SFRP2基因重组腺病毒载体的构建与鉴定(3)
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本研究构建SFRP2重组腺病毒载体,在293细胞中表达、包装、纯化病毒后成功构建了携带SFRP2重组腺病毒。并且初步研究结果表明该重组病毒在体外能有效感染正常增生性瘢痕成纤维细胞,受感染细胞能高效稳定的表达重组蛋白,为下一步研究SFRP2对病毒自身的复制与增殖以及宿主细胞中基因表达与调控、细胞凋亡等过程的机制奠定了基础。
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