不同配比纳米羟基磷灰石/聚己内酯复合材料细胞相容性的研究(1)
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[摘要]目的：观察不同配比nPCL/HA电纺纤维取向薄膜材料的细胞相容性。方法：将人骨髓间充质干细胞(hBMSCs)体外诱导培养为成骨细胞；并经传代培养第5代的人骨髓间充质干细胞，以2×105cm2的密度与不同配比nPCL/HA电纺纤维取向薄膜支架在培养板内共培养，同时以nPCL电纺纤维非取向薄膜材料作为对照，初步观察hBMSCs在不同配比nPCL/HA支架材料上复合培养，对其细胞相容性进行评价。结果：hBMSCs与3种电纺薄膜支架材料均有细胞相容性，细胞能在不同材料表面黏附生长、分化增殖。但是PCL/ HA的配比为20:1电纺纤维取向薄膜材料黏附率(35.3±2.6)%，为3中材料中黏附率最高的一种，材料表面细胞生长良好，体积变大，有伪足生长。结论：PCL/ HA的配比为20：1电纺纤维取向薄膜材料，较适合作为支架材料应用于hBMSCs为种子细胞的组织工程构建。
[关键词]骨髓间充质干细胞；聚己内酯；羟基磷灰石；细胞相容性
[中图分类号]Q813.1[文献标识码]A [文章编号]1008-6455(2011)02-0243-04
Cellular biocompatibility of various electrospunnPCL/HA scaffold materials
LI Jia-feng1,WAN Mei-rong2,GUAN Hai-hong1,HE Wen-peng1,ZHANG Hong-chuang1,ZHANG Yang1,CHEN Li-juan1
(1.Department of Oral Maxillofacial Surgery, Affiliated Xuzhou City Hospital of Xuzhou Medical College; 2.Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu,China)
Abstract:ObjectiveTo investigate cellular biocompatibilityof different nPCL/HA scaffold materials.MethodsElectrostatic spinning or electrospinning is an interesting method for producing nonwoven fibers with diameters of submicrometers down to nanometers. Nanofibrous membranes were used in many biomedical applications including drug delivery, wound healing and scaffolding for tissue engineering. Novel bone-scaffolding materials were successfully fabricated by electrospinning from polycaprolactone(PCL) solutions containing nanoparticles of hydroxyapatite(HA). In intro cultured hBMSCs(5th generation) were seeded at the density of 2×105 cell/cm2 onto scaffolds ofnPCL/HA and nPCL as control. The cell-material complex was observed in order to evaluate the cellular biocompatibilitybetween cells and materials.ResultsHBMSCs were shown good adhesion to all 3 types of scaffolds after seeding. The cellular biocompatibilityof nPCL/HA(20:1) (35.3±2.6)% washigher than the others.ConclusionNano-PCL/HA(20:1) was shown significantly higher adhesion rate to hBMSCs, and could be used as scaffold material in the field of bone tissue engineering.
Key words:bone marrow stromal cells; polycaprolactone; hydroxypatite; cellular biocompatibility ......
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