TNF-α对体外培养的人牙囊细胞增殖特性及碱性磷酸酶的影响(1)
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[摘要]目的：检测不同浓度TNF- 对体外培养人牙囊细胞增殖活性和碱性磷酸酶活性的影响。方法：第5代人牙囊细胞接种于96孔培养板上，分别与不同浓度的TNF- 共同孵育，检测TNF-对人牙囊细胞的增殖活性和碱性磷酸酶活性的影响。结果：TNF-在浓度为10～50ng/ml，时间为3天时促进人牙囊细胞的增殖，浓度为10～200ng/ml时抑制碱性磷酸酶活性。结论：TNF-在特定的浓度和时间促进人牙囊细胞的增殖，抑制牙囊细胞的成骨特性。
[关键词]牙囊细胞；肿瘤坏死因子-α；增殖；碱性磷酸酶
[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455(2012)01-0071-03
Effects of TNF-α on the proliferation and alkaline phosphatase of the human dental follicle cells
BI Ying-chun1,Li Yun2,JIN Zuo-lin3
(1.Department of Stomatology,General Hospital of Jinan Military Area Command of Chinese PLA,Jinan 250031,Shandong,China;2.Department of Stomatology,210 Hospital of Shenyang Military Area Command of Chinese PLA；3.Department of Orthodontics,College of Stomatology,The Fourth Military Medical University,Xi'an 710032,Shaanxi,China)
Abstract: Objective To study the effects of different concentration TNF-α on the activity of proliferation and the activity of alkaline phosphatase of human dental follicle cells. Methods The 5th passage human dental follicle cells were seeded on 96-well plates and co-cultured with different concentration of TNF-α, the activity of proliferation and the activity of alkaline phosphatase was assayed. Results The 10～50ng/ml TNF-α enhanced the activity of proliferation profoundly and the effects were significant at 3 days after co-culture. 10～200ng/ml TNF-α decreased the activity of alkaline phosphatase. Conclusions TNF-α enhance the activity of proliferation and decreased the activity of alkaline phosphatase of human dental follicle cells at special concentration and time.
Key words:dental follicle cell; Tumor necrosis factor-α; proliferation; ALP activity
牙囊组织起源于外胚间充质，是包绕成釉器周围的疏松结缔组织，在牙齿萌出和牙周组织形成中起重要作用，缺乏牙囊的牙齿不能萌出，牙囊组织调节各种因子，如TNF-α、IL-10[1]、RANKLE等，吸引外周血中的单核细胞进入牙囊，融合成破骨细胞。TNF-α[2]第三天在大鼠牙囊中有微弱的表达，至第9天达到高峰，与第10天形成的破骨细胞高峰期有密切关系，TNF-α参与牙齿萌出的因子调节，是牙齿萌出过程中的重要因子。而TNF-α对人牙囊细胞的增殖的研究未见报道，这对TNF-α对人牙囊细胞生物学特性的影响有进一步的认识。
1 材料和方法
1.1 主要试剂与仪器：DMEM高糖培养基(Hyclone，USA)；胎牛血清(FBS，杭州四季青生物材料有限公司)；胰蛋白酶(GIBCO公司，进口分装)；TNF-α(Peprotech公司，USA)；96孔细胞培养板(NUCO ......
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