机械牵张力加载时间对诱导正常皮肤成纤维细胞向增生性瘢痕成纤维细胞转化过程的影响研究(4)
综上所述，加载0.1Hz、10%拉伸幅度的机械牵张力作用5d时，NSFB表现出HSFB的典型特征，诱导转化的最佳加载时间为5d，可作为标准力学模型参数进一步研究增生性瘢痕形成机制及治疗方法。此外，在加载机械牵张力7d时，NSFB的各分子表达水平已超出未加载机械牵张力的HSFB，虽然不可否认此时的NSFB未停止向HSFB转化，但已超出正常HSFB的标准表征，可能会发生过度转化，仍需进一步实验研究过度加载机械牵张力会对成纤维细胞产生何种功能上的影响甚至表型的影响。
[参考文献]
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, http://www.100md.com
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[11]Zhao Z，Tan SH，Hiroaki M，et al.Association between tensin 1 and p130Cas at focal adhesions links actin inward flux to cell migration[J].Biol Open，2016，5(4)：499-506.
, 百拇医药
[12]Sawada Y，Hirata H.p130Cas-Mediated Regulation of Mechanical Functions of Cells[J].Clin Calcium，2016，26(12)：1743-1749.
[13]Sun Z，Guo SS，F?ssler R.Integrin-mediated mechanotransduction[J].J Cell Biol，2016，215(4)：445-456.
[14]Tajik A，Zhang Y，Wei F，et al.Transcription upregulation via force-induced direct stretching of chromatin[J].Nat Mater，2016，15(12)：1287-1296.
[15]Fan C，Dong Y，Xie Y，et al.Shikonin reduces TGF-β1-induced collagen production and contraction in hypertrophic scar-derived human skin fibroblasts[J].Int J Mol Med，2015，36(4)：985-991.
, 百拇医药
[16]Huang C，Ogawa R.The link between hypertension and pathological scarring： does hypertension cause or promote keloid and hypertrophic scar pathogenesis？[J].Wound Repair Regen，2014，22(4)：462-466.
[17]Liu S，Jiang L，Li H，et al.Mesenchymal stem cells prevent hypertrophic scar formation via inflammatory regulation when undergoing apoptosis[J].J Invest Dermatol，2014，134(10)：2648-2657.
[收稿日期]2018-01-29 [修回日期]2018-05-15
編辑/朱婉蓉, 百拇医药(朱明宇 李雨珊 王志国)
[参考文献]
[1]Hu M，Che P，Han X，et al.Therapeutic targeting of SRC kinase in myofibroblast differentiation and pulmonary fibrosis[J].J Pharmacol Exp Ther，2014，351(1)：87-95.
, http://www.100md.com [2]Barnes LA，Marshall CD，Leavitt T，et al.Mechanical Forces in Cutaneous Wound Healing： Emerging Therapies to Minimize Scar Formation[J].Adv Wound Care，2017，7(2)：47-56..
[3]王志国，匡瑞霞，陈振雨，等.不同幅度牵张力对正常皮肤成纤维细胞向增生性瘢痕成纤维细胞转化的诱导作用[J].中华医学杂志，2015，95(4)：294-298.
[4]李鹏，王志国.机械张力参与病理性瘢痕形成的研究进展[J].中国美容整形外科杂志，2013，24(11)：655-657.
[5]Zhi-Guo W，Quan-Chen X，Rui-Xia K，et al.Principles of hatchet-skin flap for repair of tissue defects on the cheek[J].Aesthetic Plast Surg，2012，36(1)：163-168.
, http://www.100md.com
[6]Anwar MA，Shalhoub J，Lim CS，et al.The effect of pressure-induced mechanical stretch on vascular wall differential gene expression[J].J Vasc Res，2012，49(6)：463-478.
[7]Zaman，Kamarul T.An investigation of tendon fibroblast responses to tensile loading in vitro[D].University of Liverpool，2011.
[8] Struewer J，Roessler PP，Schuettler KF，et al.Influence of cyclical mechanical loading on osteogenic markers in an osteoblast-fibroblast co-culture in vitro： tendon-to-bone interface in anterior cruciate ligament reconstruction[J].Int Orthop，2014，38(5)：1083-1089.
, http://www.100md.com
[9]Roa-Espitia AL，Hernández-Rendón ER，Baltiérrez-Hoyos R，et al.Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation[J].Biol Open，2016，5(9)：1189-1199.
[10]Hasnat MA，Pervin M，Lim JH，et al.Apigenin Attenuates Melanoma Cell Migration by Inducing Anoikis through Integrin and Focal Adhesion Kinase Inhibition[J].Molecules，2015，20(12)：21157-21166.
[11]Zhao Z，Tan SH，Hiroaki M，et al.Association between tensin 1 and p130Cas at focal adhesions links actin inward flux to cell migration[J].Biol Open，2016，5(4)：499-506.
, 百拇医药
[12]Sawada Y，Hirata H.p130Cas-Mediated Regulation of Mechanical Functions of Cells[J].Clin Calcium，2016，26(12)：1743-1749.
[13]Sun Z，Guo SS，F?ssler R.Integrin-mediated mechanotransduction[J].J Cell Biol，2016，215(4)：445-456.
[14]Tajik A，Zhang Y，Wei F，et al.Transcription upregulation via force-induced direct stretching of chromatin[J].Nat Mater，2016，15(12)：1287-1296.
[15]Fan C，Dong Y，Xie Y，et al.Shikonin reduces TGF-β1-induced collagen production and contraction in hypertrophic scar-derived human skin fibroblasts[J].Int J Mol Med，2015，36(4)：985-991.
, 百拇医药
[16]Huang C，Ogawa R.The link between hypertension and pathological scarring： does hypertension cause or promote keloid and hypertrophic scar pathogenesis？[J].Wound Repair Regen，2014，22(4)：462-466.
[17]Liu S，Jiang L，Li H，et al.Mesenchymal stem cells prevent hypertrophic scar formation via inflammatory regulation when undergoing apoptosis[J].J Invest Dermatol，2014，134(10)：2648-2657.
[收稿日期]2018-01-29 [修回日期]2018-05-15
編辑/朱婉蓉, 百拇医药(朱明宇 李雨珊 王志国)
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