摘要：目的 探讨Vasohibin-1在人结肠癌细胞株SW480和人脐静脉内皮细胞(HUVECs)共培养体系中对HUVECs的作用。方法 通过四甲基偶氮唑蓝评价Vasohibin-1对HUVECs的抑制作用；以流式细胞术测定HUVECs凋亡率。结果 Vasohibin-1能抑制人结肠癌细胞株SW480和HUVECs共培养体系中HUVECs增殖；流式细胞术测定显示随其浓度的增加HUVECs的凋亡率在逐渐增加。结论 Vasohibin-1能促进人结肠癌细胞株SW480与HUVECs共培养中HUVECs的凋亡。

    关键词：血管生成抑制因子-1；血管内皮细胞；凋亡

    Abstract：Objective To study the mechanism of Vasohibin-1 on promoting the apoptosis of human umbilical vein endothelial cells(HUVEC) in colonic cancer cell line SW480 co-culture system. Methods Tetrazolium blue was used for the evaluation on proliferation inhibition of Vasohibin-1 in HUVECs；flow cytometry was used to determine the apoptosis rate.Results Vasohibin-1 can significantly inhibit HUVECs proliferation and promote its apoptosis in SW480 co-cultured system.Conclusion Vasohibin-1 can promote HUVECs apoptosis in colonic cancer cell co-cultured system.

    Key words：Vasohibin-1；Vascular endothelial cells； Apoptosis

    Vasohibin-1是近年来发现的一个血管内皮生长因子-A(vascular endothelial growth factor A ......