsil C₁₈ column (200 mm × 4.6 mm， 5 μm); the column temperature was maintained at 30 ℃; the mobile phase was acetonitrile-0.5% formic acid solution as gradient elution; the flow rate was 0.8 mL/min; the detection wavelength was at 347 nm; the injection volume was 10 μL. Results Under above chromatographic conditions， astragalin and isorhamnetin-3-O-glucoside with adjacent other peaks were among the good separation. Astragalin had a good linear relationship in the range of 0.1005–2.01 μg (r=0.999 6); the average recovery was 100.96% (RSD=2.68%). Isorhamnetin-3-O-glucoside had a good linear relationship in the range of 0.026–0.52 μg (r=0.999 3); the average recovery was 98.57% (RSD=2.34%). Conclusion The method is convenient and reliable， which can provide evidence for the quality control and resource development of Nelumbo nucifera Gaertn. as a related medicine., 百拇医药(裴香萍李慧峰杜晨晖刘海霞张淑蓉)

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