多重实时荧光PCR在诊断学龄前期CAP中应用价值樊旭成 薛娜 韩志国 等(160)
摘要：目的 探讨多重实时荧光聚合酶链式反应(PCR)在学龄前期社区获得性肺炎(CAP)诊断中的应用价值。方法 筛选2018年1月～12月到乌鲁木齐市某社区服务门诊就诊的206例CAP患儿，给予常规采血检测降钙素原(PCT)和C-反应蛋白(CRP)水平，同时所有患儿均接受多重实时荧光PCR检测感染病原体并比较。结果 常规采血方法检测结果呈阳性有116例患儿，占56.31%;采用多重实时荧光PCR检测结果呈阳性共有201例患儿，占97.57%。采用多重实时荧光PCR检测的结果阳性率高于常规采血检测，差异具有统计学意义(P<0.05)。结论 多重实时荧光PCR在学龄前期社区获得性感染中的应用效果良好，具有很高的灵敏度，方便快捷，能够提供较高价值的诊疗参考。
关键词：多重实时荧光PCR;学龄前期;社区获得性感染
中圖分类号：R446 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.13.051
文章编号：1006-1959(2019)13-0160-02
Abstract：Objective To investigate the value of multiple real-time fluorescent polymerase chain reaction (PCR) in the diagnosis of pre-school community-acquired pneumonia(CAP). Methods A total of 206 children with CAP who were admitted to a community outpatient clinic in Urumqi from January to December 2018 were enrolled. The routine blood sampling was performed to detect procalcitonin (PCT) and C-reactive protein (CRP) levels. Multiple real-time fluorescent PCR was performed to detect infectious pathogens and compare them. Results The results of routine blood collection were positive in 116 cases， accounting for 56.31%. The results of multiple real-time fluorescent PCR were positive in 201 cases， accounting for 97.57%. The positive rate of multiple real-time fluorescent PCR was higher than that of conventional blood sampling，the difference was statistically significant (P<0.05). Conclusion Multiple real-time fluorescent PCR has a good effect in pre-school community acquired infections. It has high sensitivity， is convenient and fast， and can provide high-value diagnosis and treatment reference.
Key words：Multiplex real-time fluorescent PCR;Preschool;Community acquired infection
社区获得性肺炎(community-acquired pneumonia，CAP)是指在医院外患上感染性肺炎，包括感染了病原体在入院后潜伏期内发病的肺炎，属于一种感染性疾病，严重威胁人类健康，也是造成儿童死亡的重要疾病之一。世界卫生组织等机构的报道，2016年5岁以下儿童的死亡人数有560万，其中肺炎是造成死亡的重要传染病[1]。据文献研究显示，中国每年有大约2000万儿童肺炎患者，其中大约35万5岁以下患儿死于肺炎，肺炎是造成我国婴幼儿死亡的第1位疾病[2]。因此，减少肺炎的发生，及早诊断感染病因并给与相应的治疗，提高治愈率，是一项急迫的任务。目前常规检测方法是采血检测，但是此类方法存在一定的局限性，明确CAP病原比较困难。有研究显示，与传统的检测方法相比，多重实时荧光聚合酶链式反应(polymerase chain reaction，PCR)技术具有取样少、灵敏度高、简单快捷等优点[3]。本文旨在探讨多重实时荧光PCR在诊断学龄前期社区获得性感染中的应用价值，现报告如下。
1资料与方法
1.1一般资料 筛选2018年1月～12月到乌鲁木齐市某社区服务门诊就诊的CAP患儿。纳入标准：①依据最新《儿童社区获得性肺炎管理指南》确诊的CAP患儿;②年龄28 d～7岁。排除标准：①先天性免疫缺陷的患儿;②肺结核病患儿;③合并其他基础疾病患儿。最终入选206例患儿作为研究对象，其中男105例，女101例，年龄28 d～7岁，中位年龄3.26岁。患儿家属知情且签署自愿书。, 百拇医药(樊旭成 薛娜 韩志国 高枫)
关键词：多重实时荧光PCR;学龄前期;社区获得性感染
中圖分类号：R446 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.13.051
文章编号：1006-1959(2019)13-0160-02
Abstract：Objective To investigate the value of multiple real-time fluorescent polymerase chain reaction (PCR) in the diagnosis of pre-school community-acquired pneumonia(CAP). Methods A total of 206 children with CAP who were admitted to a community outpatient clinic in Urumqi from January to December 2018 were enrolled. The routine blood sampling was performed to detect procalcitonin (PCT) and C-reactive protein (CRP) levels. Multiple real-time fluorescent PCR was performed to detect infectious pathogens and compare them. Results The results of routine blood collection were positive in 116 cases， accounting for 56.31%. The results of multiple real-time fluorescent PCR were positive in 201 cases， accounting for 97.57%. The positive rate of multiple real-time fluorescent PCR was higher than that of conventional blood sampling，the difference was statistically significant (P<0.05). Conclusion Multiple real-time fluorescent PCR has a good effect in pre-school community acquired infections. It has high sensitivity， is convenient and fast， and can provide high-value diagnosis and treatment reference.
Key words：Multiplex real-time fluorescent PCR;Preschool;Community acquired infection
社区获得性肺炎(community-acquired pneumonia，CAP)是指在医院外患上感染性肺炎，包括感染了病原体在入院后潜伏期内发病的肺炎，属于一种感染性疾病，严重威胁人类健康，也是造成儿童死亡的重要疾病之一。世界卫生组织等机构的报道，2016年5岁以下儿童的死亡人数有560万，其中肺炎是造成死亡的重要传染病[1]。据文献研究显示，中国每年有大约2000万儿童肺炎患者，其中大约35万5岁以下患儿死于肺炎，肺炎是造成我国婴幼儿死亡的第1位疾病[2]。因此，减少肺炎的发生，及早诊断感染病因并给与相应的治疗，提高治愈率，是一项急迫的任务。目前常规检测方法是采血检测，但是此类方法存在一定的局限性，明确CAP病原比较困难。有研究显示，与传统的检测方法相比，多重实时荧光聚合酶链式反应(polymerase chain reaction，PCR)技术具有取样少、灵敏度高、简单快捷等优点[3]。本文旨在探讨多重实时荧光PCR在诊断学龄前期社区获得性感染中的应用价值，现报告如下。
1资料与方法
1.1一般资料 筛选2018年1月～12月到乌鲁木齐市某社区服务门诊就诊的CAP患儿。纳入标准：①依据最新《儿童社区获得性肺炎管理指南》确诊的CAP患儿;②年龄28 d～7岁。排除标准：①先天性免疫缺陷的患儿;②肺结核病患儿;③合并其他基础疾病患儿。最终入选206例患儿作为研究对象，其中男105例，女101例，年龄28 d～7岁，中位年龄3.26岁。患儿家属知情且签署自愿书。, 百拇医药(樊旭成 薛娜 韩志国 高枫)