大鼠阴道上皮细胞体外培养研究进展(3)
综上所述，现有体外培养大鼠阴道上皮细胞方法各有优缺点，组织块培养法为经典且基础的培养法，其操作简单，成功率高，且成本较低，但培养周期长；酶消化法培养周期短，可在短时间内获得大量纯度较高的细胞用于实验研究，优于组织法，使用较为广泛，但除了胰酶、胶原酶和中性蛋白酶应用比较成熟外，其它酶类很少深入研讨。培养基成分尚待进一步完善，以模拟细胞体内生长环境，为更深层次的研究提供基础。对于动情周期的选取与阴道上皮细胞培养的关系目前研究极少，仍需进一步研究探讨其意义。培养瓶包被技术的应用一定程度上解决了细胞贴壁困难的问题。
参考文献：
[1]Zhang Jie-Yu，Liu Jin-Hui，Liu Fa-Di，et al.Vulvovaginal candidiasis：species distribution，fluconazole resistance and drug efflux pump gene overexpression[J].Mycoses，2014，57(10)：584.
, 百拇医药
[2]Rathod S D，Buffler P A.Highly-cited estimates of the cumulative incidence and recurrence of vulvovaginal candidiasis are inadequately documented[J].Bmc Womens Health，2014，14(1)：1-4.
[3]李雅釵，黄向华，张明乐.聚乳酸-聚羟基乙酸/Ⅰ型胶原复合支架的生物相容性研究[J].中国修复重建外科杂志，2015(9)：1144-1149.
[4]李亚楠.阴道上皮细胞体外诱导骨髓间充质干细胞向上皮细胞分化的研究[D].河北医科大学，2013.
[5]刘方方，李亚楠，黄向华.大鼠阴道黏膜上皮细胞原代分离及培养方法的改进[J].现代妇产科进展，2013，22(6)：465-468.
, http://www.100md.com
[6]Haseeb M A，Agrawal R，Fried B.Reduced [14C]-methionine uptake and fecundity in Schistosoma mansoni females treated with recombinant tumor necrosis factor α in vitro[J].Acta Parasitologica，2017，62(1)：164-170.
[7]Li X，Liu X，Yu S.Psychological Stress-Derived Prolactin Modulates Occludin Expression in Vaginal Epithelial Cells to Compromise Barrier Function[J].Cell Physiol Biochem，2015，37(1)：153-161.
[8]Liu Y Z，Wang T T，Zhang Y Z.A modified method for the culture of naturally HPV-infected high-grade cervical intraepithelial neoplasia keratinocytes from human neoplastic cervical biopsies[J].Oncology Letters，2016，11(2)：1457-1462.
, http://www.100md.com
[9]Loureiro R R，Cristovam P C，Martins C M，et al.Comparison of culture media for ex vivo cultivation of limbal epithelial progenitor cells[J].Molecular Vision，2013，19(1)：69-77.
[10]Li Y，Liu F，Zhang Z，et al.Bone marrow mesenchymal stem cells could acquire the phenotypes of epithelial cells and accelerate vaginal reconstruction combined with small intestinal submucosa[J].Cell Biology International，2015，39(11)：1225-1233.
[11]Bikle D D，Xie Z，Tu C L.Calcium regulation of keratinocyte differentiation[J].Expert Review of Endocrinology& Metabolism，2012，7(4)：461-472.编辑/成森, http://www.100md.com(卢伟 祁文瑾)
参考文献：
[1]Zhang Jie-Yu，Liu Jin-Hui，Liu Fa-Di，et al.Vulvovaginal candidiasis：species distribution，fluconazole resistance and drug efflux pump gene overexpression[J].Mycoses，2014，57(10)：584.
, 百拇医药
[2]Rathod S D，Buffler P A.Highly-cited estimates of the cumulative incidence and recurrence of vulvovaginal candidiasis are inadequately documented[J].Bmc Womens Health，2014，14(1)：1-4.
[3]李雅釵，黄向华，张明乐.聚乳酸-聚羟基乙酸/Ⅰ型胶原复合支架的生物相容性研究[J].中国修复重建外科杂志，2015(9)：1144-1149.
[4]李亚楠.阴道上皮细胞体外诱导骨髓间充质干细胞向上皮细胞分化的研究[D].河北医科大学，2013.
[5]刘方方，李亚楠，黄向华.大鼠阴道黏膜上皮细胞原代分离及培养方法的改进[J].现代妇产科进展，2013，22(6)：465-468.
, http://www.100md.com
[6]Haseeb M A，Agrawal R，Fried B.Reduced [14C]-methionine uptake and fecundity in Schistosoma mansoni females treated with recombinant tumor necrosis factor α in vitro[J].Acta Parasitologica，2017，62(1)：164-170.
[7]Li X，Liu X，Yu S.Psychological Stress-Derived Prolactin Modulates Occludin Expression in Vaginal Epithelial Cells to Compromise Barrier Function[J].Cell Physiol Biochem，2015，37(1)：153-161.
[8]Liu Y Z，Wang T T，Zhang Y Z.A modified method for the culture of naturally HPV-infected high-grade cervical intraepithelial neoplasia keratinocytes from human neoplastic cervical biopsies[J].Oncology Letters，2016，11(2)：1457-1462.
, http://www.100md.com
[9]Loureiro R R，Cristovam P C，Martins C M，et al.Comparison of culture media for ex vivo cultivation of limbal epithelial progenitor cells[J].Molecular Vision，2013，19(1)：69-77.
[10]Li Y，Liu F，Zhang Z，et al.Bone marrow mesenchymal stem cells could acquire the phenotypes of epithelial cells and accelerate vaginal reconstruction combined with small intestinal submucosa[J].Cell Biology International，2015，39(11)：1225-1233.
[11]Bikle D D，Xie Z，Tu C L.Calcium regulation of keratinocyte differentiation[J].Expert Review of Endocrinology& Metabolism，2012，7(4)：461-472.编辑/成森, http://www.100md.com(卢伟 祁文瑾)