干细胞标志Nanog在食管鳞癌组织中表达及预后关系(1)
摘要：目的 探讨食管鳞癌组织中干细胞标志Nanog表达及其与肿瘤病理特征和预后的关系。方法 采用免疫组化方法测定60例食管鳞癌组织和10例癌旁组织中干细胞标志Nanog的表达情况，比较它在组织中的表达差异，及其与食管癌相关临床病理特征和预后的关系。结果 在60例患者中，26例出现复发转移，Nanog在转移组中表达率分别为61.5%(16/26)，在非转移组中分别为26.5%(9/34)，差异有统计学意义，P值<0.05。食管鳞癌原发灶分化程度不同，Nanog表达存在差异，但是同T和N分期没有明显的相关性(P>0.05)。Nanog为阳性表达其转移率显著高于为阴性表达者。结论 食管鳞癌组织中Nanog 表达与患者术后复发转移有一定关系，该检测有助于预后的评价。
关键词：食管鳞癌；Nanog
Expression and Clinical Significances of Nanog in Esophagea Squamous Carcinoma
, http://www.100md.com
MA Cheng-fang1， YU Zheng2， LI Shao-peng1， LI Zhi-hua1， WEI Chun-yong1， WANG Li-xu1， HONG Qiong-chuan1
(1.Department of Cardiothoracic Surgery， Longgang District Central Hospital of Shenzhen，Shenzhen 518116， Guangdong，China；
2. Department of Physical Examination， People Hospital of Shenzhen Longgang District，Shenzhen 518116， Guangdong，China)
Abstract：Objective To investigate the expression of Nanog in esophageal squamous carcinoma and their correlations with the pathologic parameter of cancer and prognosis.Methods The expression of Nanog in 60 surgical specimens of esophageal squamous carcinoma and 10 paracarcinoma tissues was detected by immunohistochemistry method. The correlations between the expression of Nanog and the clinical pathological characteristics were analyzed statistically.Results Relapse and metastasis of 26 cases was detected in all patients. In metastasis group， the positive expression rate of Nanog was 61.5 %(16/26)). And the positive expression rate of Nanog in non-metastasis group was 26.5%(9/34).The difference was signigicant ( P < 0. 05). Expression of Nanog signigicantly correlated with differentiation， but was not associated with T stage or N stage . Metastatic rate in positive expression was higher than in negative expression.Conclusion The expression of Nanog related with progression and prognosis of esophageal squamous carcinoma， they are both hopeful predictors for patients with esophageal squamous carcinoma.
, 百拇医药
Key words：Esophageal squamous carcinoma；Nanog
食管癌是我國高发肿瘤之一，食管鳞癌术后的复发转移是目前临床常见的现象，总体治疗效果不佳，如何有效的评估预测是目前国内外研究的一个热点。近年出现的干细胞理论证实与肿瘤的发生、侵润和转移密切相关，迄今已在多种恶性肿瘤中鉴定出具有干细胞性质的恶性肿瘤细胞。作为全能或多能性干细胞的标志，Nanog基因是维系干细胞自我更新的关键性基因。本研究采用免疫组化方法对食管鳞癌组织中干细胞标志Nanog表达情况进行分析，探讨其与食管鳞癌临床特征及预后的关系。
1 资料与方法
1.1一般资料 选取2011年1月～2012年12月在我院胸外科住院治疗并确诊的食管鳞癌患者60例，其中男性32例，女性28例。年龄38～71岁，中位年龄为49岁。根据食管鳞癌TNM分期(AJCC2009)标准，ⅡA期28例，ⅡB期11例，ⅢA期21例。26(43.3%)例患者确诊复发转移，其中9例为吻合口复发，10例为单个脏器转移，7例为多发脏器或淋巴结转移。所有患者术前未行放化疗，术前检查及术中探查均未发现明确远处转移。术后行辅助PF或TP方案化疗4～6个周期。随访时仍生存37例，已死亡23例。, http://www.100md.com(麻成方 余征 李绍鹏 李志华 魏春勇 汪礼旭 洪琼川)
关键词：食管鳞癌；Nanog
Expression and Clinical Significances of Nanog in Esophagea Squamous Carcinoma
, http://www.100md.com
MA Cheng-fang1， YU Zheng2， LI Shao-peng1， LI Zhi-hua1， WEI Chun-yong1， WANG Li-xu1， HONG Qiong-chuan1
(1.Department of Cardiothoracic Surgery， Longgang District Central Hospital of Shenzhen，Shenzhen 518116， Guangdong，China；
2. Department of Physical Examination， People Hospital of Shenzhen Longgang District，Shenzhen 518116， Guangdong，China)
Abstract：Objective To investigate the expression of Nanog in esophageal squamous carcinoma and their correlations with the pathologic parameter of cancer and prognosis.Methods The expression of Nanog in 60 surgical specimens of esophageal squamous carcinoma and 10 paracarcinoma tissues was detected by immunohistochemistry method. The correlations between the expression of Nanog and the clinical pathological characteristics were analyzed statistically.Results Relapse and metastasis of 26 cases was detected in all patients. In metastasis group， the positive expression rate of Nanog was 61.5 %(16/26)). And the positive expression rate of Nanog in non-metastasis group was 26.5%(9/34).The difference was signigicant ( P < 0. 05). Expression of Nanog signigicantly correlated with differentiation， but was not associated with T stage or N stage . Metastatic rate in positive expression was higher than in negative expression.Conclusion The expression of Nanog related with progression and prognosis of esophageal squamous carcinoma， they are both hopeful predictors for patients with esophageal squamous carcinoma.
, 百拇医药
Key words：Esophageal squamous carcinoma；Nanog
食管癌是我國高发肿瘤之一，食管鳞癌术后的复发转移是目前临床常见的现象，总体治疗效果不佳，如何有效的评估预测是目前国内外研究的一个热点。近年出现的干细胞理论证实与肿瘤的发生、侵润和转移密切相关，迄今已在多种恶性肿瘤中鉴定出具有干细胞性质的恶性肿瘤细胞。作为全能或多能性干细胞的标志，Nanog基因是维系干细胞自我更新的关键性基因。本研究采用免疫组化方法对食管鳞癌组织中干细胞标志Nanog表达情况进行分析，探讨其与食管鳞癌临床特征及预后的关系。
1 资料与方法
1.1一般资料 选取2011年1月～2012年12月在我院胸外科住院治疗并确诊的食管鳞癌患者60例，其中男性32例，女性28例。年龄38～71岁，中位年龄为49岁。根据食管鳞癌TNM分期(AJCC2009)标准，ⅡA期28例，ⅡB期11例，ⅢA期21例。26(43.3%)例患者确诊复发转移，其中9例为吻合口复发，10例为单个脏器转移，7例为多发脏器或淋巴结转移。所有患者术前未行放化疗，术前检查及术中探查均未发现明确远处转移。术后行辅助PF或TP方案化疗4～6个周期。随访时仍生存37例，已死亡23例。, http://www.100md.com(麻成方 余征 李绍鹏 李志华 魏春勇 汪礼旭 洪琼川)