异体复合组织移植皮肤淋巴引流隔绝动物模型的建立(1)
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[摘要]目的：通过SD大鼠下腹部游离皮瓣及皮管的移植，建立大鼠异体复合组织移植皮肤淋巴引流隔绝模型。方法：7～8周龄雄性SD大鼠切取下腹部皮瓣并游离行同种异体移植，皮肤隔离组(A组)：将供体皮瓣边缘对合、缝合成管状，底边留血管蒂出口用隔离器隔离皮肤，显微外科吻合血管，皮管缝合于隔离器，隔离器固定于受体；皮肤未隔离组(B组)：将供体游离皮瓣卷制成管状，显微外科吻合血管并将供受体皮肤对位缝合；空白对照组(C组)：行下腹部游离皮瓣异体移植。大体观察每组皮瓣或皮管存活时间，于术后3、5、7、14、28、35d取移植物皮肤组织行HE染色,观察病理学变化。结果：A组：皮管存活时间为32(34，32)天；B组：皮管存活时间为15(16,15)天；C组：皮瓣存活时间为6(7,6)天。A组与C组、B组与C组、A组与B组存活率差异均有统计学意义(P<0.05)。结论：本实验成功建立了大鼠的异体复合组织移植淋巴引流隔绝模型,并证实了隔绝皮肤淋巴引流能有效延长移植物的存活时间。
[关键词]异体复合组织移植；免疫抑制；动物模型；淋巴引流
[中图分类号]R332 [文献标识码]A [文章编号]1008-6455(2012)04-0589-04
The establishing of composite tissue allograft skin lymphatic vessels isolation animal model in rats
LIU Sha,PEI Jiao-miao,LI Yang, CAO Jiao,YANG Yang，LI Bing，XIA Wei, GUO Shu-zhong
(Institute of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi,China)
Abstract: Objective To establish lymphatic vessels isolation animal model through vascularized hypogastric flap between SD rats. Methods 18 couples of SD rats(7-8weeks) were randomly divided into 3 groups. Group A(skin lymphatic vessles isolation): The free tube made of vascularized free flap located on the surface of the recipient through the isolation device after vascular anastomosis. Group B(tube) : The free tube made of vascularized free flap sutured on the recipient lower abdomen directly after vascular anastomosis. Group C(control) free flap allotransplantation. Observe the survive ratio of animal and rejection time of composite tissue flap, histologic outcomes on the day 3,5,7,14,28, and 35 after transplantation. Results The survival times of grafts in group A are 32(34, 32)days and group B were 15(16,15)days. By contrast,the survival time of group C was 6(7,6)days. The difference among these three groups were significant. Conclusions This experiment successfully established lymphatic vessels isolation animal model in SD rats. The prolonged survival times of the grafts in this animal model had been proved.
Key words: composite tissue allotransplantation；immunosuppression；animal model; lymph draining
器官移植已成功应用于临床50多年，然而异体复合组织移植却仍处于研究阶段，难以在临床上推广应用。皮肤的强抗原性和缺少有效安全的免疫抑制方案成为限制异体复合组织移植发展的主要因素[1-2] ......
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