黄褐斑病损的组织学特征及角蛋白10的表达(1)
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[摘要]目的:研究黄褐斑皮肤的组织学改变及角蛋白10(CK10) 的表达情况。方法:取黄褐斑患者睑袋术后外眦多余皮肤,行石蜡切片的HE染色、Fontana-Masson染色和CK10的免疫组化染色,观察此处皮肤的病理学改变和CK10的分布情况。结果:黄褐斑皮损处表皮变薄,基底层细胞周围可见明显色素颗粒,基底上层、毛囊上段、真皮层也有色素颗粒分布,真皮胶原紊乱、断裂,皮肤附属器数量较少。CK10主要分布在表皮的基底上层和皮脂腺细胞的胞浆和腺泡的外层。结论:黄褐斑病损皮肤各层黑色素含量增多,皮肤组织结构多有老化表现。CK10在基底上层表达增强,其表达增强可能与表皮细胞过度分化、皮肤老化有关。
[关键词]黄褐斑;色素性疾病;组织结构;角蛋白10;皮肤老化
[中图分类号]R758.4+2[文献标识码]A[文章编号]1008-6455(2010)09-1340-03
Histological features of melasma lesions and the expression of keratin 10
TANG Jian-bing1,CHENG Biao1,Zhang Wei2, LI Qin1,LIU Da-Lie1, YU Wen-Lin1,ZENG Dong1,LIAO Yuan-xing1
(1.Plastic and Aesthetic Center;2.Department of Pathology, Guang zhou Military Command General Hospital, Guangzhou 510010 Guang dong,China)
Abstract: Objective To investigate the histological changes of the skin with melasma and keratin 10 (CK10) expression. Methods The excess skin in the lateral canthus was acquired after eyelid plasty operation for melasma patients,the paraffin sections was stained with HE,Fontana-Masson and CK10 immunohistochemical staining to observe the pathological changes of the melasma skin and the distribution of CK10.ResultsThe epidermis was thin in the melasma skin. And the melanin can be seen clearly around the basal layer cells.It could be seen in the upper basal layer, the upper hair follicle and dermis. Dermal collagen was disorder, fracture, the number of skin appendages was less than the normal skin. CK10 mainly distributed in the upper stratum of basal epidermis and the most outer layer of sebaceous glands. ConclusionThe content of melanin increases in melasma skin, and skin aging features appear in the histological structure. CK10 expresses strongly in the upper stratum of basal epidermis which maybe relate with the excessive differentiation of epidermal cells and the skin aging.
Key words: melasma; pigment disorders; histological structure; keratin 10; skin aging
黄褐斑是临床常见的一种获得性色素增加性皮肤病,亚洲人种以面部的颧颊、下睑、鼻根等部位好发,关于黄褐斑组织病理改变方面的研究还比较少。角蛋白属于细胞骨架中中间丝蛋白家族,它是在细胞的增殖、分化和成熟过程中形成的,细胞角蛋白的表达情况也间接反映了细胞的增殖和分化情况[1]。我们利用常规HE染色、Fontana-Masson染色和免疫组化染色观察了黄褐斑皮损的组织结构变化和角蛋白10的分布情况。
1 材料和方法
1.1 标本:取16例黄褐斑患者的睑袋整形术后外眦处多余皮肤组织做病理检测,取5例无明显色斑的睑袋患者术后皮肤作对照检测。所有黄褐斑患者均符合中国中西医结合学会皮肤性病专业委员会色素病学组《关于黄褐斑的临床诊断和疗效标准》(2003年修订稿)[2] ......
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