大鼠PDGF—C基因真核表达载体的构建与鉴定
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摘要：目的 构建并鉴定大鼠血小板衍生生长因子C(PDGF-C)基因的真核表达载体。方法 根据GenBank数据库提供的基因序列，设计合成大鼠PDGF-C基因CDS扩增引物，RT-PCR法获得目的基因，T载体连接测序后，构建pEGFP-N1-PDGF-C真核表达载体，双酶切鉴定。结果 RT-PCR 扩增大鼠PDGF-C基因片段为1053bp，与预期大小相符；测序结果表明该目的基因序列与GenBank 公布序列一致；构建的重组质粒pEGFP-N1-PDGF-C双酶切鉴定正确。结论 成功构建了大鼠PDGF-C基因的真核表达载体。
关键词：大鼠；PDGF-C基因；载体构建
Abstract：Objective To construct and identify a eukaryotic expression plasmid of rat PDGF-C gene. Methods An rat PDGF-C gene fragment was amplified and sequenced， then cloned into pEGFP-N1 to construct the eukaryotic expression plasmid pEGFP-N1-PDGF-C. The recombinant plasmid was identified by using double restriction enzyme digestion. Results The RT-PCR product was a gene fragment with 1053bp， and the nucleotide sequence of that was consistent with the sequence of the rat PDGF-C gene registered in GenBank ......
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