定痫丸对戊四唑点燃癫痫大鼠脑内神经递质含量及海马c-fos表达的影响(1)
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(江苏省中医院，江苏 南京 210029)
摘 要：目的：通过观察定痫丸对癫痫大鼠神经递质谷氨酸(Glu)、γ-氨基丁酸(GABA)和海马c-fos表达的影响来探讨该方治疗癫痫的机理。方法：采用腹腔注射戊四唑3.2mg/100g，连续给予4周造成大鼠癫痫模型，造模同时给予定痫丸、丙戊酸钠和定痫丸加丙戊酸钠治疗，连续4周。结果：采用戊四唑造模后，模型组大鼠脑组织中谷氨酸含量升高而γ-氨基丁酸含量明显降低，c-fos阳性细胞数明显增多，与正常组比较有显著性差异(P<0.05)。定痫丸组、丙戊酸钠组和中西药合用组均可明显降低谷氨酸含量，升高γ-氨基丁酸含量，减少c-fos阳性细胞数，与模型组比较具有显著性差异(P<0.05)。结论：定痫丸可对抗戊四唑致大鼠癫痫的作用,作用机理与降低脑内神经递质谷氨酸含量、升高γ-氨基丁酸含量以及阻断脑内c-fos蛋白的表达有关。
关键词：定痫丸；戊四唑；谷氨酸和γ-氨基丁酸；海马c-fos表达
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)03-0468-03
Effect of Dingxianwan on the Concent of Gaba Glu in Brains and the
Expression of C-FOS in Hippocampus in
Ippocampus in PTZ-kindled Epilepsy Rats
ZHU Xuan-xuan,DAI Bing,YIN Kun,WANG Wei-zhong
(Jiangsu Hospital of TCM,Nanjing 210029,Jiangsu,China)
Abstract:Objective：Observe effect of Dingxianwan on quantity of GABA,Glu and c-fos in hippocampus so that we probe into the efficacy. Methods：Intraperitoneal injection of pentylenetetrazol 3.2mg/100g, consecutive to cause epilepsy in 4 weeks, At the same time to be cure by Dingxianwan,VPA, Dingxianwan combined with VPA. Results：After modeling by PIZ,model group were increased in brain tissue Glu, GABA concentration decreased, c-fos positive cells increased significantly. Dingxianwan group, VPA group and the Chinese and western medicine group were decreased Glu content, increased GABA levels, reduce the number of c-fos positive cells, which was indistinctive compared with model group. Conclusion：Dingxianwan can resist obviously seizure from PTZ-kindlod rats,its function mechanism may be that react by reducing the content of Glu and expression of c-fos in the hippocampi and increasing the content of GABA of PTZ-kindled rats.
Key words:Dingxianwan;PIZ-kindled Modle;Glu;GABA;C-fos In Hippocampus
癫痫是由多种病因引起的慢性脑部疾病 ......
(江苏省中医院，江苏 南京 210029)
摘 要：目的：通过观察定痫丸对癫痫大鼠神经递质谷氨酸(Glu)、γ-氨基丁酸(GABA)和海马c-fos表达的影响来探讨该方治疗癫痫的机理。方法：采用腹腔注射戊四唑3.2mg/100g，连续给予4周造成大鼠癫痫模型，造模同时给予定痫丸、丙戊酸钠和定痫丸加丙戊酸钠治疗，连续4周。结果：采用戊四唑造模后，模型组大鼠脑组织中谷氨酸含量升高而γ-氨基丁酸含量明显降低，c-fos阳性细胞数明显增多，与正常组比较有显著性差异(P<0.05)。定痫丸组、丙戊酸钠组和中西药合用组均可明显降低谷氨酸含量，升高γ-氨基丁酸含量，减少c-fos阳性细胞数，与模型组比较具有显著性差异(P<0.05)。结论：定痫丸可对抗戊四唑致大鼠癫痫的作用,作用机理与降低脑内神经递质谷氨酸含量、升高γ-氨基丁酸含量以及阻断脑内c-fos蛋白的表达有关。
关键词：定痫丸；戊四唑；谷氨酸和γ-氨基丁酸；海马c-fos表达
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)03-0468-03
Effect of Dingxianwan on the Concent of Gaba Glu in Brains and the
Expression of C-FOS in Hippocampus in
Ippocampus in PTZ-kindled Epilepsy Rats
ZHU Xuan-xuan,DAI Bing,YIN Kun,WANG Wei-zhong
(Jiangsu Hospital of TCM,Nanjing 210029,Jiangsu,China)
Abstract:Objective：Observe effect of Dingxianwan on quantity of GABA,Glu and c-fos in hippocampus so that we probe into the efficacy. Methods：Intraperitoneal injection of pentylenetetrazol 3.2mg/100g, consecutive to cause epilepsy in 4 weeks, At the same time to be cure by Dingxianwan,VPA, Dingxianwan combined with VPA. Results：After modeling by PIZ,model group were increased in brain tissue Glu, GABA concentration decreased, c-fos positive cells increased significantly. Dingxianwan group, VPA group and the Chinese and western medicine group were decreased Glu content, increased GABA levels, reduce the number of c-fos positive cells, which was indistinctive compared with model group. Conclusion：Dingxianwan can resist obviously seizure from PTZ-kindlod rats,its function mechanism may be that react by reducing the content of Glu and expression of c-fos in the hippocampi and increasing the content of GABA of PTZ-kindled rats.
Key words:Dingxianwan;PIZ-kindled Modle;Glu;GABA;C-fos In Hippocampus
癫痫是由多种病因引起的慢性脑部疾病 ......
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