Isolation and Identification of Endophytic Fungi in Roots， Stems and Leaves of Dendrobium officinale

LUO Xiaofang1， XU Shujuan2， CHEN Lihua3， WANG Dongmei1， ZHANG Lu1， FEI Xiaotong2， ZHAO Zitong2

1. Center of Experiment， Northwest Minzu University， Lanzhou 730030， China;

2. College of Life Science and Engineering， Northwest Minzu University， Lanzhou 730030， China;

3. School of Chemical Engineering， Northwest Minzu University， Lanzhou 730030， China

    Abstract： Objective To isolate and identify the endophytic fungi in different tissues of Dendrobium officinale; To provide basis for development and utilization of Dendrobium officinale. Methods The endophytic fungi was isolated from the roots， stems and leaves of Dendrobium officinale by using various basal culture media. The isolated strains were purified by the plate streaking， and their colony morphology was observed. Endophytic fungi were identified by morphological， physiological and biochemical molecular characteristics and molecular biological methods. Results Seven strains of endophytic fungi were isolated. The quantity and species of microorganisms differed in roots， stems and leaves. Only X1 existed in roots， stems and leaves at the same time. Three strains of fungi with stable characters were identified by molecular biology. By molecular bioassay of three fungi with stable traits， XJ1 had 99% homology with the 16S rDNA sequence of Alternaria sp.; XY6 had 93% homology with the 16S rDNA sequence of Alternaria sp.; X1 strain had 99% homology with the 16S rDNA sequence of Neurospora africana. Conclusion This study isolates and identifies endophytic fungi from the roots， stems and leaves of Dendrobium officinale， which can provide a basis for the development and utilization of Dendrobium officinale resources.(雒晓芳 许淑娟 陈丽华 王冬梅 张璐 费孝桐 赵梓彤)