化瘀通络方对局灶性脑缺血大鼠再灌注(MCAO)后脑组织氧自由基和NOS表达的影响(1)
参见附件(12kb)。
(1.南京中医药大学附属医院，江苏 南京 210029；2.南京中医药大学，江苏 南京 210029)
摘 要：目的：观察化瘀通络方治疗大鼠局灶性脑缺血-再灌注损伤对脑组织自由基及一氧化氮合成酶(NOS)和iNOS变化的影响。方法：制备大鼠局灶性脑缺血再灌注损伤模型，给予化瘀通络方治疗后观察丙二醛(MDA)、超氧化物歧化酶(SOD)及一氧化氮合成酶(NOS)和诱导型一氧化氮合成酶(iNOS)的变化。结果：化瘀通络方可显著降低MDA含量以及iNOS和NOS活性，SOD含量升高。结论：在脑缺血再灌注损伤后, 化瘀通络方能抑制脑缺血再灌注后脂质过氧化反应，促进自由基清除，对抗自由基损伤，提高脑组织自身抗氧化能力，降低NOS和iNOS介导脑缺血-再灌注时的神经损伤，保护脑细胞。
关键词：化瘀通络方；脑缺血-再灌注损伤模型；SOD；MDA；NOS；iNOS
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)03-0509-03
Effect of Huayu Tongluo Fang on the Expression of ROS and NOS in Brain
Tissue During the Reperfusion After Middle Cerebral Artery Occlusion in Rats
QIU Zhao-juan1，ZHU Xuan-xuan1，DA Qing-guo1，WU Xu-tong1-ZHU Wu-yuan2
(1.Affiliated Hospital of Nanjing University of Chinese Medicine，Nanjiang 210029,Jiangsu,China;
2.Nanjing University of Chinese Medicine, Nanjing 210029,Jiangsu,China)
Abstract:Objective:To observe the effect of huayutongluofang on expression of ROS,NOS and iNOS during the reperfusion after middle cerebral artery occlusion in rats. Methods:The cerebral IR rats model were established by middle cerebral artery occlusion.Treated the cerebral IR rats with huayutongluofang,then observing the changes of MDA,SOD,NOS and iNOS in brain tissue. Results:Huayutongluofang could reduce the expression of MDA,iNOS and NOS in brain tissue.While, after huayutongluofang treatment,the SOD level in brain tissue was increased.Conclusions:During the reperfusion after middle cerebral artery occlusion,huayutongluofang can restrain the Lipid Peroxidation,promote removal of oxygen free radicals, compete oxygen-free radical injury. While,runhoukaiyinkeli could reduce the possibility of nerve damage,protect neural cells from injured.
Key words:Huayutongluofang;cerebral IR rats model;SOD;MDA;NOS;iNOS
收稿日期：2010-09-06
基金项目：江苏省中药药效与安全性评价重点实验室资助项目(P09017)；江苏省中医药管理局资助项目(LZ09048)
作者简介：邱召娟(1966-) ......
(1.南京中医药大学附属医院，江苏 南京 210029；2.南京中医药大学，江苏 南京 210029)
摘 要：目的：观察化瘀通络方治疗大鼠局灶性脑缺血-再灌注损伤对脑组织自由基及一氧化氮合成酶(NOS)和iNOS变化的影响。方法：制备大鼠局灶性脑缺血再灌注损伤模型，给予化瘀通络方治疗后观察丙二醛(MDA)、超氧化物歧化酶(SOD)及一氧化氮合成酶(NOS)和诱导型一氧化氮合成酶(iNOS)的变化。结果：化瘀通络方可显著降低MDA含量以及iNOS和NOS活性，SOD含量升高。结论：在脑缺血再灌注损伤后, 化瘀通络方能抑制脑缺血再灌注后脂质过氧化反应，促进自由基清除，对抗自由基损伤，提高脑组织自身抗氧化能力，降低NOS和iNOS介导脑缺血-再灌注时的神经损伤，保护脑细胞。
关键词：化瘀通络方；脑缺血-再灌注损伤模型；SOD；MDA；NOS；iNOS
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)03-0509-03
Effect of Huayu Tongluo Fang on the Expression of ROS and NOS in Brain
Tissue During the Reperfusion After Middle Cerebral Artery Occlusion in Rats
QIU Zhao-juan1，ZHU Xuan-xuan1，DA Qing-guo1，WU Xu-tong1-ZHU Wu-yuan2
(1.Affiliated Hospital of Nanjing University of Chinese Medicine，Nanjiang 210029,Jiangsu,China;
2.Nanjing University of Chinese Medicine, Nanjing 210029,Jiangsu,China)
Abstract:Objective:To observe the effect of huayutongluofang on expression of ROS,NOS and iNOS during the reperfusion after middle cerebral artery occlusion in rats. Methods:The cerebral IR rats model were established by middle cerebral artery occlusion.Treated the cerebral IR rats with huayutongluofang,then observing the changes of MDA,SOD,NOS and iNOS in brain tissue. Results:Huayutongluofang could reduce the expression of MDA,iNOS and NOS in brain tissue.While, after huayutongluofang treatment,the SOD level in brain tissue was increased.Conclusions:During the reperfusion after middle cerebral artery occlusion,huayutongluofang can restrain the Lipid Peroxidation,promote removal of oxygen free radicals, compete oxygen-free radical injury. While,runhoukaiyinkeli could reduce the possibility of nerve damage,protect neural cells from injured.
Key words:Huayutongluofang;cerebral IR rats model;SOD;MDA;NOS;iNOS
收稿日期：2010-09-06
基金项目：江苏省中药药效与安全性评价重点实验室资助项目(P09017)；江苏省中医药管理局资助项目(LZ09048)
作者简介：邱召娟(1966-) ......
您现在查看是摘要介绍页，详见PDF附件(12kb)。