麻杏石甘汤对A型流感病毒感染小鼠IL-2\IL-4蛋白表达水平的影响(1)
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(湖南中医药大学，湖南 长沙 410208)
摘 要：目的：观察麻杏石甘汤对A型流感病毒感染小鼠IL-2、IL-4蛋白表达水平的影响。方法：采用鼻腔接种法建立A型流感病毒小鼠肺适应株动物感染模型。实验设正常对照组、模型对照组、抗病毒颗粒组、利巴韦林组和麻杏石甘汤(MSXGD)临床等效剂量组、2倍临床等效剂量组。经灌胃给药或给生理盐水7天后，用ELISA法检测小鼠血清中IL-2、IL-4的含量水平，用免疫组织化学法定位与半定量检测小鼠肺组织中IL-2、IL-4的蛋白表达水平。结果：流感病毒感染小鼠后，IL-2、IL-4的血清含量水平和肺组织蛋白表达水平降低，与正常对照组比较，差异显著(P<0.01)。MXSGD临床等效剂量组、2倍临床等效剂量组小鼠IL-2、IL-4的血清含量水平和肺组织蛋白表达水平升高，与模型对照组比较，差异显著(P<0.01或P<0.05)。结论：麻杏石甘汤通过调节一些IL-2、IL-4的蛋白表达水平,对机体抗感染免疫发挥一定调节作用。
关键词：麻杏石甘汤；A型流感病毒；IL-2；IL-4
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)03-0475-03
The Effects of Maxing Shigan Decoction on the Protein Levels of IL-2 and
IL-4 of Mice Infected by A-type Influenza Virus
LU Fang-guo，ZHANG Bo，YAN Jie，LI Ling，ZHU Ying-wu，LI Shan，XIANG Qin，PAN Kun
(Hunan University of TCM, Changsha 410208,Hunan,China)
Abstract:Objective：To observe effects of Maxing Shigan Decoction(MXSGD) on the protein levels of IL-2 and IL-4 of mice infected by A-type influenza virus in their serum and lung tissue. Methods: The mice model of pulmonary is established by nasal inoculating A-type influenza virus, Set up normal control group, model control group, Anti-virus particle group, ribavirin group, MXSGD clinical equivalent dose group, two times clinical equivalent dose group. After gavaging drug or normal saline, To detect the levels of IL-2 and IL-4 in serum and lung tissue. Result: The levels of IL-2 and IL-4 in serum and lung tissue for mice infected by A-type influenza virus reduced, compared with the normal control group, significant difference (P<0.01). The levels of IL-2 and IL-4 increased in MXSGD clinical equivalent dose group and two times clinical equivalent dose group, compared with the model control group, significant difference (P<0.01, P<0.05). Conclusion: By affecting the expression of IL-2 and IL-4 in the body, MXSGD play a certain anti-infective immune regulatory role.
Key words:Maxing Shigan Decoction; A-type influenza virus; IL-2; IL-4
流感病毒(influenza Virus)有 A(甲)、B(乙)、C(丙)三型 ......
(湖南中医药大学，湖南 长沙 410208)
摘 要：目的：观察麻杏石甘汤对A型流感病毒感染小鼠IL-2、IL-4蛋白表达水平的影响。方法：采用鼻腔接种法建立A型流感病毒小鼠肺适应株动物感染模型。实验设正常对照组、模型对照组、抗病毒颗粒组、利巴韦林组和麻杏石甘汤(MSXGD)临床等效剂量组、2倍临床等效剂量组。经灌胃给药或给生理盐水7天后，用ELISA法检测小鼠血清中IL-2、IL-4的含量水平，用免疫组织化学法定位与半定量检测小鼠肺组织中IL-2、IL-4的蛋白表达水平。结果：流感病毒感染小鼠后，IL-2、IL-4的血清含量水平和肺组织蛋白表达水平降低，与正常对照组比较，差异显著(P<0.01)。MXSGD临床等效剂量组、2倍临床等效剂量组小鼠IL-2、IL-4的血清含量水平和肺组织蛋白表达水平升高，与模型对照组比较，差异显著(P<0.01或P<0.05)。结论：麻杏石甘汤通过调节一些IL-2、IL-4的蛋白表达水平,对机体抗感染免疫发挥一定调节作用。
关键词：麻杏石甘汤；A型流感病毒；IL-2；IL-4
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)03-0475-03
The Effects of Maxing Shigan Decoction on the Protein Levels of IL-2 and
IL-4 of Mice Infected by A-type Influenza Virus
LU Fang-guo，ZHANG Bo，YAN Jie，LI Ling，ZHU Ying-wu，LI Shan，XIANG Qin，PAN Kun
(Hunan University of TCM, Changsha 410208,Hunan,China)
Abstract:Objective：To observe effects of Maxing Shigan Decoction(MXSGD) on the protein levels of IL-2 and IL-4 of mice infected by A-type influenza virus in their serum and lung tissue. Methods: The mice model of pulmonary is established by nasal inoculating A-type influenza virus, Set up normal control group, model control group, Anti-virus particle group, ribavirin group, MXSGD clinical equivalent dose group, two times clinical equivalent dose group. After gavaging drug or normal saline, To detect the levels of IL-2 and IL-4 in serum and lung tissue. Result: The levels of IL-2 and IL-4 in serum and lung tissue for mice infected by A-type influenza virus reduced, compared with the normal control group, significant difference (P<0.01). The levels of IL-2 and IL-4 increased in MXSGD clinical equivalent dose group and two times clinical equivalent dose group, compared with the model control group, significant difference (P<0.01, P<0.05). Conclusion: By affecting the expression of IL-2 and IL-4 in the body, MXSGD play a certain anti-infective immune regulatory role.
Key words:Maxing Shigan Decoction; A-type influenza virus; IL-2; IL-4
流感病毒(influenza Virus)有 A(甲)、B(乙)、C(丙)三型 ......
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