关键词:百合;傅里叶变换衰减全反射红外光谱;超高效液相色谱-二极管阵列检测器;王百合苷A
, 百拇医药
中图分类号:R284.1 文献标识码:A 文章编号:1005-5304(2020)01-0057-06
DOI:10.3969/j.issn.1005-5304.201901219
Identification of Lilii Bulbus and Its Counterfeit by ATR-FTIR and UPLC-DAD
YUAN Zhiying1,2,3, LI Dongyang1, LI Yalin1, GUAN He1, LIU Xiangdan1, XIE Mengzhou1,3, HUANG Huiyong1,3
1. Hunan University of Chinese Medicine, Changsha 410208, China; 2. Hunan Engineering Technology Center of Standardization and Function of Chinese Herbal Decoction Pieces, Changsha 410208, China; 3. Hunan Engineering Technology Center of Functional Food Homology of Medicine, Changsha 410208, China
, 百拇医药
Abstract: Objective To quickly distinguish different kinds of Lilii Bulbus powder based on ATR-FTIR and UPLC-DAD. Methods The infrared spectral data of samples were collected by ATR-FTIR, and the data was imported into Simca software to establish the orthogonal partial least squares discriminant analysis (OPLS-DA) model. On this basis, UPLC-DAD was used to determine the content of regaloside A in genuine Lilii Bulbus and Lilium trompeten (counterfeit) and microwave dried Lilium lancifolium Thunb., and the differences among different samples were analyzed. Results Two-dimensional score map of OPLS-DA and hierarchical cluster analysis were used to classify the three groups of Lilium lancifolium Thunb. and Lilium broumii F.E. Brown var.viridulum Baker, Lilium lancifolium Thunb. and Lilium trompeten (counterfeit), hot-air dried Lilium lancifolium Thunb. and microwave dried Lilium lancifolium Thunb. The content of regaloside A in Lilium lancifolium Thunb. was 2.09–3.28 mg/g, 1.36–1.77 mg/g in Lilium broumii F.E. Brown var.viridulum Baker, 1.03–1.05 mg/g in Lilium trompeten (counterfeit), and 3.92–4.01 mg/g in microwave Lilium lancifolium Thunb. The content of regaloside A in Lilium lancifolium Thunb. and Lilium broumii, http://www.100md.com(袁志鹰 李东洋 李亚林 管贺 刘湘丹 谢梦洲 黄惠勇)
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