HSP90—eNOS在糖尿病大鼠胸主动脉的表达及普罗布考干预实验(1)
摘要：目的 建立糖尿病大血管病變模型，观察HSP90和eNOS在糖尿病大鼠胸主动脉中的表达，以及普罗布考干预治疗后的影响。方法 50只雄性SD大鼠分为正常对照组(N组)和模型组，模型组以高糖高脂喂养联合腹腔注射STZ成模。以随机血糖≥16.7 mmol/L并维持2 w者定为造模成功。成模大鼠随机分为糖尿病组(DM组)和普罗布考干预组(PB组)，PB组每日以普罗布考灌胃，N组和DM组以等体积柠檬酸钠缓冲液灌胃。8 w后处死大鼠并分离胸主动脉，光镜下观察胸主动脉病变，免疫组织化学检测血管组织HSP90、eNOS的表达。结果 ①DM组大鼠胸主动脉内皮细胞广泛脱落，平滑肌排列紊乱。PB组大鼠胸主动脉可见内皮细胞灶状脱落，平滑肌排列稍紊乱。N组大鼠内皮细胞光滑平整，平滑肌排列整齐。②DM组大鼠较N组大鼠相比，胸主动脉表达HSP90明显升高(P<0.05)。PB组与DM组比较，胸主动脉HSP90表达无明显差异(P>0.05)。三组间胸主动脉表达eNOS无明显差异(P>0.05)。结论 ①高糖高脂喂养联合腹腔注射STZ的糖尿病大鼠，胸主动脉呈早期动脉粥样硬化改变；②HSP90在糖尿病大鼠胸主动脉表达明显升高，普罗布考干预治疗后对其表达无明显影响。
, 百拇医药
关键词：HSP90；ENOS；糖尿病鼠；糖尿病大血管病；普罗布考
Expression of HSP90-eNOS in Thoracic Aorta of Diabetic Rats and Probucol Intervention
HUANG De-bin，ZHAI Xin，LI Xiao-xing
(Department of Endocrinology and Metabolism，Third Hospital of Changsha City，Changsha 410015，Hunan，China)
Abstract：Objective To establish diabetic model，observe the expression of HSP90 and eNOS in thoracic aorta in diabetic rats，and the effects of probucol treatment.Methods 50 male SD rats were divided into normal control group(N group)and model group，model group with high fat and high sugar feeding combined with intraperitoneal injection of STZ model.With random blood glucose≥16.7 mmol/L and maintained for 2 w as animal model.The rats were randomly divided into diabetic group(DM group)and probucol group(PB group)，PB group daily with probucol gavage，N group and DM group with equal volume of citric acid sodium buffer intragastric.8 w rats were killed after separation and thoracic aorta，thoracic aortic lesions observed under light microscope，detection of vascular tissue by immunohistochemistry.The rats were sacrificed 8 w after separation and thoracic aorta，thoracic aortic lesions observed under light microscope，immunohistochemical detection of vascular tissue HSP90，eNOS expression. Results ①thoracic aorta endothelial cells in DM group rats off，smooth muscle derangement thoracic aorta of.PB rats showed focal loss of endothelial cells，smooth muscle cells arranged a little disorder of endothelial cells of rats in.N group is smooth，smooth muscle cells arranged in neat rows②the rats in the DM group than in N group rats compared to the expression of HSP90 in thoracic aorta(P<0.05)increased significantly in.PB group compared with DM group，the expression of HSP90 in thoracic aorta(P>0.05).No significant difference between the three groups had no significant difference in eNOS expression in the aorta of diabetic rats(P>0.05).Conclusion ①the high-fat feeding combined with intraperitoneal injection of STZ，thoracic aorta showed early atherosclerotic changes；②the expression of HSP90 in the chest the aorta of diabetic rats increased significantly，probucol treatment had no obvious effect on its expression., 百拇医药(黄德斌 翟鑫 李晓行)
, 百拇医药
关键词：HSP90；ENOS；糖尿病鼠；糖尿病大血管病；普罗布考
Expression of HSP90-eNOS in Thoracic Aorta of Diabetic Rats and Probucol Intervention
HUANG De-bin，ZHAI Xin，LI Xiao-xing
(Department of Endocrinology and Metabolism，Third Hospital of Changsha City，Changsha 410015，Hunan，China)
Abstract：Objective To establish diabetic model，observe the expression of HSP90 and eNOS in thoracic aorta in diabetic rats，and the effects of probucol treatment.Methods 50 male SD rats were divided into normal control group(N group)and model group，model group with high fat and high sugar feeding combined with intraperitoneal injection of STZ model.With random blood glucose≥16.7 mmol/L and maintained for 2 w as animal model.The rats were randomly divided into diabetic group(DM group)and probucol group(PB group)，PB group daily with probucol gavage，N group and DM group with equal volume of citric acid sodium buffer intragastric.8 w rats were killed after separation and thoracic aorta，thoracic aortic lesions observed under light microscope，detection of vascular tissue by immunohistochemistry.The rats were sacrificed 8 w after separation and thoracic aorta，thoracic aortic lesions observed under light microscope，immunohistochemical detection of vascular tissue HSP90，eNOS expression. Results ①thoracic aorta endothelial cells in DM group rats off，smooth muscle derangement thoracic aorta of.PB rats showed focal loss of endothelial cells，smooth muscle cells arranged a little disorder of endothelial cells of rats in.N group is smooth，smooth muscle cells arranged in neat rows②the rats in the DM group than in N group rats compared to the expression of HSP90 in thoracic aorta(P<0.05)increased significantly in.PB group compared with DM group，the expression of HSP90 in thoracic aorta(P>0.05).No significant difference between the three groups had no significant difference in eNOS expression in the aorta of diabetic rats(P>0.05).Conclusion ①the high-fat feeding combined with intraperitoneal injection of STZ，thoracic aorta showed early atherosclerotic changes；②the expression of HSP90 in the chest the aorta of diabetic rats increased significantly，probucol treatment had no obvious effect on its expression., 百拇医药(黄德斌 翟鑫 李晓行)
参见：首页 > 医疗版 > 疾病专题 > 内分泌科 > 糖尿病