基于LC—MS技术毛细支气管炎风热闭肺证的代谢组学研究(1)
摘要：目的 采用高效液相色谱-质谱联用技术的代谢组学方法，探析毛细支气管炎中医风热闭肺证在血清内源性代谢物的变化，以寻找毛细支气管炎中医风热闭肺证的诊断标志物。方法 收集毛细支气管炎患儿风热闭肺证(风热闭肺组)20例和健康儿童(对照组)20例。以液相色谱-质谱联用技术对两组受试患儿的血清样本进行代谢图谱分析，并对潜在的生物标记物进行鉴定。结果 风热闭肺组与正常对照组相比很多离子峰高、峰面积均存在着明显差异，筛出具有统计学差异的差异生物标志物共8种：L-缬氨酸，尿素，L-天门冬氨酸，2氨-咪唑，D-甘露糖，L-天门冬酰胺，D-果糖，L-谷氨酰胺。结论 本研究从代谢组学小分子水平初步反映了小儿毛细支气管炎风热闭肺证的代谢特征，为从生物代谢的角度研究中医证候的现代科学内涵提供了一定的实验依据。
关键词：代谢组学；毛细支气管炎；风热闭肺证；液相色谱-质谱联用技术
Study on Metabonomics of Bronchiolitis with Wind Heat Obstructing Lung Syndrome Based on LC-MS
, http://www.100md.com
CHEN Chao，BIAN Xun
(Department of Pediatrics，Nanjing Traditional Chinese Medicine and Western Medicine Hospital，Nanjing University of Traditional Chinese Medicine，Nanjing 210014，Jiangsu，China)
Abstract：Objective Using high performance liquid chromatography-mass spectrometry metabonomic technique study method，the change of capillary bronchitis chinese wind heat closed the lung in serum endogenous metabolites，to find the diagnosis of bronchiolitis in Traditional Chinese Medicine of wind heat closed lungs markers.Methods Capillary bronchitis in children with wind heat closed the lung(wind heat closed lung group)and 20 healthy children(control group)20 cases.The two groups of serum sample test were analyzed by using metabolic profiling by liquid chromatography mass spectrometry，and to identify potential biomarkers.Results Many group of wind heat lung ion peak high compared with the normal control group，area there were significant differences， with statistical difference between screening biomarkers were 8： L-valine， urea， L-aspartic acid， 2 of ammonia - imidazole，D-mannose，L-asparagine，D-fructose，L-glutamine.Conclusion This study from the metabonomics small molecular level preliminary reflect the metabolic characteristics of children with bronchiolitis in wind heat closed the lung，and provide a certain experimental basis for the modern scientific connotation of TCM syndromes from the perspective of biological metabolism.
, 百拇医药
Key words：Metabonomics；Bronchiolitis；Wind heat obstructing lung syndrome；Liquid chromatography-mass spectrometry
中醫证候代谢组学研究[1]是运用代谢组学相关技术对中医不同证候患者或模型动物的血浆、尿液等样本进行代谢产物分析，得到各自的代谢产物图谱，筛查出特异性代谢产物的标志物，并用生物信息学方法分析生物标志物的功能，以确定“证相关代谢谱群”。本研究采用高效液相色谱-质谱联用代谢组学技术对毛细支气管炎风热闭肺证患儿血清内源性小分子代谢物的变化差异进行分析，探析毛细支气管炎中医风热闭肺证的代谢组学特征，以期为毛细支气管炎中医证候学研究提供代谢组学依据。
1 资料与方法
1.1一般资料 全部病例均来源于2012年2月～2014年4月我院儿科RSV毛细支气管炎风热闭肺证住院患儿，共20例。2014年3月～2015年10月健康体检患儿20例。两组在性别、年龄方面无统计学差异(P>0.05)，签署知情同意书。
1.2病例纳入标准 西医诊断参照全国高等学校教材《儿科学》(第七版)，中医疾病诊断、证候诊断参照普通高等教育“十五”国家级规划教材、新世纪全国高等中医药院校规划教材《中医儿科学· 肺系疾病·肺炎喘嗽》及国家技术监督局发布《中华人民共和国国家标准·中医临床诊疗术语证候部分》的相关内容。排除应激和先天性心脏病、支气管肺发育不良、早产及免疫缺陷等患儿。, 百拇医药(陈超 边逊)
关键词：代谢组学；毛细支气管炎；风热闭肺证；液相色谱-质谱联用技术
Study on Metabonomics of Bronchiolitis with Wind Heat Obstructing Lung Syndrome Based on LC-MS
, http://www.100md.com
CHEN Chao，BIAN Xun
(Department of Pediatrics，Nanjing Traditional Chinese Medicine and Western Medicine Hospital，Nanjing University of Traditional Chinese Medicine，Nanjing 210014，Jiangsu，China)
Abstract：Objective Using high performance liquid chromatography-mass spectrometry metabonomic technique study method，the change of capillary bronchitis chinese wind heat closed the lung in serum endogenous metabolites，to find the diagnosis of bronchiolitis in Traditional Chinese Medicine of wind heat closed lungs markers.Methods Capillary bronchitis in children with wind heat closed the lung(wind heat closed lung group)and 20 healthy children(control group)20 cases.The two groups of serum sample test were analyzed by using metabolic profiling by liquid chromatography mass spectrometry，and to identify potential biomarkers.Results Many group of wind heat lung ion peak high compared with the normal control group，area there were significant differences， with statistical difference between screening biomarkers were 8： L-valine， urea， L-aspartic acid， 2 of ammonia - imidazole，D-mannose，L-asparagine，D-fructose，L-glutamine.Conclusion This study from the metabonomics small molecular level preliminary reflect the metabolic characteristics of children with bronchiolitis in wind heat closed the lung，and provide a certain experimental basis for the modern scientific connotation of TCM syndromes from the perspective of biological metabolism.
, 百拇医药
Key words：Metabonomics；Bronchiolitis；Wind heat obstructing lung syndrome；Liquid chromatography-mass spectrometry
中醫证候代谢组学研究[1]是运用代谢组学相关技术对中医不同证候患者或模型动物的血浆、尿液等样本进行代谢产物分析，得到各自的代谢产物图谱，筛查出特异性代谢产物的标志物，并用生物信息学方法分析生物标志物的功能，以确定“证相关代谢谱群”。本研究采用高效液相色谱-质谱联用代谢组学技术对毛细支气管炎风热闭肺证患儿血清内源性小分子代谢物的变化差异进行分析，探析毛细支气管炎中医风热闭肺证的代谢组学特征，以期为毛细支气管炎中医证候学研究提供代谢组学依据。
1 资料与方法
1.1一般资料 全部病例均来源于2012年2月～2014年4月我院儿科RSV毛细支气管炎风热闭肺证住院患儿，共20例。2014年3月～2015年10月健康体检患儿20例。两组在性别、年龄方面无统计学差异(P>0.05)，签署知情同意书。
1.2病例纳入标准 西医诊断参照全国高等学校教材《儿科学》(第七版)，中医疾病诊断、证候诊断参照普通高等教育“十五”国家级规划教材、新世纪全国高等中医药院校规划教材《中医儿科学· 肺系疾病·肺炎喘嗽》及国家技术监督局发布《中华人民共和国国家标准·中医临床诊疗术语证候部分》的相关内容。排除应激和先天性心脏病、支气管肺发育不良、早产及免疫缺陷等患儿。, 百拇医药(陈超 边逊)