[摘要]目的:观察不同浓度外源性雌激素己烯雌酚(Diaethylstilbestro，DES)对体外培养人正常黑素细胞的细胞增殖及酪氨酸酶活性的影响。方法:采用MTT法测定黑素细胞增殖情况，参考Nakajima M方法测定酪氨酸酶活性。结果:不同浓度己烯雌酚对体外培养人黑素细胞增殖及酪氨酸酶活性均有促进作用。结论:己烯雌酚能促进人黑素细胞增殖及酪氨酸酶活性，对相关皮肤色素性疾病可能有一定的治疗作用。

    [关键词]己烯雌酚；黑素细胞；酪氨酸酶

    [中图分类号]R329.25[文献标识码]A[文章编号]1008-6455(2008)05-03

    Effects of Diaethylstilbestro(DES) on cultured human melanocytes in vitro

    GONG Shi1, ZHANG Qing2, ZHAO Zhi-Guo2,lIU Qiao 1,CHENG Yong-jun1,WANG Ai-min1,YANGXian-xiu1 ,CHEN Kang1

    (1.Department of Dermatology,Hai nan Provincial Skin Disease Hospital,Haikou, 570206 Hainan,China; 2.Department of Pharmacology, Guiyang Medical College) ......