2016年我院儿童病院肠道病毒71型感染情况调查(1)
摘 要：目的 分析我院儿童病院肠道病毒71型的感染现状，了解手足口病的流行病学特征。方法 2016年我院采集儿童病院抗EV-71抗体血样共1645 例。运用描述流行病学的方法和Excel软件对上述数据进行回顾性调查分析。结果 2016年儿童病院1645 名研究人群中，抗EV71 IgM的阳性率为 12.52%。其中4岁组抗EV71 IgM的阳性率(3.34%)最高；高发月份为6月份，发病率23.83%。阳性病例男性(51.94%)多于女性(48.06%)。结论 手足口病发病有明显时间、人群分布特点，需重点加强校医及园医培训，提高综合素质和识病、防病能力。
关键词：手足口病；肠道病毒71型；感染情况；流行病学描述
中图分类号：R725.7 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.15.036
文章编号：1006-1959(2018)15-0115-03
, http://www.100md.com
Investigation of Human Enterovirus 71 Infection in our Children's Hospital in 2016
LIU Na，YAN Wei-li
(Department of Laboratory，the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine，Tianjin 300250，China)
Abstract：Objective To analyze the status of Human Enterovirus 71 infection in our children’s hospital and to understand the epidemiological characteristics of hand-foot and mouth disease.Methods 1645 blood samples of anti-EV-71 antibody were collected in our hospital in 2016.The methods of descriptive epidemiology and Excel software were used to carry out retrospective investigation and analysis of the above data.Results The positive rate of anti-EV71 IgM was 12.52% in 1645 cases from children’s hospitals in 2016.The positive rate of anti-EV71 IgM in 4 years old group was the highest(3.34%).The month of the highest incidence was June，and the incidence was 23.83%.Positive cases in male(51.94%)were more than females(48.06%).Conclusion Hand-foot and mouth disease has obvious time and population distribution characteristics，it is necessary to strengthen the training of school doctors and garden doctors to improve the comprehensive quality and ability of disease recognition and prevention.
, 百拇医药
Key words：Hand-foot and mouth disease；Human enterovirus 71；Infection；Epidemiological description
腸道病毒71型(Human enterovirus 71，EV71)是引发手足口病的常见病毒。由于EV71对于中枢神经系统有极高的感染性，故临床上以手足口症及泡疹性咽峡炎最为常见，但是有文献报道[1]少数严重者可出现心肌炎、肺水肿、脑炎等比较严重的损害。人群对EV71普遍易感，以婴幼儿和低龄儿童感染为主。临床症状以手、足皮肤疱疹和口腔黏膜溃疡为主。现收集2016年我院儿童病院就诊的 1645 名婴幼儿和儿童的EV71检查结果分析，报道如下。
1资料与方法
1.1研究对象 回顾天津中医药大学第二附属医院2016年1月～12月的婴幼儿和低龄儿童的抗EV71抗体检查结果，对手足口病的发病特点进行总结，了解我院片区内手足口病的流行特征。
, http://www.100md.com
1.2 方法 试剂肠道病毒71型IgM抗体检测试剂盒(胶体金法)。内含检测卡，样本稀释管和吸管。检测原理利用免疫层析技术采用捕获法检测EV71-IgM抗体。在硝酸纤维膜上包被抗-人IgM(抗μ链)，同时采用胶体金标记EV71抗体及抗原。检测时，如果样品内含有EV71-IgM抗体，将形成“包被抗μ链+EV71-IgM抗体+金标记EV71抗体及抗原”复合物，而在检测区凝集形成一条红色线，在对照区形成另外一条红色线；样品内不含有EV71-IgM抗体时，则只在对照区形成一条红色线，检测区无可见条带。
1.3标本采集及检测方法 采集婴幼儿和儿童的左手无名指末梢血。采血深度约2 mm，稍加挤压以血液能流出为宜，干棉球擦去第一滴血，先取20 μl全血，加入至120 μl稀释液的试管中，等待检测血常规；再取30 μl全血加入至样本稀释管(商品化试剂)中充分混匀。室温下，在检测卡中央孔处加入80μl稀释样本，平置于台面上，30 min内观察结果。, 百拇医药(刘娜 闫卫利)
关键词：手足口病；肠道病毒71型；感染情况；流行病学描述
中图分类号：R725.7 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.15.036
文章编号：1006-1959(2018)15-0115-03
, http://www.100md.com
Investigation of Human Enterovirus 71 Infection in our Children's Hospital in 2016
LIU Na，YAN Wei-li
(Department of Laboratory，the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine，Tianjin 300250，China)
Abstract：Objective To analyze the status of Human Enterovirus 71 infection in our children’s hospital and to understand the epidemiological characteristics of hand-foot and mouth disease.Methods 1645 blood samples of anti-EV-71 antibody were collected in our hospital in 2016.The methods of descriptive epidemiology and Excel software were used to carry out retrospective investigation and analysis of the above data.Results The positive rate of anti-EV71 IgM was 12.52% in 1645 cases from children’s hospitals in 2016.The positive rate of anti-EV71 IgM in 4 years old group was the highest(3.34%).The month of the highest incidence was June，and the incidence was 23.83%.Positive cases in male(51.94%)were more than females(48.06%).Conclusion Hand-foot and mouth disease has obvious time and population distribution characteristics，it is necessary to strengthen the training of school doctors and garden doctors to improve the comprehensive quality and ability of disease recognition and prevention.
, 百拇医药
Key words：Hand-foot and mouth disease；Human enterovirus 71；Infection；Epidemiological description
腸道病毒71型(Human enterovirus 71，EV71)是引发手足口病的常见病毒。由于EV71对于中枢神经系统有极高的感染性，故临床上以手足口症及泡疹性咽峡炎最为常见，但是有文献报道[1]少数严重者可出现心肌炎、肺水肿、脑炎等比较严重的损害。人群对EV71普遍易感，以婴幼儿和低龄儿童感染为主。临床症状以手、足皮肤疱疹和口腔黏膜溃疡为主。现收集2016年我院儿童病院就诊的 1645 名婴幼儿和儿童的EV71检查结果分析，报道如下。
1资料与方法
1.1研究对象 回顾天津中医药大学第二附属医院2016年1月～12月的婴幼儿和低龄儿童的抗EV71抗体检查结果，对手足口病的发病特点进行总结，了解我院片区内手足口病的流行特征。
, http://www.100md.com
1.2 方法 试剂肠道病毒71型IgM抗体检测试剂盒(胶体金法)。内含检测卡，样本稀释管和吸管。检测原理利用免疫层析技术采用捕获法检测EV71-IgM抗体。在硝酸纤维膜上包被抗-人IgM(抗μ链)，同时采用胶体金标记EV71抗体及抗原。检测时，如果样品内含有EV71-IgM抗体，将形成“包被抗μ链+EV71-IgM抗体+金标记EV71抗体及抗原”复合物，而在检测区凝集形成一条红色线，在对照区形成另外一条红色线；样品内不含有EV71-IgM抗体时，则只在对照区形成一条红色线，检测区无可见条带。
1.3标本采集及检测方法 采集婴幼儿和儿童的左手无名指末梢血。采血深度约2 mm，稍加挤压以血液能流出为宜，干棉球擦去第一滴血，先取20 μl全血，加入至120 μl稀释液的试管中，等待检测血常规；再取30 μl全血加入至样本稀释管(商品化试剂)中充分混匀。室温下，在检测卡中央孔处加入80μl稀释样本，平置于台面上，30 min内观察结果。, 百拇医药(刘娜 闫卫利)