围产期孕妇B族链球菌感染的筛查方法对结果的影响(1)
摘要：目的 通过比较聚合酶链反应(PCR)、显色平板法两种筛查方法，探讨围产期孕妇B族链球菌(GBS)感染的合适快速检测方法。方法 选取2016年5月～2018年5月我院152例常规产检的围产期孕妇，采集孕妇阴道分泌物后分别做PCR检测与显色平板法，并对结果进行质谱检测确认。比较两种方法对GBS的阳性检出率。结果 PCR检测方法的阳性检出率为 6.58%(10/152)，灵敏度为77.27%(17/22)，显色平板法的阳性检出率11.18%(17/152)，灵敏度为50.00%(10/22)。结论 两种筛查方法均能检测GBS，应用显色平板法对筛查围产期孕妇的GBS感染灵敏度更高。临床中对围产期孕妇的GBS 筛查采用显色平板培养的方法能提高GBS的检出率。
关键词：围产期孕妇;B族链球菌;GBS检查;显色平板
中图分类号：R714.7 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.10.057
, 百拇医药
文章编号：1006-1959(2019)10-0171-02
Abstract：Objective A suitable rapid detection method for infection of group B streptococcus (GBS) in perinatal women was conducted by comparing two methods of polymerase chain reaction (PCR) and chromogenic plate method. Methods From May 2016 to May 2018， 152 cases of perinatal pregnant women in our hospital were selected. The vaginal secretions of pregnant women were collected and PCR detection and chromogenic plate method were performed respectively. The results were confirmed by mass spectrometry. The positive detection rate of GBS was compared between the two methods. Results The positive detection rate of PCR detection method was 6.58% (10/152)， the sensitivity was 77.27% (17/22)， the positive detection rate of chromogenic plate method was 11.18% (17/152)， and the sensitivity was 50.00%(10/22). Conclusion Both screening methods can detect GBS， and the chromogenic plate method is more sensitive to screening GBS infection in perinatal women. In clinical practice， GBS screening for perinatal pregnant women can improve the detection rate of GBS by using chromogenic plate culture.
, http://www.100md.com
Key words：Perinatal pregnant women;Group B streptococcus;GBS examination;Chromogenic plate
B族鏈球菌(group B streptococcus，GBS)亦称无乳链球菌，为革兰阳性菌，属于条件致病菌，是一种可定植于孕产妇肠道和阴道的兼性厌氧菌。孕妇GBS感染可引起胎膜早破、流产以及绒毛膜羊膜炎等，也是新生儿早发型GBS感染的主要危险因素，据文献报道，有40%～70%的携带GBS围产期孕妇在生产过程感染新生儿，引起新生儿败血症、肺炎等严重疾病[1]。GBS首次被发现会导致围产期孕妇死亡是在1938年，正常情况下非妊娠女性的阴道和肛门也有GBS定植的现象，但是致病风险很小，然而对于围产期的孕妇来说，由于体内激素水平、特殊的生理生化指标的改变，GBS的致病率远远高于非妊娠女性，此外，GBS也会以垂直传播的方式使新生儿感染。由此可见，GBS的危害不容小觑[2]。本研究对我院152例常规产检的围产期孕妇采集孕妇阴道分泌物后分别做PCR检测与显色平板法，以期为围产期孕妇人群做好GBS感染的筛查提供依据。
1资料与方法
1.1一般资料 选取2016年5月～2018年5月来天津市和平区妇产科医院进行常规产检的152例围产期孕妇。纳入对本次研究内容全部知晓且自愿参与者，无流产病例，排除有夫妻生活者以及使用抗生素者。本次研究经过医院伦理委员会同意。孕妇孕周35～37周，平均孕周(36.23±0.68)周，年龄22～41岁，平均年龄(26.22±4.23)岁。首次妊娠者122例，有分娩史者30例，所有孕妇均接受GBS感染筛查。, http://www.100md.com(呼蓓蓓)
关键词：围产期孕妇;B族链球菌;GBS检查;显色平板
中图分类号：R714.7 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.10.057
, 百拇医药
文章编号：1006-1959(2019)10-0171-02
Abstract：Objective A suitable rapid detection method for infection of group B streptococcus (GBS) in perinatal women was conducted by comparing two methods of polymerase chain reaction (PCR) and chromogenic plate method. Methods From May 2016 to May 2018， 152 cases of perinatal pregnant women in our hospital were selected. The vaginal secretions of pregnant women were collected and PCR detection and chromogenic plate method were performed respectively. The results were confirmed by mass spectrometry. The positive detection rate of GBS was compared between the two methods. Results The positive detection rate of PCR detection method was 6.58% (10/152)， the sensitivity was 77.27% (17/22)， the positive detection rate of chromogenic plate method was 11.18% (17/152)， and the sensitivity was 50.00%(10/22). Conclusion Both screening methods can detect GBS， and the chromogenic plate method is more sensitive to screening GBS infection in perinatal women. In clinical practice， GBS screening for perinatal pregnant women can improve the detection rate of GBS by using chromogenic plate culture.
, http://www.100md.com
Key words：Perinatal pregnant women;Group B streptococcus;GBS examination;Chromogenic plate
B族鏈球菌(group B streptococcus，GBS)亦称无乳链球菌，为革兰阳性菌，属于条件致病菌，是一种可定植于孕产妇肠道和阴道的兼性厌氧菌。孕妇GBS感染可引起胎膜早破、流产以及绒毛膜羊膜炎等，也是新生儿早发型GBS感染的主要危险因素，据文献报道，有40%～70%的携带GBS围产期孕妇在生产过程感染新生儿，引起新生儿败血症、肺炎等严重疾病[1]。GBS首次被发现会导致围产期孕妇死亡是在1938年，正常情况下非妊娠女性的阴道和肛门也有GBS定植的现象，但是致病风险很小，然而对于围产期的孕妇来说，由于体内激素水平、特殊的生理生化指标的改变，GBS的致病率远远高于非妊娠女性，此外，GBS也会以垂直传播的方式使新生儿感染。由此可见，GBS的危害不容小觑[2]。本研究对我院152例常规产检的围产期孕妇采集孕妇阴道分泌物后分别做PCR检测与显色平板法，以期为围产期孕妇人群做好GBS感染的筛查提供依据。
1资料与方法
1.1一般资料 选取2016年5月～2018年5月来天津市和平区妇产科医院进行常规产检的152例围产期孕妇。纳入对本次研究内容全部知晓且自愿参与者，无流产病例，排除有夫妻生活者以及使用抗生素者。本次研究经过医院伦理委员会同意。孕妇孕周35～37周，平均孕周(36.23±0.68)周，年龄22～41岁，平均年龄(26.22±4.23)岁。首次妊娠者122例，有分娩史者30例，所有孕妇均接受GBS感染筛查。, http://www.100md.com(呼蓓蓓)