去睾丸老年大鼠血管钙化程度与骨代谢水平研究(1)
摘要：目的 觀察去睾丸老年大鼠血管钙化程度、骨密度(BMD)及骨代谢指标[Ⅰ型前胶原N-端前肽(PⅠNP)、骨钙素(BGP)和Ⅰ型胶原交联羧基末端肽(CTX-Ⅰ)]的变化。方法 取7月龄雄性SD大鼠16只，随机分造模组及对照组。造模组采用双侧睾丸摘除法建立大鼠骨质疏松模型，对照组进行假手术。术后6个月，处死所有大鼠，取右侧股骨以双能X线吸收仪测量BMD，并取胸主动脉用茜素红染色观察钙化情况，用酶联免疫吸附法测量血清PⅠNP、BGP和CTX-Ⅰ浓度。结果 造模组BMD低于对照组[(0.2636±0.0096)g/cm2 vs (0.3104±0.0157)g/cm2]，差异有统计学意义(P<0.05);造模组胸主动脉钙化明显，血清P1NP、BGP含量较对照组降低差异有统计学意义(P<0.05)，而血清CTX-Ⅰ高于对照组，差异有统计学意义(P<0.05)。结论 通过去势法可建立老年大鼠骨质疏松模型，且骨质疏松形成过程中血管钙化程度明显增加，骨形成标志物降低，骨吸收标志物增高。
关键词：去睾丸大鼠;老年性骨质疏松;血管钙化;骨代谢
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中图分类号：R322.7 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.04.002
文章编号：1006-1959(2019)04-0005-03
Abstract：Objective To observevascular calcification， bone mineral density (BMD) and bone metabolism markers in testicular aged rats [type I procollagen N-terminal propeptide (PⅠNP)， osteocalcin (BGP) and type I collagen cross-linked carboxy terminal peptide (CTX) -Ⅰ)] changes. Methods 16 male Sprague-Dawley rats aged 7 months were randomly divided into two groups. In the model group， the rat osteoporosis model was established by bilateral testicular extraction， and the control group was given sham operation. Six months after operation， all rats were sacrificed. BMD was measured by dual-energy X-ray absorptiometry. The thoracic aorta was stained with alizarin red to observe calcification. Serum P1NP and BGP were measured by enzyme-linked immunosorbent assay and CTX-Ⅰ concentration. Results The BMD of the model group was lower than that of the control group [(0.2636±0.0096) g/cm2 vs (0.3104±0.0157) g/cm2]， the difference was statistically significant (P<0.05); The calcification of the thoracic aorta was obvious， and the serum PⅠNP and BGP levels were significantly lower than those of the control group (P<0.05)， while the serum CTX-Ⅰ was higher than the control group， the difference was statistically significant (P<0.05). Conclusion Osteoporosis model can be established in the aged rats by castration method， and the degree of vascular calcification is significantly increased during the process of osteoporosis formation， bone formation markers are decreased， and bone resorption markers are increased.
Key words：Testicular rats;Senile osteoporosis;Vascular calcification;Bone metabolism, http://www.100md.com(杨娜 周进平 林晓容)
关键词：去睾丸大鼠;老年性骨质疏松;血管钙化;骨代谢
, http://www.100md.com
中图分类号：R322.7 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.04.002
文章编号：1006-1959(2019)04-0005-03
Abstract：Objective To observevascular calcification， bone mineral density (BMD) and bone metabolism markers in testicular aged rats [type I procollagen N-terminal propeptide (PⅠNP)， osteocalcin (BGP) and type I collagen cross-linked carboxy terminal peptide (CTX) -Ⅰ)] changes. Methods 16 male Sprague-Dawley rats aged 7 months were randomly divided into two groups. In the model group， the rat osteoporosis model was established by bilateral testicular extraction， and the control group was given sham operation. Six months after operation， all rats were sacrificed. BMD was measured by dual-energy X-ray absorptiometry. The thoracic aorta was stained with alizarin red to observe calcification. Serum P1NP and BGP were measured by enzyme-linked immunosorbent assay and CTX-Ⅰ concentration. Results The BMD of the model group was lower than that of the control group [(0.2636±0.0096) g/cm2 vs (0.3104±0.0157) g/cm2]， the difference was statistically significant (P<0.05); The calcification of the thoracic aorta was obvious， and the serum PⅠNP and BGP levels were significantly lower than those of the control group (P<0.05)， while the serum CTX-Ⅰ was higher than the control group， the difference was statistically significant (P<0.05). Conclusion Osteoporosis model can be established in the aged rats by castration method， and the degree of vascular calcification is significantly increased during the process of osteoporosis formation， bone formation markers are decreased， and bone resorption markers are increased.
Key words：Testicular rats;Senile osteoporosis;Vascular calcification;Bone metabolism, http://www.100md.com(杨娜 周进平 林晓容)