一种稻米提取物的皮肤保湿和抗老化功能研究(1)
[摘要]目的：研究并验证一种稻米提取物(Aquarize IS)对丝聚蛋白(Filaggrin)、胶原蛋白I和胶原蛋白III生物合成的促进作用，及其对人体皮肤的保湿和抗老化功能。方法：对离体皮肤进行体外培养，每天分两次添加1% Aquarize IS 活性物作为活性物组，不添加活性物的设置为对照组，培养48h后依次进行组织切片和免疫荧光染色，拍照对比。临床人体测试选取健康志愿者21名，添加1%的Aquarize IS的基础霜作为活性物组，空白的基础霜作为安慰剂组进行对比。整个测试过程(6h)均在恒温恒湿实验室进行，选择前臂(手腕和肘部之间部分)屈侧作为测试部位，进行双盲的随机抽样试验。结果：离体皮肤试验证实Aquarize IS可以促进丝聚蛋白(Filaggrin)、胶原蛋白I和胶原蛋白III的合成。人体试验证实和安慰剂相比，含Aquarize IS的产品在1h，3h，6h增加皮肤水含量的比率分别为93%，85%和43%，且具有高显著性统计学差异(P≤0.01)。结论：稻米提取物(Aquarize IS)对丝聚蛋白(Filaggrin)，胶原蛋白I和胶原蛋白III生物合成具有促进作用，对人体皮肤有显著的保湿和抗老化功能。
[关键词]稻米提取物(Aquarize IS)；保湿；抗老化；丝聚蛋白；胶原蛋白
[中图分类号]R339.3+8 [文献标志码]A [文章编号]1008-6455(2015)04-0035-04
Efficacy of a rice extract (Aquarize IS) on skin moisturization and anti-aging
ZHAO Xiao-min，QU Xin
(ASI Shanghai Technical Center，Ashland Inc.，Shanghai 200233，China)
Abstract： Objective A new rice extract (Aquarize IS) will be evaluated for stimulating the synthesis of filaggrin，collagen I and collagen III， therefore providing skin moisturization and anti-aging effects. Methods 6mm punch biopsies of human skin (female) were cultured， apply samples with or without 1% aquarize IS twice per day. After 48h culturing， tissue slicing， immunofluorescence， picture taking were conducted to compare the efficacy. For in-vivo clinical test， 21 healthy Asian subjects were recruited， the inner sides of forearms were chosen as the test zone. The biofunctional side containing 1% Aquarize IS and the placebo without Aquarize IS were applied on the either side of forearm randomly. The whole test for 6h was performed in a controlled lab with constant temperature and humidity. Results 1% Aquarize IS can obviously improve the biosynthesis of filaggrin， collagen type I and collagen type III in skin. Comparing with the placebo， the product containing 1% Aquarize IS statistically significantly improved the skin surface hydration of 93%， 85% and 43% after 1 hour， 3 hours and 6 hours (P≤0.01). Conclusion 1% Aquarize IS showed good moisturization and anti-aging effect.
Key words：rice extract (Aquarize IS)； skin moisturization； anti-aging； filaggrin； collagen
皮肤保湿对角质层屏障功能的保护是至关重要的。过度洗涤、环境过于干燥或者环境污染影响等都会损伤角质层屏障功能，屏障功能的损伤会加速皮肤老化，增加皮肤的敏感度甚至引起一系列皮肤疾病。皮肤干燥的人，其脂质屏障从结构到组成都发生紊乱，而屏障的破坏增加了环境压力对皮肤的损伤。干性皮肤中，连接角质层的桥粒不能完成自身的降解程序，致使皮肤代谢功能异常而大量脱屑。同时角质层水合能力降低，通过皮肤的水分损失速度约比正常情况下大10倍，皮肤弹性减弱。一项法国的研究表明，无论其皮肤属于哪一类的女性，90%都有缺水的情况[1]。故而皮肤保湿也成为皮肤护理品和皮肤保健品的最基本功能，也是最主要和最被广泛需求的功能，因此越来越多的人从婴童时期就开始注重皮肤的保湿护理。本文通过应用离体皮肤测试(免疫组织化学法)和临床人体试验，来研究亚什兰公司的一种稻米提取物(Aquarize IS)对丝聚蛋白(Filaggrin)，胶原蛋白I和胶原蛋白III生物合成的促进作用，以及“由内而外”对人体皮肤产生保湿和抗老化的功能。 (赵小敏 瞿欣)
[关键词]稻米提取物(Aquarize IS)；保湿；抗老化；丝聚蛋白；胶原蛋白
[中图分类号]R339.3+8 [文献标志码]A [文章编号]1008-6455(2015)04-0035-04
Efficacy of a rice extract (Aquarize IS) on skin moisturization and anti-aging
ZHAO Xiao-min，QU Xin
(ASI Shanghai Technical Center，Ashland Inc.，Shanghai 200233，China)
Abstract： Objective A new rice extract (Aquarize IS) will be evaluated for stimulating the synthesis of filaggrin，collagen I and collagen III， therefore providing skin moisturization and anti-aging effects. Methods 6mm punch biopsies of human skin (female) were cultured， apply samples with or without 1% aquarize IS twice per day. After 48h culturing， tissue slicing， immunofluorescence， picture taking were conducted to compare the efficacy. For in-vivo clinical test， 21 healthy Asian subjects were recruited， the inner sides of forearms were chosen as the test zone. The biofunctional side containing 1% Aquarize IS and the placebo without Aquarize IS were applied on the either side of forearm randomly. The whole test for 6h was performed in a controlled lab with constant temperature and humidity. Results 1% Aquarize IS can obviously improve the biosynthesis of filaggrin， collagen type I and collagen type III in skin. Comparing with the placebo， the product containing 1% Aquarize IS statistically significantly improved the skin surface hydration of 93%， 85% and 43% after 1 hour， 3 hours and 6 hours (P≤0.01). Conclusion 1% Aquarize IS showed good moisturization and anti-aging effect.
Key words：rice extract (Aquarize IS)； skin moisturization； anti-aging； filaggrin； collagen
皮肤保湿对角质层屏障功能的保护是至关重要的。过度洗涤、环境过于干燥或者环境污染影响等都会损伤角质层屏障功能，屏障功能的损伤会加速皮肤老化，增加皮肤的敏感度甚至引起一系列皮肤疾病。皮肤干燥的人，其脂质屏障从结构到组成都发生紊乱，而屏障的破坏增加了环境压力对皮肤的损伤。干性皮肤中，连接角质层的桥粒不能完成自身的降解程序，致使皮肤代谢功能异常而大量脱屑。同时角质层水合能力降低，通过皮肤的水分损失速度约比正常情况下大10倍，皮肤弹性减弱。一项法国的研究表明，无论其皮肤属于哪一类的女性，90%都有缺水的情况[1]。故而皮肤保湿也成为皮肤护理品和皮肤保健品的最基本功能，也是最主要和最被广泛需求的功能，因此越来越多的人从婴童时期就开始注重皮肤的保湿护理。本文通过应用离体皮肤测试(免疫组织化学法)和临床人体试验，来研究亚什兰公司的一种稻米提取物(Aquarize IS)对丝聚蛋白(Filaggrin)，胶原蛋白I和胶原蛋白III生物合成的促进作用，以及“由内而外”对人体皮肤产生保湿和抗老化的功能。 (赵小敏 瞿欣)