玉米苞叶中芦丁的含量测定(1)
【摘 要】 目的： 建立HPLC法测定玉米苞叶中芦丁含量的方法。方法：采用高效液相色谱法，以甲醇为提取溶剂，超声辅助提取玉米苞叶中的芦丁，色谱柱为phenomenon C18 (5μm，250mm×4.6mm);检测波长为280nm;流动相采用乙腈-0.1%冰醋酸(20∶80);流速为0.8mL/min;柱温为25℃;进样量10μL。结果：芦丁进样量在0.19470～2.0047μg·mL-1范围内与峰面积呈良好的线性关系(r=0.9996)，平均加样回收率为97.70%，RSD为1.46%(n=6)。结论：该测定方法简便、快捷，重现性好，可为玉米苞叶药材质量控制和评价提供参考。
【关键词】 玉米苞叶;芦丁;含量测定;高效液相色谱法
【中图分类号】R284.2 【文献标志码】 A【文章编号】1007-8517(2019)22-0028-04
Determination of Rutin in Corn Bracts
ZHOU Shuguang1 ZHEN Dandan2* MO Yanheng3 HE Liuyan3 ZHEN Hanshen2
1.The Guangxi Zhuang Autonomous Region people’s Hospital，Nanning 530021，China;
2. Guangxi University of Traditional Chinese Medicine，Nanning 530001，China;
3.Guangxi Medical College，Guilin 541004，China
Abstract：Objective To establish a HPLC method for determination of rutin in corn husks. Methods High performance liquid chromatography (HPLC) was used to extract rutin from corn husks with methanol as the extraction solvent. The chromatographic column was phenomenon C18 (5μm，250mm×4.6mm)，the detection wavelength is 280nm;the mobile phase was acetonitrile-0.1% glacial acetic acid (20∶80);the flow rate was 0.8mL/minccolumn temperature is 25 ℃;sample quantity is 10μL.Results There was a good linear relationship between the amount of rutin injected and the peak area within the range of 0.19470～2.0047μg·mL-1 (r=0.9996)， and the average recovery rate was 97.70%. RSD was 1.46% (n=6).Conclusion the method is simple， rapid and reproducible， which can provide reference for quality control and evaluation of bract.
Keywords：Cornhusks;Rutin;Content;Determination;Highperformance Liquid Chromatography
玉米苞葉(corn husks)，是禾本科玉蜀黍属植物玉米(Zea mays L)[1]果穗外部的包被部分。玉米苞叶含有大量的纤维素、一定量的淀粉、总黄酮及单糖多糖等活性成分。2017年我国玉米产量为21 589万吨，其中玉米副产物产量达到百万吨级，大多数的玉米苞叶被, 百拇医药(周曙光 甄丹丹 莫缓恒 何柳艳 甄汉深)
【关键词】 玉米苞叶;芦丁;含量测定;高效液相色谱法
【中图分类号】R284.2 【文献标志码】 A【文章编号】1007-8517(2019)22-0028-04
Determination of Rutin in Corn Bracts
ZHOU Shuguang1 ZHEN Dandan2* MO Yanheng3 HE Liuyan3 ZHEN Hanshen2
1.The Guangxi Zhuang Autonomous Region people’s Hospital，Nanning 530021，China;
2. Guangxi University of Traditional Chinese Medicine，Nanning 530001，China;
3.Guangxi Medical College，Guilin 541004，China
Abstract：Objective To establish a HPLC method for determination of rutin in corn husks. Methods High performance liquid chromatography (HPLC) was used to extract rutin from corn husks with methanol as the extraction solvent. The chromatographic column was phenomenon C18 (5μm，250mm×4.6mm)，the detection wavelength is 280nm;the mobile phase was acetonitrile-0.1% glacial acetic acid (20∶80);the flow rate was 0.8mL/minccolumn temperature is 25 ℃;sample quantity is 10μL.Results There was a good linear relationship between the amount of rutin injected and the peak area within the range of 0.19470～2.0047μg·mL-1 (r=0.9996)， and the average recovery rate was 97.70%. RSD was 1.46% (n=6).Conclusion the method is simple， rapid and reproducible， which can provide reference for quality control and evaluation of bract.
Keywords：Cornhusks;Rutin;Content;Determination;Highperformance Liquid Chromatography
玉米苞葉(corn husks)，是禾本科玉蜀黍属植物玉米(Zea mays L)[1]果穗外部的包被部分。玉米苞叶含有大量的纤维素、一定量的淀粉、总黄酮及单糖多糖等活性成分。2017年我国玉米产量为21 589万吨，其中玉米副产物产量达到百万吨级，大多数的玉米苞叶被, 百拇医药(周曙光 甄丹丹 莫缓恒 何柳艳 甄汉深)