不同雷公藤制剂中6个成分的含量研究(1)
摘 要:目的 建立測定雷公藤制剂中的6种成分(雷公藤甲素、雷公藤内酯酮、雷酚内酯、雷公藤次碱、雷公藤红素及雷公藤内酯甲)的高效液相色谱方法。方法 采用Agilent TC-C18柱(250 mm×4.6 mm,5 μm),以10 mmol/L乙酸铵水溶液-乙腈为流动相梯度洗脱,流速为0.75 ml/min,检测波长为225 nm,柱温为25 ℃。结果 6种成分的进样浓度为0.1~156.25 μg/ml范围内与峰面积积分值线性关系良好,平均回收率为98.5%(RSD=1.9%)、99.2%(RSD=1.9%)、99.5%(RSD=1.5%)、98.7%(RSD=1.2%)、98.8%(RSD=1.6%)、99.0%(RSD=1.7%)。结论 所建立的测定方法操作方便,重复性强,结果准确,可用于雷公藤及其相关产品的质量分析;不同雷公藤制剂中6个成分的含量存在差异。
关键词:雷公藤制剂;雷公藤化学成分;反相高效液相色谱法
中图分类号:R284.1 文献标识码:A DOI:10.3969/j.issn.1006-1959.2018.05.023
, http://www.100md.com
文章编号:1006-1959(2018)05-0070-04
Study on the Content of Six Components in Different Tripterygium Wilfordii Preparations
QI Ai-rong1,FU Bo1,LIU Xiao-yan1,LI Shun-min1,WEN Shan-shi2,ZHENG Ping3,CHEN Jian-ping3
(1.4th College of Clinical Medicine,Guangzhou University of Traditional Chinese Medicine,Shenzhen 518033,Guangdong,China;
2.Nanao People's Hospital,Dapeng New District,Shenzhen 518121,Guangdong,China;
, 百拇医药
3.Key Laboratory of Traditional Chinese Medicine Preparations,Shenzhen Traditional Chinese Medicine Hospital,Shenzhen 518033,Guangdong,China)
Abstract:Objective To establish a high performance liquid chromatography method for the determination of 6 components in Tripterygium wilfordii(Triptolide,triptolide,lycopolide,Tripterygium,tripterin,and triptolide a).Methods A Agilent TC-C18 column of (250 mm×4.6 mm,5 μm)was used.The mobile phase gradient elution was 10 mmol/L ammonium acetate aqueous solution and acetonitrile.The flow rate was 0.75 ml/min,the detection wavelength was 225 nm,and the column temperature was 25 ℃.Results The injection concentration of the six components was 0.1~156.25 μg/ml and the peak area integral value was linear,the average recovery rate was 98.5%(RSD=1.9%),99.2%(RSD=1.9%),99.5%(RSD=1.5%),98.7%(RSD=1.2%),98.8%(RSD=1.6%),99.0%(RSD=1.7%).Conclusion The established method is convenient, reproducible and accurate.It can be used for the quality analysis of Tripterygium wilfordii and its related products,and the content of 6 components in different Tripterygium wilfordii preparations is different.
Key words:Tripterygium wilfordii preparation;Chemical constituents of Tripterygium wilfordii;Reversed phase high performance liquid chromatography, http://www.100md.com(祁爱蓉 傅博 刘小燕 李顺民 文善适 郑平 陈剑平)
关键词:雷公藤制剂;雷公藤化学成分;反相高效液相色谱法
中图分类号:R284.1 文献标识码:A DOI:10.3969/j.issn.1006-1959.2018.05.023
, http://www.100md.com
文章编号:1006-1959(2018)05-0070-04
Study on the Content of Six Components in Different Tripterygium Wilfordii Preparations
QI Ai-rong1,FU Bo1,LIU Xiao-yan1,LI Shun-min1,WEN Shan-shi2,ZHENG Ping3,CHEN Jian-ping3
(1.4th College of Clinical Medicine,Guangzhou University of Traditional Chinese Medicine,Shenzhen 518033,Guangdong,China;
2.Nanao People's Hospital,Dapeng New District,Shenzhen 518121,Guangdong,China;
, 百拇医药
3.Key Laboratory of Traditional Chinese Medicine Preparations,Shenzhen Traditional Chinese Medicine Hospital,Shenzhen 518033,Guangdong,China)
Abstract:Objective To establish a high performance liquid chromatography method for the determination of 6 components in Tripterygium wilfordii(Triptolide,triptolide,lycopolide,Tripterygium,tripterin,and triptolide a).Methods A Agilent TC-C18 column of (250 mm×4.6 mm,5 μm)was used.The mobile phase gradient elution was 10 mmol/L ammonium acetate aqueous solution and acetonitrile.The flow rate was 0.75 ml/min,the detection wavelength was 225 nm,and the column temperature was 25 ℃.Results The injection concentration of the six components was 0.1~156.25 μg/ml and the peak area integral value was linear,the average recovery rate was 98.5%(RSD=1.9%),99.2%(RSD=1.9%),99.5%(RSD=1.5%),98.7%(RSD=1.2%),98.8%(RSD=1.6%),99.0%(RSD=1.7%).Conclusion The established method is convenient, reproducible and accurate.It can be used for the quality analysis of Tripterygium wilfordii and its related products,and the content of 6 components in different Tripterygium wilfordii preparations is different.
Key words:Tripterygium wilfordii preparation;Chemical constituents of Tripterygium wilfordii;Reversed phase high performance liquid chromatography, http://www.100md.com(祁爱蓉 傅博 刘小燕 李顺民 文善适 郑平 陈剑平)