不同碘浓度对大鼠甲状腺摄碘功能的影响(1)
摘 要：目的 观察不同碘摄入量对大鼠促甲状腺激素受体(TSHR)mRNA、钠碘转运体(NIS)mRNA以及血清过氧化物酶抗体(TPOAb)表达水平的影响。方法 SD大鼠30只，随机分成低碘组(LI)、适碘组(NI)和高碘组(HI)，每组10只。于实验第8、16周分批处死。实时荧光定量聚合酶链式反应法测定TSHR mRNA及NIS mRNA表达水平。酶联免疫分析法观察血清TPOAb浓度。结果 实验8周，低碘组和高碘组TSHR mRNA及NIS mRNA表达水平均低于适碘组，差异有统计学意义(P<0.05)；且血清TPOAb浓度高于适碘组，但差异无统计学差异(P>0.05)；实验16周，高碘组和低碘组的TSHR mRNA和NISmRNA表达水平均明显低于适碘组，差异有统计学意义(P<0.05)；血清TPOAb浓度高于适碘组，差异有统计学意义(P<0.05)。结论 包括碘过量和碘缺乏在内的长期碘营养障碍均可降低NIS及TSHR的正常表达，同时提高甲状腺自身抗体的浓度，影响甲状腺摄碘功能。
关键词：碘；甲状腺；钠碘转运体；促甲状腺激素受体；甲状腺过氧化物酶抗体
, http://www.100md.com
中图分类号：R581 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.23.023
文章编号：1006-1959(2018)23-0083-03
Abstract：Objective To investigate the effects of different iodine intake on the expression of (TSHR) mRNA， sodium iodide transporter (NIS) mRNA and peroxidase antibody (TPOAb) in rat thyroid stimulating hormone receptor.Methods 30 SD rats were randomly divided into two groups： low iodine group (LI)， normal iodine group (NI) and high iodine group (HI)，) 10 rats in each group. The rats were executed in batches at the 8th ，16th week.The expression levels of TSHR mRNA and NIS mRNA were measured by real-time fluorescence quantitative polymerase chain reaction. The concentration of serum TPOAb was observed by enzyme linked immunosorbent assay.Results At the 8th week of the experiment， the expression of TSHR mRNA and NIS mRNA in iodine deficiency group and high iodine group were lower than that in normal iodine group，the difference was statistically significant (P<0.05).The concentration of serum TPOAb was higher than that of iodine group， but there was no significant difference (P>0.05).At the 16th week of experiment， the expression levels of TSHR mRNA and NISmRNA in high iodine group and low iodine group were significantly lower than those in normal iodine group，the difference was statistically significant (P<0.05).The concentration of serum TPOAb was higher than that of iodine group，the difference was statistically significant (P<0.05).Conclusion Chronic iodine nutritional disorder，including iodine excess and iodine deficiency， can decrease the normal expression of NIS and TSHR， increase the concentration of thyroid autoantibodies and affect the iodine uptake function of thyroid.
, 百拇医药
Key words：Iodine；Thyroid；Sodium iodide transporter；Thyrotropin receptor；Thyroid peroxidase antibody
甲状腺是人体重要的内分泌器官，它通过摄取碘合成甲状腺激素来维持其正常的调节功能 。外界碘环境的变化可通过影响分布于甲状腺滤泡细胞基底膜侧的特异性钠/碘转运体(Na+/I-symporter，NIS)的表达来调节甲状腺组织的摄碘功能[1，2]。与此同时，同样分布于甲状腺滤泡细胞基底膜侧的促甲状腺激素受体(TSHR)可与TSH结合并激活由c-AMP介导的信号级联反应，上调NIS的表達并促进其向细胞膜转运，调节其在滤泡细胞基底膜侧的分布与定位，提高甲状腺组织摄碘率。过氧化物酶(TPO)是甲状腺微粒体抗原的重要组成部分之一 ，与自身免疫性甲状腺疾病的发生发展密切相关。甲状腺过氧化物酶抗体(TPOAb)是甲状腺抑制性抗体，其生成过多会造成甲状腺素的产生减少，影响甲状腺的摄碘功能[3，4]。本实验利用构建不同碘营养水平的大鼠动物模型，动态观察碘对大鼠甲状腺TSHR mRNA及NIS mRNA表达水平的影响，并动态监测长期碘营养障碍对甲状腺自身抗体的影响。, http://www.100md.com(马晔 陈跃宇)
关键词：碘；甲状腺；钠碘转运体；促甲状腺激素受体；甲状腺过氧化物酶抗体
, http://www.100md.com
中图分类号：R581 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.23.023
文章编号：1006-1959(2018)23-0083-03
Abstract：Objective To investigate the effects of different iodine intake on the expression of (TSHR) mRNA， sodium iodide transporter (NIS) mRNA and peroxidase antibody (TPOAb) in rat thyroid stimulating hormone receptor.Methods 30 SD rats were randomly divided into two groups： low iodine group (LI)， normal iodine group (NI) and high iodine group (HI)，) 10 rats in each group. The rats were executed in batches at the 8th ，16th week.The expression levels of TSHR mRNA and NIS mRNA were measured by real-time fluorescence quantitative polymerase chain reaction. The concentration of serum TPOAb was observed by enzyme linked immunosorbent assay.Results At the 8th week of the experiment， the expression of TSHR mRNA and NIS mRNA in iodine deficiency group and high iodine group were lower than that in normal iodine group，the difference was statistically significant (P<0.05).The concentration of serum TPOAb was higher than that of iodine group， but there was no significant difference (P>0.05).At the 16th week of experiment， the expression levels of TSHR mRNA and NISmRNA in high iodine group and low iodine group were significantly lower than those in normal iodine group，the difference was statistically significant (P<0.05).The concentration of serum TPOAb was higher than that of iodine group，the difference was statistically significant (P<0.05).Conclusion Chronic iodine nutritional disorder，including iodine excess and iodine deficiency， can decrease the normal expression of NIS and TSHR， increase the concentration of thyroid autoantibodies and affect the iodine uptake function of thyroid.
, 百拇医药
Key words：Iodine；Thyroid；Sodium iodide transporter；Thyrotropin receptor；Thyroid peroxidase antibody
甲状腺是人体重要的内分泌器官，它通过摄取碘合成甲状腺激素来维持其正常的调节功能 。外界碘环境的变化可通过影响分布于甲状腺滤泡细胞基底膜侧的特异性钠/碘转运体(Na+/I-symporter，NIS)的表达来调节甲状腺组织的摄碘功能[1，2]。与此同时，同样分布于甲状腺滤泡细胞基底膜侧的促甲状腺激素受体(TSHR)可与TSH结合并激活由c-AMP介导的信号级联反应，上调NIS的表達并促进其向细胞膜转运，调节其在滤泡细胞基底膜侧的分布与定位，提高甲状腺组织摄碘率。过氧化物酶(TPO)是甲状腺微粒体抗原的重要组成部分之一 ，与自身免疫性甲状腺疾病的发生发展密切相关。甲状腺过氧化物酶抗体(TPOAb)是甲状腺抑制性抗体，其生成过多会造成甲状腺素的产生减少，影响甲状腺的摄碘功能[3，4]。本实验利用构建不同碘营养水平的大鼠动物模型，动态观察碘对大鼠甲状腺TSHR mRNA及NIS mRNA表达水平的影响，并动态监测长期碘营养障碍对甲状腺自身抗体的影响。, http://www.100md.com(马晔 陈跃宇)