AP—4、EZH2基因表达量与子宫内膜癌病灶中细胞凋亡、上皮间质转化的相关性研究(1)
摘 要：目的 研究AP-4、EZH2基因表達量与子宫内膜癌病灶中细胞凋亡、上皮间质转化的相关性。方法 回顾分析2017年1月～2018年1月在我院进行切除术治疗的35例子宫内膜癌患者临床资料，收集子宫内膜癌病灶和癌旁病灶适量，提取RNA后分别测定AP-4、EZH2基因表达量，进一步测定病灶中细胞凋亡、上皮间质转化基因的表达量。结果 子宫内膜癌病灶内AP-4、EZH2基因表达量高于癌旁病灶，差异有统计学意义(P<0.05)；子宫内膜癌病灶内SRPX2、RLIP76、ZEB1、SALL4、TET1、RANKL、N-cadherin的mRNA表达量高于癌旁病灶，Fat-1、ITLN-1、Caspase-3、α-catenin的mRNA表达量低于癌旁病灶；AP-4高表达病灶内SRPX2、RLIP76的mRNA表达量高于低表达病灶，Fat-1、ITLN-1、Caspase-3的mRNA表达量低于AP-4低表达病灶；EZH2高表达病灶内ZEB1、SALL4、TET1、RANKL、N-cadherin的mRNA表达量高于EZH2低表达病灶，α-catenin的mRNA表达量低于EZH2低表达病灶。结论 宫内膜癌病灶内AP-4、EZH2基因高表达量，可以抑制癌细胞凋亡、促进癌细胞上皮间质转化，值得临床予以重视和关注。
, 百拇医药
关键词：AP-4、EZH2基因；子宫内膜癌；细胞凋亡；上皮间质转化
中图分类号：R737.33 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.14.026
文章编号：1006-1959(2018)14-0092-03
Abstract：Objective To investigate the correlation between the expression of AP-4 and EZH2 gene and apoptosis and epithelial-mesenchymal transition in endometrial cancer.Methods The clinical data of 35 patients with endometrial cancer who underwent resection in our hospital from January 2017 to January 2018 were retrospectively analyzed.Endometrial cancer lesions and adjacent lesions were collected.After RNA extraction，AP-4 was determined.The expression level of EZH2 gene was further measured in the lesions and the expression level of epithelial-mesenchymal transition genes.Results The expression of AP-4 and EZH2 in endometrial cancer lesions was higher than that in adjacent lesions，and the difference was statistically significant(P<0.05).The mRNA expression levels of SRPX2，RLIP76，ZEB1，SALL4，TET1，RANKL and N-cadherin in endometrial cancer lesions were higher than those in adjacent lesions，and the mRNA expression levels of Fat-1，ITLN-1，Caspase-3 and α-catenin were higher.The mRNA expression levels of Fat-1，ITLN-1，Caspase-3 and α-catenin were lower than those of adjacent lesions.The mRNA expression of SRPX2 and RLIP76 in AP-4 high expression lesions was higher than that of low expression lesions，Fat-1 and ITLN.-1，Caspase-3 mRNA expression was lower than AP-4 low expression lesion；EZH2 high expression lesions ZEB1，SALL4，TET1，RANKL，N-cadherin mRNA expression was higher than EZH2 low expression lesion，the mRNA expression level of α-catenin was lower than that of EZH2 low expression lesions.Conclusion The high expression levels of AP-4 and EZH2 in endometrial cancer lesions can inhibit cancer cell apoptosis and promote cancer cell epithelial-mesenchymal transition.It is worthy of clinical attention.
Key words：AP-4，EZH2 gene；Endometrial cancer；Apoptosis；Epithelial-mesenchymal transition, 百拇医药(季福水)
, 百拇医药
关键词：AP-4、EZH2基因；子宫内膜癌；细胞凋亡；上皮间质转化
中图分类号：R737.33 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.14.026
文章编号：1006-1959(2018)14-0092-03
Abstract：Objective To investigate the correlation between the expression of AP-4 and EZH2 gene and apoptosis and epithelial-mesenchymal transition in endometrial cancer.Methods The clinical data of 35 patients with endometrial cancer who underwent resection in our hospital from January 2017 to January 2018 were retrospectively analyzed.Endometrial cancer lesions and adjacent lesions were collected.After RNA extraction，AP-4 was determined.The expression level of EZH2 gene was further measured in the lesions and the expression level of epithelial-mesenchymal transition genes.Results The expression of AP-4 and EZH2 in endometrial cancer lesions was higher than that in adjacent lesions，and the difference was statistically significant(P<0.05).The mRNA expression levels of SRPX2，RLIP76，ZEB1，SALL4，TET1，RANKL and N-cadherin in endometrial cancer lesions were higher than those in adjacent lesions，and the mRNA expression levels of Fat-1，ITLN-1，Caspase-3 and α-catenin were higher.The mRNA expression levels of Fat-1，ITLN-1，Caspase-3 and α-catenin were lower than those of adjacent lesions.The mRNA expression of SRPX2 and RLIP76 in AP-4 high expression lesions was higher than that of low expression lesions，Fat-1 and ITLN.-1，Caspase-3 mRNA expression was lower than AP-4 low expression lesion；EZH2 high expression lesions ZEB1，SALL4，TET1，RANKL，N-cadherin mRNA expression was higher than EZH2 low expression lesion，the mRNA expression level of α-catenin was lower than that of EZH2 low expression lesions.Conclusion The high expression levels of AP-4 and EZH2 in endometrial cancer lesions can inhibit cancer cell apoptosis and promote cancer cell epithelial-mesenchymal transition.It is worthy of clinical attention.
Key words：AP-4，EZH2 gene；Endometrial cancer；Apoptosis；Epithelial-mesenchymal transition, 百拇医药(季福水)