白细胞介素24和替尼泊甙联合抑制人胶质瘤细胞增殖的机制研究(1)
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(1汕头大学附属粤北人民医院妇科 韶关 512026；
2河北省人民医院功能神经外科；3河北省人民医院临床研究中心 石家庄 050071)
【摘要】目的：观察白细胞介素24(IL-24)与化疗药替尼泊甙(VM-26)对人胶质瘤细胞增殖的抑制作用是否相互协同增效，并探讨其可能的作用机制。方法：先用载有人白细胞介素24cDNA的新型重组腺病毒(Ad5F35-IL24)感染人胶质瘤细胞系U251，接着加入替尼泊甙。观察IL-24蛋白表达水平、细胞抑制率、凋亡率及Bcl-2和Bax蛋白的表达情况。结果：Ad5F35-IL24感染U251细胞后IL-24蛋白高表达，Ad5F35-IL24+VM-26组U251细胞的抑制率、凋亡率明显增高(P<0.001)，Ad5F35-IL24+VM-26组U251细胞的Bax/Bcl-2升高最明显(P<0.05)。结论：IL-24和替尼泊甙对U251细胞具有协同杀伤和诱导凋亡的作用，其机制可能与促凋亡蛋白Bax和抗凋亡蛋白Bcl-2的比值的升高有关。
【关键词】白细胞介素24；胶质瘤细胞；腺病毒载体；化疗增敏；信号转导
The Inhibitory Effect of IL-24 and VM-26 on Cell Gowth of Human m alignant glioma
Wang Yuehong1 Zhao Wenqing2 Li Wenling2 Xu HongYan1 Liang Chuandong2 Zhao Xiaoyun3
【Abstract】 Objective: We want to observe that Combination of interleukin-24 and chemotherapy medicine VM-26 is synergy or no synergy and to approach its potential mechanism of the action. Methods: After administration of Ad5F35-IL24 and VM-26 to U251 cell, we detected their expression of IL-24 protein with western blot and inhibition rate and expression of Bax/Bcl-2 protein. Results: Expression of IL-24 proteins were expressed highly after inflection of Ad5F35-IL24. while U251 cells were administrated with Ad5F35-IL24 and VM-26, their inhibition rate and apoptosis and expression of Bax/Bcl-2 protein were significantly raised up(P<0.05). Conclusions: IL-24 and VM-26 synergisticly inhibit U251 cells’ proliferation and induce their apoptosis, which may be related to the increased ratio of the pro-apoptotic protein Bax to anti-apoptosis protein Bcl-2.
【Key words】 Interleukin 24; Glial cells; Viral vector; Chemotherapy radiosensitizing; Signal transduction
【中图分类号】R739.41 【文献标识码】A 【文章编号】1008-6455(2011)06-0346-02
白细胞介素24(IL-24)因其独特的选择性抗肿瘤作用而成为研究热点 ......
(1汕头大学附属粤北人民医院妇科 韶关 512026；
2河北省人民医院功能神经外科；3河北省人民医院临床研究中心 石家庄 050071)
【摘要】目的：观察白细胞介素24(IL-24)与化疗药替尼泊甙(VM-26)对人胶质瘤细胞增殖的抑制作用是否相互协同增效，并探讨其可能的作用机制。方法：先用载有人白细胞介素24cDNA的新型重组腺病毒(Ad5F35-IL24)感染人胶质瘤细胞系U251，接着加入替尼泊甙。观察IL-24蛋白表达水平、细胞抑制率、凋亡率及Bcl-2和Bax蛋白的表达情况。结果：Ad5F35-IL24感染U251细胞后IL-24蛋白高表达，Ad5F35-IL24+VM-26组U251细胞的抑制率、凋亡率明显增高(P<0.001)，Ad5F35-IL24+VM-26组U251细胞的Bax/Bcl-2升高最明显(P<0.05)。结论：IL-24和替尼泊甙对U251细胞具有协同杀伤和诱导凋亡的作用，其机制可能与促凋亡蛋白Bax和抗凋亡蛋白Bcl-2的比值的升高有关。
【关键词】白细胞介素24；胶质瘤细胞；腺病毒载体；化疗增敏；信号转导
The Inhibitory Effect of IL-24 and VM-26 on Cell Gowth of Human m alignant glioma
Wang Yuehong1 Zhao Wenqing2 Li Wenling2 Xu HongYan1 Liang Chuandong2 Zhao Xiaoyun3
【Abstract】 Objective: We want to observe that Combination of interleukin-24 and chemotherapy medicine VM-26 is synergy or no synergy and to approach its potential mechanism of the action. Methods: After administration of Ad5F35-IL24 and VM-26 to U251 cell, we detected their expression of IL-24 protein with western blot and inhibition rate and expression of Bax/Bcl-2 protein. Results: Expression of IL-24 proteins were expressed highly after inflection of Ad5F35-IL24. while U251 cells were administrated with Ad5F35-IL24 and VM-26, their inhibition rate and apoptosis and expression of Bax/Bcl-2 protein were significantly raised up(P<0.05). Conclusions: IL-24 and VM-26 synergisticly inhibit U251 cells’ proliferation and induce their apoptosis, which may be related to the increased ratio of the pro-apoptotic protein Bax to anti-apoptosis protein Bcl-2.
【Key words】 Interleukin 24; Glial cells; Viral vector; Chemotherapy radiosensitizing; Signal transduction
【中图分类号】R739.41 【文献标识码】A 【文章编号】1008-6455(2011)06-0346-02
白细胞介素24(IL-24)因其独特的选择性抗肿瘤作用而成为研究热点 ......
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