脂肪注射移植后皮肤质地改善的研究(1)
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[摘要]目的：通过皮下脂肪注射移植，探索移植脂肪对皮肤质地的改善作用。方法：取30只裸鼠，随机分成三组，所有鼠左侧皮下移植人脂肪细胞，在另一侧10只注射硅凝胶，10仅行皮下穿刺，剩下10只无任何处置，8周取皮肤组织做HE、Masson'染色观察皮肤真皮层厚度及真皮层内胶原蛋白的含量。结果：取材时，所有裸鼠上都能看到所移植的脂肪，但体积较术前有较明显缩小。肉眼看，脂肪呈正常外观，有较多新生血管生成。组织学检测，移植脂肪外周有大量细胞外基质形成，实验侧真皮层较对照侧明显增厚，真皮内胶原含量增多。结论：移植的脂肪组织不仅仅是一种填充物，在改善轮廓的同时，还有真皮增厚、胶原含量增加等皮肤改善的效应。
[关键词]脂肪注射移植；皮肤质地改善
[中图分类号]R622 [文献标识码]A [文章编号]1008-6455(2012)01-0078-04
Study on the Improvement of skin quality after fat tissue grafting: a animal studying
ZHU Shun-wu,SONG Guang-bing,XU Xue-wu,LIU Guo-feng
(Department of Plastic Surgery,2nd Hospital of Harbin Medical University Harbin,150086, Heilongjiang,China)
Abstract: Objective To investigate the histologic modifications of the skin after fat tissue grafting. Methods Thirty nude mice,divided into three groups randomly,were used in the experiment.All 30 mice received human fat tissue on left side.On the opposite side,10 mice received silicone gel,10 mice received only subcutaneous tunneling,and the remaining 10 mice received nothing (negative control group). Eight weeks later, biopsies of the skin and ubcutaneous tissue were performed and specimens were analyzed by hematoxylin-phloxin-saffron and Masson' staining. Dermis thickness was measured. Results Fat tissue was found in all animals. Macroscopically,fat tissue presented normal aspects,with abundant peripheral neovascularization.Histologic examination showed abundant extracellular matrix around the injected human fat tissue. Dermal thickness after fat grafting was significantly greater and collagen also increased significantly. Conclusions This study shows that fat tissue grafting stimulates a neosynthesis of collagen fibers at the recipient site and makes the dermis thicker. The effect it presented at the grafted area was not just volume-increasing but skin quality improvement.
Key words: fat tissue grafting; skin quality improvement
目前，自体脂肪组织被公认为最好的软组织填充材料之一，填充效果好，并发症出现率低。在临床应用中，有学者发现，自体脂肪组织注射移植不仅仅是一个填充物，在改善术区轮廓的同时，还有真皮增厚，真皮内胶原含量增加等皮肤质地改善的效应。然而，在人体做脂肪移植前后皮肤活检是难以接受的，因此我们以祼鼠为模型设计动物实验，在组织学上观察和比较脂肪移植前后皮肤质地的变化。
1 材料和方法
1.1材料
1.1.1 实验动物：选用 BALB/c裸鼠30只 ......
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