IL-10基因-819T/C多态性与糖尿病肾病易感性的Meta分析(1)
摘要：目的 评价白细胞介素10(IL-10)基因-819T/C多态位点与糖尿病肾病(DN)易感性的关系。方法 检索PubMed、Embase、Medline、中国知网、维普数据库、万方数据库中与IL-10基因-819T/C多态性及DN发病相关的研究，检索时间为建库至2019年10月，使用纽卡斯尔渥太华(NOS)标准对纳入文献进行质量评价。以发病风险比值比(OR)及95%可信区间(CI)为效应指标，使用STATA12.0进行统计学分析。结果 共纳入7项病例对照研究，其中实验组均为DN患者，对照组为糖尿病(DM)患者或健康对照者(HC)。Meta分析結果显示：①DN组与对照组相比，IL-10基因-819T/C位点在等位基因模型(C vs. T：OR=0.999，P=0.990)、纯合子模型(CC vs. TT：OR=1.040，P=0.713)、显性模型(TC+CC vs. TT：OR=1.052，P=0.561)、隐性模型(CC vs. TC+TT：OR=0.993，P=0.954)、杂合子模型(TC vs. TT：OR=1.039，P=0.679)上的差异均无统计学意义(P>0.05);②地区亚组分析显示，在亚洲及中国人群中，DN组与对照组在各遗传模型上的差异均无统计学意义(P>0.05)。结论 IL-10基因-819T/C多态性不增加DN易感性，在亚洲及中国地区人群中可得到类似结论，但仍需高质量的研究进一步验证。
关键词：IL-10基因;-819T/C多态性;糖尿病肾病;易感性
Abstract：Objective To evaluate the relationship between interleukin 10 (IL-10) gene-819T/C polymorphism and susceptibility to diabetic nephropathy (DN).Methods To search the studies related to IL-10 gene-819T/C polymorphism and DN incidence in PubMed， Embase， Medline， China HowNet， Weipu database， Wanfang database. The search time was from the establishment of the database to October 2019， use The Newcastle Ottawa (NOS) standard evaluates the quality of the included literature. Taking morbidity risk ratio (OR) and 95% confidence interval (CI) as the effect indicators， STATA12.0 was used for statistical analysis.Results A total of 7 case-control studies were included， in which the experimental group was all patients with DN， and the control group was patients with diabetes (DM) or healthy controls (HC). Meta analysis results showed that： ①Compared with the control group in the DN group，IL-10 gene-819T / C locus is in allele model (C vs. T： OR=0.999， P=0.990)， homozygous model (CC vs. TT： OR=1.040， P=0.713)， dominant Model (TC+CC vs. TT：OR=1.052， P=0.561)， recessive model (CC vs. TC+TT：OR=0.993， P=0.954)， heterozygous model (TC vs. TT：OR=1.039，P=0.679)， there was no statistically significant difference(P>0.05); ②Analysis of regional subgroups showed that in Asian and Chinese populations， there were no statistically significant differences in genetic models between the DN group and the control group(P>0.05). Conclusion The IL-10 gene-819T/C polymorphism does not increase the susceptibility to DN. Similar conclusions can be obtained in populations in Asia and China， but high-quality research is still needed to further verify.
Key words：IL-10 gene;-819T / C polymorphism;Diabetic nephropathy;Susceptibility
糖尿病肾病(DN)是糖尿病(DM)常见的微血管并发症之一，也是患者死亡的重要原因，其主要表现为尿白蛋白排泄率增加和肾功能减退[1]。研究显示[2]，炎性细胞因子是DN发病的重要因素之一。白细胞介素10(IL-10)是由辅助性T细胞产生的一种有效的免疫调节细胞因子，具有抗炎和免疫抑制双重作用[3]。Mysliwska J等[4]研究发现，DN患者血清IL-10浓度升高与肾脏损伤程度密切相关。同时，咸伟等[5]发现，DN患者血清IL-10的水平随DN分级的增加呈上升趋势。IL-10基因位于染色体1q31-32区域，由4个内含子及5个外显子组成[6]，IL-10基因启动子区域存在多个核苷酸多态位点，IL-10-819T/C位点突变可能导致启动子序列的改变，调控下游mRNA含量，导致IL-10表达异常[6]。目前有部分关于IL-10基因-819T/C多态性及DN易感性的研究，但其结论仍是存在争议的[8-14]。因此本研究运用Meta分析方法，旨在对IL-10-819T/C多态性与DN易感性间的关系进行综合评估，以获得更加可靠的结论。, 百拇医药(胡玲 王思思 周乐汀)
关键词：IL-10基因;-819T/C多态性;糖尿病肾病;易感性
Abstract：Objective To evaluate the relationship between interleukin 10 (IL-10) gene-819T/C polymorphism and susceptibility to diabetic nephropathy (DN).Methods To search the studies related to IL-10 gene-819T/C polymorphism and DN incidence in PubMed， Embase， Medline， China HowNet， Weipu database， Wanfang database. The search time was from the establishment of the database to October 2019， use The Newcastle Ottawa (NOS) standard evaluates the quality of the included literature. Taking morbidity risk ratio (OR) and 95% confidence interval (CI) as the effect indicators， STATA12.0 was used for statistical analysis.Results A total of 7 case-control studies were included， in which the experimental group was all patients with DN， and the control group was patients with diabetes (DM) or healthy controls (HC). Meta analysis results showed that： ①Compared with the control group in the DN group，IL-10 gene-819T / C locus is in allele model (C vs. T： OR=0.999， P=0.990)， homozygous model (CC vs. TT： OR=1.040， P=0.713)， dominant Model (TC+CC vs. TT：OR=1.052， P=0.561)， recessive model (CC vs. TC+TT：OR=0.993， P=0.954)， heterozygous model (TC vs. TT：OR=1.039，P=0.679)， there was no statistically significant difference(P>0.05); ②Analysis of regional subgroups showed that in Asian and Chinese populations， there were no statistically significant differences in genetic models between the DN group and the control group(P>0.05). Conclusion The IL-10 gene-819T/C polymorphism does not increase the susceptibility to DN. Similar conclusions can be obtained in populations in Asia and China， but high-quality research is still needed to further verify.
Key words：IL-10 gene;-819T / C polymorphism;Diabetic nephropathy;Susceptibility
糖尿病肾病(DN)是糖尿病(DM)常见的微血管并发症之一，也是患者死亡的重要原因，其主要表现为尿白蛋白排泄率增加和肾功能减退[1]。研究显示[2]，炎性细胞因子是DN发病的重要因素之一。白细胞介素10(IL-10)是由辅助性T细胞产生的一种有效的免疫调节细胞因子，具有抗炎和免疫抑制双重作用[3]。Mysliwska J等[4]研究发现，DN患者血清IL-10浓度升高与肾脏损伤程度密切相关。同时，咸伟等[5]发现，DN患者血清IL-10的水平随DN分级的增加呈上升趋势。IL-10基因位于染色体1q31-32区域，由4个内含子及5个外显子组成[6]，IL-10基因启动子区域存在多个核苷酸多态位点，IL-10-819T/C位点突变可能导致启动子序列的改变，调控下游mRNA含量，导致IL-10表达异常[6]。目前有部分关于IL-10基因-819T/C多态性及DN易感性的研究，但其结论仍是存在争议的[8-14]。因此本研究运用Meta分析方法，旨在对IL-10-819T/C多态性与DN易感性间的关系进行综合评估，以获得更加可靠的结论。, 百拇医药(胡玲 王思思 周乐汀)
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