咖啡因增强UVB辐射诱导的HaCaT细胞凋亡的研究(1)
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[摘要]目的：观察咖啡因对中波紫外线(Ultraviolet B ，UVB)辐射诱导的HaCaT细胞凋亡的影响。方法：在DMEM 中培养HaCaT细胞，将细胞分成空白对照组(A组)，1mM咖啡因处理组(B组)，5mM咖啡因处理组(C组)，UVB(20mJ/cm2)辐射组(D组)，UVB辐射(20mJ/cm2)+1mM咖啡因处理组(E组)，UVB辐射(20mJ/cm2)+5mM咖啡因处理组(F组)，Annexin V/PI观察各组细胞凋亡比例，Western blot检测各组细胞p21和BCL-2表达情况。结果：20mJ/cm2UVB能诱导HaCaT细胞凋亡增加(A组 凋亡率5.43%，D组凋亡率20.67%)，而1mM和5mM咖啡因能进一步促进HaCaT细胞凋亡(E组凋亡率22.25%， F组凋亡率36.61%)，UVB 抑制细胞p21和BCL-2蛋白表达，咖啡因能进一步抑制21和BCL-2蛋白表达。结论：UVB辐射能诱导HaCaT细胞凋亡，抑制p21和BCL-2蛋白表达，咖啡因则能进一步促进凋亡，抑制p21和BCL-2蛋白表达。
[关键词]咖啡因；中波紫外线；细胞凋亡
[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455(2011)07-1105-03
The effect of caffeine to UVB irradiation induced cell apoptosis of HaCaT cells
LEI Xia1,LIU Bo2,HE Yu-ying3,WU Jin-jin1,CHENG Qiong-hui1
(1.Department of Dermatology,The Third Affiliated Hospital,The Third Military Medical University,Chongqing 400042,China；2.Department of Orthopaedics,The First Affiliated Hospital,Chongqing Medical University,Chonqing 400036,China；3.Department of Dermatology,University of Chicago,60637,USA)
Abstract:ObjectiveTo investigate the effect of caffeine on UVB irradiation induced cell apoptosis of HaCaT cells.MethodsHaCaT cells were cultured in DMEM medium. Cells were divided into 6 groups. A group: Blank control (without UVB and caffeine); B group:1mM caffeine, without UVB; C group: 5mM caffeine, without UVB; D group: without caffeine ,20mJ/cm2 UVB; E group: 1mM caffeine, 20mJ/cm2 UVB ; F group: 5mM caffeine, 20mJ/cm2 UVB. Annexin V/PIKit was used to detect cell apoptosis. Western blot was used to detect theexpression of p21 and BCL-2. Results 20mJ/cm2 UVB irradiation could induce cell apoptosis of HaCaT cells (cell apoptosis rate is 5.43% in group A and 20.67% in group D), 1mM and 5mM caffeine could promote cell apoptosis of HaCaT cells further (cell apoptosis rate is 22.25% in group E and 36.61% in group F). UVB irradiation could inhibite the expression of p21 and BCL-2,caffeine could inhibite p21 and BCL-2 futher.ConclusionsUVB irradiation could induce HaCaT cell apoptosis and inhibite the expression ofp21 and BCL-2 ......
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