DLPME-HPLC法同时测定人血清中维生素A和维生素E含量(1)
摘要：目的 建立分散液相微萃取(DLPME)-高效液相色谱(HPLC)同时测定人血清中维生素A、E含量的方法。方法 2018年5月随机抽取人血液样本50例，经DLPME技术处理后，采用HPLC对人血清中维生素A、E含量进行检测，使用标准曲线法进行定量分析。结果 维生素A标准曲线方程为y=13225x+410.03，相关系数为0.9998，检出限为0.042 μg/ml;维生素E标准曲线方程为y=1383.9x-458.27，相关系数为0.9997，检出限为0.096 μg/ml。维生素A和维生素E的加标回收率分别为90.12%～103.70%和81.09%～92.21%，含量范围分别为(0.62±0.04)μg/ml和(8.41±0.43)μg/ml。结论 DLPME-HPLC法操作简便，节约试剂，对样本富集效率高，通过该方法的研究，对微量血清中维生素A和维生素E的测定具很好的的应用价值。
关键词：分散液相微萃取;高效液相色谱;人血清;维生素A;维生素E
中圖分类号：R151.2 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.20.051
文章编号：1006-1959(2019)20-0161-03
Simultaneous Determination of Vitamin A and Vitamin E in Human Serum by DLPME-HPLC
ZHANG Xiao-min，WANG Jie，WANG Shu-jing，ZHU Jia-jia
Abstract：Objective To establish a method for simultaneous determination of vitamin A and E in human serum by dispersive liquid phase microextraction (DLPME)-high performance liquid chromatography (HPLC). Methods In May 2018， 50 human blood samples were randomly selected. After treatment with DLPME technique， the contents of vitamin A and E in human serum were detected by HPLC and quantified by standard curve method.Results The standard curve of vitamin A was y=13225x+410.03， the correlation coefficient was 0.9998， the detection limit was 0.042 μg/ml; the standard curve of vitamin E was y=1383.9x-458.27， the correlation coefficient was 0.9997， and the detection limit was 0.096 μg/ml. The recoveries of vitamin A and vitamin E were 90.12%～103.70% and 81.09%～92.21%， respectively， and the contents ranged from (0.62±0.04)μg/ml and (8.41±0.43)μg/ml.Conclusion DLPME-HPLC method is easy to operate， saves reagents， and has high efficiency of sample enrichment. The research of this method has a good application value for the determination of vitamin A and vitamin E in trace serum.
Key words：Dispersive liquid phase microextraction;High performance liquid chromatography;Human serum;Vitamin A;Vitamin E
维生素(vitamin)是生物体所需要的微量营养成分，维生素A和维生素E是维持人体正常代谢功能所必须的维生素，在自身体内无法自行合成，需要通过饮食等手段获得[1]。维生素A和维生素E均属于脂溶性的醇类物质，维生素A可维持上皮细胞的正常代谢，促进免疫球蛋白的合成;维生素E能够抑制脂肪酸的氧化，减少脂褐质的形成，并保护细胞免受自由基的损害，具有抗衰老、保护皮肤等作用[2]。日常生活中，人体对维生素的需要量较小，常以毫克或微克计算，但一旦缺乏就会引发相应的维生素缺乏症，对人体健康造成损害。因此，测定人血清中维生素A和维生素E对于维生素缺乏诊断具有重要意义。分散液相微萃取-高效液相色谱(DLPME-HPLC)是一新型前处理技术，该方法及采样、萃取和浓缩为一体，具有操作简单、快速、效率低等特点。本文主要建立DLPME-HPLC同时测定人血清中维生素A、E含量的方法，现报道如下。, http://www.100md.com(张小敏 王洁 王淑静 朱佳佳)
关键词：分散液相微萃取;高效液相色谱;人血清;维生素A;维生素E
中圖分类号：R151.2 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.20.051
文章编号：1006-1959(2019)20-0161-03
Simultaneous Determination of Vitamin A and Vitamin E in Human Serum by DLPME-HPLC
ZHANG Xiao-min，WANG Jie，WANG Shu-jing，ZHU Jia-jia
Abstract：Objective To establish a method for simultaneous determination of vitamin A and E in human serum by dispersive liquid phase microextraction (DLPME)-high performance liquid chromatography (HPLC). Methods In May 2018， 50 human blood samples were randomly selected. After treatment with DLPME technique， the contents of vitamin A and E in human serum were detected by HPLC and quantified by standard curve method.Results The standard curve of vitamin A was y=13225x+410.03， the correlation coefficient was 0.9998， the detection limit was 0.042 μg/ml; the standard curve of vitamin E was y=1383.9x-458.27， the correlation coefficient was 0.9997， and the detection limit was 0.096 μg/ml. The recoveries of vitamin A and vitamin E were 90.12%～103.70% and 81.09%～92.21%， respectively， and the contents ranged from (0.62±0.04)μg/ml and (8.41±0.43)μg/ml.Conclusion DLPME-HPLC method is easy to operate， saves reagents， and has high efficiency of sample enrichment. The research of this method has a good application value for the determination of vitamin A and vitamin E in trace serum.
Key words：Dispersive liquid phase microextraction;High performance liquid chromatography;Human serum;Vitamin A;Vitamin E
维生素(vitamin)是生物体所需要的微量营养成分，维生素A和维生素E是维持人体正常代谢功能所必须的维生素，在自身体内无法自行合成，需要通过饮食等手段获得[1]。维生素A和维生素E均属于脂溶性的醇类物质，维生素A可维持上皮细胞的正常代谢，促进免疫球蛋白的合成;维生素E能够抑制脂肪酸的氧化，减少脂褐质的形成，并保护细胞免受自由基的损害，具有抗衰老、保护皮肤等作用[2]。日常生活中，人体对维生素的需要量较小，常以毫克或微克计算，但一旦缺乏就会引发相应的维生素缺乏症，对人体健康造成损害。因此，测定人血清中维生素A和维生素E对于维生素缺乏诊断具有重要意义。分散液相微萃取-高效液相色谱(DLPME-HPLC)是一新型前处理技术，该方法及采样、萃取和浓缩为一体，具有操作简单、快速、效率低等特点。本文主要建立DLPME-HPLC同时测定人血清中维生素A、E含量的方法，现报道如下。, http://www.100md.com(张小敏 王洁 王淑静 朱佳佳)